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ANNEDEN AYRILMA VE TEK TEK BARINDIRILMA STRESİNİN SIÇANLARIN FİZİKSEL VE MENTAL GELİŞİMLERİ ÜZERİNE ETKİLERİ

THE AFFECT OF SEPERATION AND BEING AWAY FROM THE MOTHER ON RATS PUPPIES’ PHYSICAL AND MENTAL DEVELOPMENT

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DOI: 
http://dx.doi.org/10.9761/JASSS_645

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Abstract (2. Language): 
The aim of this study is to find out the effects on physical and mental development at rats that are separated from mothers and put into single housing. This study was conducted in Experimental Animal Research Center of Medical Faculty of Dokuz Eylül University. The ethical approval was taken from Dokuz Eylül University Animal Experiments Ethical Committee. In the study totally 28 rat puppies, 12 male and 16 female born from three Wistar Albino rats were used. 1st group (n: 6) the male rats puppies that have being away from the mother stress. 2nd group (n: 8) the female rats puppies that have being away from the mother stress. 3rd group (n: 6) the male rats puppies who don’t have separation stress. 4th group (n: 8) the female rats puppies who don’t have separation stress. All the puppy rats lived with their mother till the 18th day of their birth. The rats in 1st and 2 nd groups were taken from their mother between 18-30 th days, totally 12 days, 4 hours every day at the same time and same situation. Sucking from the mother was cut at the 30 th day and the puppy rats that are separated from the mothers at the 1 st and 2 nd groups were put to single housing between 30-90 days. On the other hand the other groups who don’t have separation stress were put in cages as males to get his and females to get her. The weight gain, food and water consumption was measured once a week to check out the physical development and water tank test was applied at 30 th and 90 th day to follow the mental development. There was a statistically significant difference (P< 0,05) between the groups that had separation stress and that don’t have this stress when the weight gain and food and water consumption and learning and remembering faculty were compared. At the beginning of the experiment the rats that are separated from the mothers and put in to single housing, who have stress at both races had fright, anxiety, uneasiness, decrease in food and water consumption and all this affected their physical development. But as the time passed, in later weeks the rats that were in stress adapted to the new situation and these rats became more researchers and interfering then the rat groups who don’t have stress. The physical and mental development of the separation stress group was not affected totally negatively as we thought at the beginning of the hypothesis, adversely more positive permanent changes have occurred. When the results of this study is adapted to the effect on the population, by diminishing the protective effect of the parents on children’s development and raising may have positive effect on children’s mental and physical development, but this does not mean that they should be left to the streets.
Abstract (Original Language): 
Bu çalışmada amaç anneden ayrılma stresi ve ardından tek tek ayrı barındırmanın sıçanlarda fiziksel ve mental gelişim üzerine etkilerini araştırmaktır. Bu amaçla anneden ayrılma stresi ve tek tek barındırma stresi yaşayan sıçanlar ile bu stresi yaşamayan sıçanlar olmak üzere iki grup karşılaştırılmıştır. Çalışmada 3 adet hamile Wistar Albino sıçandan doğan 28 adet yavru sıçan kullanılmıştır. Bütün yavrular doğumdan sonra 18. güne kadar anneleri ile birlikte yaşamışlardır. 18.-30. günler arası her annenin yavrularının, yavru sayısı ve cinsiyeti göz önüne alınarak ve randomize olarak seçilmiş yavruların yarısı anneleri ile birlikte yaşamaya devam ederken, diğer yarısı annelerinden ayrılarak günde 4 saat uygun ortamda annelerinden ayrılma stresine maruz bırakılmıştır. 28 sıçan: I. grup (n:6) anneden ayrılma stresi yaşayan ve tek tek ayrı kafeslerde barındırılan erkek sıçanlar, 2. grup (n:8) anneden ayrılma stresi yaşayan ve tek tek ayrı kafeslerde barındırılan dişi sıçanlar, 3. grup (n:6) anne ile birlikte yaşayan ve toplu olarak tek bir kafeste barındırılan erkek sıçanlar ve 4. grup (n:8) anne ile birlikte yaşayan ve toplu olarak tek bir kafeste barındırılan dişi sıçanlar olmak üzere dört gruba ayrılmıştır. Sıçan yavrularında fiziksel gelişimi takip etmek için ağırlık artışları, yem ve su tüketimleri ölçülürken, mental gelişimlerini takip etmek için Water mase su tankı testi yapılmıştır. Deneyin doğumdan itibaren 4. ayda sonlandırılması planlanmıştır. Gruplar arasında ağırlık artışı, yem ve su tüketimleri karşılaştırıldığında Grup 1 ayrılma stresi yaşayan erkeklerin diğer gruplara göre daha fazla yem ve su tüketimi ile ağırlık artışı sağladığı, grupların mental gelişimleri karşılaştırıldığında, ayrılma stresi yaşayanların toplu halde yaşayanlara göre daha araştırmacı, mücadeleci ve mevcut durumu en iyi şekilde değerlendirdiği tespit edilmiştir. Anneden ayrılma stresi ile birlikte tek tek barındırma, sıçanların fiziksel ve mental gelişmelerini etkiler ve davranışlarında kalıcı değişikliklere neden olabilir. Çalışma sonuçları insanlara genellendiğinde, ailelerin çocuklarına yönelik aşırı koruyucu etkilerinin onların fiziksel ve mental gelişimlerini çok da olumlu etkilemediği, ancak bunun çocukların sokaklara bırakılması anlamına da gelmediği söylenebilir.

REFERENCES

References: 

DESBONNET, L., GARRETT, L., DALY, E., MCDERMOTT, K.W., DİNAN, T.G. (2008). Sexually dimorphic effects of maternal separation stress on corticotrophin-releasing factor and vasopressin systems in the adult rat brain. Int. J. Dev. Neurosci. 26: 259–268.
DESBONNET, L., GARRETT, L., DALY, E., MCDERMOTT, K.W., DINAN, T.G. (2008). Sexually dimorphic effects of maternal separation stress on corticotrophin-releasing factor and vasopressin systems in the adult rat brain. Int. J. Dev. Neurosci. 26: 259–268
ELLENBROEK, B.A., COOLS, A.R. (2000). The long-term effects of maternal deprivation depend on the genetic background. Neuropsychopharmacology. 23 (1): 99-106.
FREEMAN, SCOTT (2002). Biological Science. Prentice Hall; 2nd Pkg edition (December 30, 2004). ISBN 0132187469
GENEST, S.E., GULEMETOVA, R., LAFOREST, S., DROLET G., KINKEAD, R. (2004). Neonatal maternal separation and sex-specific plasticity of the hypoxic ventilatory response in awake rat. J. Physiol. 554: 543–557
GOMEZ, F., HOUSHYAR, H., DALMAN, M.F. (2002). Marked regulatory shifts in gonadal, adrenal, and metabolic system responses to repeated restraint stress occur within a 3-week period in pubertal male rats. Endocrinology. 143 (8): 2852-62.
GÖNENÇ, S., AÇIKGÖZ, O., KAYATEKİN, B.M., UYSAL, N., AKHİSAROĞLU, M. (2000). Effects of footshock stress on superoxide dismutase and glutathione peroxidase enzyme activities and thiobarbituric acid reactive substances levels in the rat prefrontal cortex and striatum. Neurosci Lett. 4, 289 (2): 107-10.
GRABER, J.A., PETERSEN, A.C. (1991). Cognitive Changes in Adolescence: Biological Perspectives. Brain Maturation and Cognitive Development: Comparative and Cross Cultural Perspectives. GIBSON, K.R., PETERSEN, A.C. (eds). United States of America.
1203
F.SEMENDEROĞLU -C. YILMAZ-C.HATİPOĞLU-G. SÖNMEZ-N. GÖKMEN-O. YILMAZ
HE, J., CREWS, F.T. (2007). Neurogenesis decreases during brain maturation from adolescence to adulthood. Pharmacol Biochem Behav. 86 (3): 27–33.
HODGES, H. (1996). Maze Procedures: the radial arm and water maze compared. Cogn Brain Res. 3 (3-4): 167-81
IDE, T. (2003). Laboratuar Hayvanlarında Temel İlkeler. Ankara: Medipress Matbaacılık.
JAKUBOWSKA-DOĞRU, E. (2008). Hayvanlar için davranış testleri, Kognitif Nörobilimler. Bölüm 47. S.Karakaş 8(Ed.). MN Medikal & Nobel Yayın Evi.
KANIT, L., TAŞKIRAN, D., FUREDY, J.J., KULALI, B., MCDONALD, R., POGUN, S. (1998). Nicotine interacts with sex in affecting rat choice between “look-out” and “navigational” cognitive styles in the Morris water maze place learning task. Brain Res. Bull. 46, 5 : 441–445.
KANIT, L., TAŞKIRAN, D.O., YILMAZ, A., BALKAN, B., DEMIRGÖREN, S., FUREDY, J.J., POGUN, S. (2000-a). Sexually dimorphic cognitive style in rats emerges after puberty. Brain Res. Bull. 52, 4: 243–248.
KANIT, L., YILMAZ, O., TAŞKIRAN, D., KULALI, B., FUREDY, J.J., DEMIRGÖREN S., POGUN, S. (2000-b). Sexually dimorphic cognitive style, female sex hormones, and cortical nitric oxide. Physiol. Behav. 71, 3/4: 277–287.
KOSHIBU, K., LEVITT, P., AHRENS, E.T. (2004). Sex-specific, postpuberty changes in mouse brain structures revealed by three-dimensional magnetic resonance microscopy. Neuroimage. 22 (16): 36–45.
LLORENTE, R., GALLARDO, M.L., BERZAL, A.L., PRADA, C., GARCIA-SEGURA, L.M., VIVEROS, M.P. (2009). Early maternal deprivation in rats induces gender-dependent effects on developing hippocampal and cerebellar cells. Int. J. Dev. Neurosci. 27: 233–241
LLORENTE, R., GALLARDO, M.L., BERZAL, A.L., PRADA, C., GARCIA-SEGURA, L.M., VIVEROS, M.P. (2009). Early maternal deprivation in rats induces gender-dependent effects on developing hippocampal and cerebellar cells. Int. J. Dev. Neurosci. 27: 233–241
LLORENTE, R., LLORENTE-BERZAL, A., PETROSINO, S., MARCO, E.M., GUAZA, C., PRADA, C., LÓPEZ-GALLARDO, M., DI MARZO, V., VIVEROS, M.P. (2008). Gender-dependent cellular and biochemical effects of maternal deprivation on the hippocampus of neonatal rats: a possible role for the endocannabinoid system. Dev. Neurobiol. 68: 1334–1347
MARAIS, L., van RENSBURG, S.J., van ZYL, J.M., STEIN, D.J., DANIELS, W.M. (2008). Maternal separation of rat pups increases the risk of developing depressive-like behavior after subsequent chronic stress by altering corticosterone and neurotrophin levels in the hippocampus. Neurosci Res. 61 (1): 106-12.
Anneden Ayrılma ve Tek Tek Barındırıl ma Stresinin Sıçanların
Fiziksel ve Mental Gelişimleri Üzerine Etkileri 1204
MATSUMOTO, Y., YOSHIHARA, T., YAMASAKI, Y. (2006). Maternal deprivation in the early versus late postnatal period differentially affects growth and stress-induced corticosterone responses in adolescent rats. Brain Res. 1115 : 155–161.
MCDONALD, R.J., WHITE, N.M.A. (1993). Triple dissociation of memory systems: hippocampus, amygdala, and dorsal striatum. Behav Neurosci. 107: 3–22. 372
MCCORMICK, C.M., MATHEWS, I.Z. (2009). Adolescent development, hypothalamic– pituitary–adrenal function, and programming of adult learning and memory. Prog Neuro-Psychophar Biol Psychiat
MCCORMICK, C.M., SMITH, C., MATHEWS, I.Z. (2008). Effects of chronic social stress in adolescence on anxiety and neuroendocrine response to mild stress in male and female rats. Behav Brain Res. 5; 187 (2): 228-38. Epub 2007 Sep 14.
MCDONALD, R.J., WHITE, N.M. (1994). Parallel information processing in water maze: evidence for independent memory systems involving dorsal striatum and hippocampus. Behav. Neural Biol. 61: 260–270.
MORRIS, R.G.M. (1981). Spatial localization does not require the presence of local cues. Learn. Motiv. 12: 239–261.
MORRIS, R.G.M. (1981). Spatial localization does not require the presence of local cues. Learn. Motiv. 12: 239–261.
PACKARD, M.G., MC GAUGH, J.L. (1992). Double dissociation of fornix and caudate nucleus lesions on acquisition of two water maze tasks: further evidence for multiplememory systems. Behav Neurosci. 106: 439 – 46. 382
POYRAZ, Ö. (2000). Laboratuar Hayvanları Bilimi. Ankara: Kardelen Matbacılık.
REES, S.L., AKBARI, E., STEINER, M., FLEMING, A.S. (2008). Effects of Early Deprivation and Maternal Separation on Pup-Directed Behavior and HPA Axis Measures in the Juvenile Female Rat. Dev Psychobiol. 50: 315–321.
RICE, D., STAN, B.J. (2000). Critical periods of vulnerability for the developing nervous system: evidence from humans and animal models, Environ. Health Perspect. 108: 511–533.
ROMEO, R.D., KARATSOREOS, I.N., MCEWEN, B.S. (2006). Pubertal maturation and time of day differentially affect behavioral and neuroendocrine responses following an acute stressor. Horm Beh . 50 (3): 463-8.
SAUGSTAD, L.F. (1998). Cerebral lateralisation and rate of maturation. Int. J. Psychophysiol. 28: 37–62.
SCHMIDT, M.V., LEVINE, S., ALAM, S., HARBICH, D., STERLEMANN, V., GANEA, K., DE KLOET, E.R., HOLSBOER, F., MULLER, M.B. (2006). Metabolic signals modulate hypothalamic–pituitary–adrenal axis activation during maternal separation of the neonatal mouse. J. Neuroendocrinol. 18 : 865–874.
1205
F.SEMENDEROĞLU -C. YILMAZ-C.HATİPOĞLU-G. SÖNMEZ-N. GÖKMEN-O. YILMAZ
SPEAR, L.P. (2000). The adolescent brain and age related behavioral manifestations. Neurosci Biobehav Rev. 24 (4): 417-63
TUGYAN, K., UYSAL, N., ÖZDEMİR, D., SÖNMEZ, U., PEKÇETİN, C., ERBİL, G., SÖNMEZ, A. (2006). Protective effect of melatonin against maternal deprivation-induced acute hippocampal damage in infant rats. Neurosci Lett. 1; 398 (1-2): 145-50.
UYSAL, N., ACIKGÖZ, O., GÖNENÇ, S., KAYATEKİN, B.M., KIRAY, M., SÖNMEZ, A., SEMİN, I. (2005). Effects of acute footshock stres on oxidant enzyme activities in the adolscent rat brain. Physiol Res. 54 (4): 437-42.
UYSAL, N., GÖNENÇ, S., AÇIKGÖZ, O., PEKÇETİN, C., KAYATEKİN, B.M., SÖNMEZ, A., SEMİN, I. (2005). Age-dependent effects of maternal deprivation on oxidative stress in infant rat brain. Neurosci Lett. 12-19; 384 (1-2): 98-101.
UYSAL, N., ŞİŞMAN, A.R., GÖNENC, S., AÇIKGÖZ, O., KAYATEKİN, B.M., YALAZ, G. (2008). Effects of Repeated Maternal Separation On Oxidative Stress In Adolescent Male and Female Rat Brains. Journal of Neurological Sciences (Turkish). 25 (3): 155-162
UZBAY, T. (2004). Psikofarmakolojinin Temelleri ve Deneysel Araştırma Teknikleri. Çizgi Tıp Kitabevi.
VIVEROS, M.P., LLORENTE, R., DÍAZ, F., ROMERO-ZERBO, S.Y., BERMUDEZ-SILVA, F.J., RODRÍGUEZ DE FONSECA, F., ARGENTE, J., CHOWEN, J.A. (2010). Maternal deprivation has sexually dimorphic long-term effects on hypothalamic cell-turnover, body weight and circulating hormone levels. Horm Behav. 58 (5): 808-19.
VIVEROS, M.P., LLORENTE, R., LÓPEZ-GALLARDO, M., SUAREZ, J., BERMÚDEZ-SILVA, F., DE LA FUENTE, M., RODRÍGUEZ DE FONSECA F., GARCÍA-SEGURA, L.M. (2009). Sex-dependent alterations in response to maternal deprivation in rats. Psychoneuroendocrinology. 34 (Suppl1) : 217–226.
WAGNER, B.M., COMPAS, B.E. (1990). Gender, instrumentality, and expressivity: moderators of the relation between stress and psychological symptoms during adolescence. Am J Community Psychol. 18 (3): 383-406.
YILDIRIM, M., MAPP, O.M., JANSSEN, W.G.M., YIN, W.L., MORRISON, J.H., GORE, A.C. (2008). Postpubertal decrease in hippocampal dendritic spines of female rats. Exp Neurol. 210 (3): 39–48.

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