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Verticillium dahliae ile İnokule Edilen Yonca Bitkisinden (Medicago sativa cv. Vertus) Elde Edilen Fenilalanin Amonia Lyaz Enziminin Kademeli Olarak Saflaştırılması

Stepwise Purification of Phenylalanine Ammonia Lyase (PAL) Enzyme Obtained from Lucerne (Medicago sativa cv. Vertus) Inoculated with Verticillium dahliae

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Abstract (2. Language): 
An increased activity in phenylalanine ammonia lyase (EC 4.3.1.24, PAL) enzyme in lucerne seedlings (Medicago sativa L. cv. Vertus) following inoculation with Verticillium dahliae was measured after 48 h of inoculation. Purifications of PAL with 0-50% (NH 4 ) 2 SO 4 precipitation and Sephadex G-100 gel filtration resulted in 1.75 and 1.97 fold increases with 75 and 60 % recoveries, respectively. This study showed that the purification of the enzyme clearly resulted in significant differences in respect to activities between fractions. This property could be important for further characterization of pathogen-related proteins.
Abstract (Original Language): 
Verticillium dahliae ile inokule edilen yonca fidelerinden (Medicago sativa L. cv. Vertus ) elde edilen fenilalanin amonia lyaz (EC 4.3.1.24, PAL) enzim aktivitesindeki artış inokulasyondan 48 saat sonra ölçülmüştür. PAL enziminin % 0-50 (NH 4 ) 2 SO 4 ve Sephadex G-100 jel filtrasyon yöntemi ile saflaştırılması, %75 ve %60 lık geri kazanım ve 1.75 ve 1.97 lik bir artışa yol açmıştır. Enzim saflaştırması fraksiyonlar arasında aktivite açısından önemli farklılıkların ortaya çıkmasına neden olmuştur. Bu özellik patojen ile ilgili proteinlerin detaylı karakterizasyonu için önemlidir.

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