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TAVUKLARDA BİSFENOL A VE DİETİLSTİLBESTROL’ÜN BURSA FABRİCİUS’UN İNVOLUSYONU ÜZERİNE ETKİSİ

Effects of Bisphenol A and Diethylstilbestrol on the Involution of Bursa of Fabricius in the Hens

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2013

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The bursa of Fabricius is a primary lymphoid organ in birds responsible for B lymphocyte maturation. Sex steroid hormones influence regression of the bursa of Fabricius. Bisphenol A (BPA) is an estrogenic chemical that is widely used in the manufacture of plastics and epoxy resins. This experiment was conducted to determine the effects of BPA on the involution of bursa of Fabricius in the adult hens. The synthetic estrogen diethylstilbestrol (DES) was used as a positive control. The test compounds were injected into the yolk of embryonated eggs. Immunohistochemical procedure performed to determine estrogen receptor alpha (ERα) expression in bursa of Fabricius. In high dose BPA (134 μg/g egg) and DES (0.2 μg/g egg) groups, the bursa somatic index, the number and diameter of the follicles in bursa of Fabricius were statistically decreased compared to control and low dose BPA (67 μg/g egg) and DES (0.02 μg/g egg) groups (P<0.05). There were no specific immune reactions for ERα in bursa of Fabricius in all groups. These results suggest that embryonic exposure to BPA and DES induced the regression of the bursa of Fabricius in hens.
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Abstract (Original Language): 
Bursa Fabricius kuşlarda B lenfositlerin olgunlaşmasından sorumlu primer (birincil) lenfoid organdır. Seks steroid hormonları bursa Fabricius’un gerilemesini etkiler. Bisfenol A (BPA), plastik ve epoksi reçine üretiminde yaygın olarak kullanılan bir östrojenik bir kimyasaldır. Bu çalışma, BPA’nın ergin tavuklarda bursa Fabricius’un involusyonu üzerine etkisini belirlemek amacıyla yapıldı. Pozitif kontrol olarak sentetik östrojen olan dietilstilbestrol (DES) kullanıldı. Test bileşikleri embriyolu yumurta sarısının içine enjekte edildi. Bursa Fabricius’da östrojen alfa reseptörü (ÖRα) ekspresyonunu belirlemek için immunohistokimyasal yöntem kullanıldı. Yüksek doz BPA (134 mg/g yumurta) ve DES (0,2 mg/g yumurta) gruplarında, bursa somatik indeks, bursa Fabricius’un follikül sayısı ve çapı, kontrol ve düşük doz BPA (67 μg/g yumurta) ve DES (0,02 μg/g/ yumurta) gruplarına göre istatistiksel olarak azalmıştı (P<0,05). Tüm gruplarda bursa Fabricius’da spesifik ÖRα immun reaksiyon yoktu. Bu sonuçlar, embriyonik dönemde BPA ve DES’e maruz kalan tavuklarda bursa Fabricius’un gerilemesini indüklediğini göstermektedir.
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  • 2
168-174
  • English

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