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Arpa Mikrosatelitlerinin Ekmeklik Buğdaydaki Genetik Çalışmalar İçin Kullanım Olanaklarının Araştırılması

Evaluation of Barley Microsatellites for Genetic Studies in Bread Wheat

Journal Name:

  • Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi

Publication Year:

  • 2003

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Genome related studies have been progressed slowly in most plants due to their large genome size and complex genome structure. Therefore it is becoming important to evaluate applicability of results belonging to certain species for other species that can be genetically linked. In this study, DNA from a bread wheat variety ‘Gerek-79’, growing in wide area of Turkey, was amplified with 29 barley microsatellite primers by PCR. Allele sizes of radioactively labeled PCR products were compared with those obtained with same primers in barley. Based on allele sizes, microsatellites producing products at similar length for wheat were determined as HVM15, HVM20, HVM23, HVM26, HVM31, HVM36, HVM40, HVM44, HVM54 and HVM62. In future studies, level of polymorphism among different wheat varieties will be determined based on those microsatellites from this study and possibility of using them for genotyping studies being addressed.
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Abstract (Original Language): 
Genomik çalışmalar çoğu bitkinin oldukça büyük genoma sahip olmaları ve kompleks genom yapısı göstermeleri nedeniyle yavaş ilerleme göstermektedir. Bu nedenle bir bitki türünden elde edilen araştırma sonuçlarının o tür ile genetik ilişkisi olabilecek bir başka tür üzerinde uygulanabilirliğinin araştırılması önem kazanmaktadır. Bu araştırmada, Türkiye’de fazla miktarda yetiştirilen bir ekmeklik buğday çeşidi olan ‘Gerek-79’’un DNA’sı 29 arpa mikrosatelitinin her birine özgü polimeraz zincir reaksiyonu (PCR) primerleri kullanılarak çoğaltılmıştır. Buğdaydan elde edilen radyoaktif PCR ürünleri, daha önce arpada bu primerlerin kullanılması sonucu elde edilmesi muhtemel ürünlerin alel uzunlukları ile karşılaştırılmıştır. Alel uzunluklarına göre buğdayda benzer uzunlukta amplifikasyon ürünleri oluşturan mikrosatelitler HVM15, HVM20, HVM23, HVM26, HVM31, HVM36, HVM40, HVM44, HVM54 ve HVM62 olarak saptanmıştır. Bundan sonraki çalışmalarda belirlenen mikrosatelitlerin farklı buğday çeşitlerindeki polimorfizm özellikleri saptanarak, buğday genotiplendirme çalışmalarında kullanılma olasılığı incelenecektir.
FULL TEXT (PDF): 
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  • 2
34
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  • Turkish

REFERENCES

References: 

Akkaya, M.S., A.A Bhagwat, P.B. Cregan, 1992. Length Polymorphisms of Simple
Sequence Repeats DNA in Soybean. Genetics, 132:1131-1139
Ayres, N.M., A.M. McClung, P.D. Larkin, H.F.J. Bligh, C.A. Jones, W.D. Park,
1997. Microsatellite and a Single Nucleotide Polymorphism Differentiate
Apparent Amylose Classes in an Extended Pedigree of US Rice Germplasm.
Theoretical and Applied Genetics, 94:773-781
Brown, S.M., M.S. Hopkins, S.E. Mitchell, M.L. Senior, T.Y. Wang, R.R. Duncan,
F. Gonzales-Candelas, S. Kresovich, 1996. Multiple Methods for the
Identification Polymorphic Simple Sequence Repeats (SSRs) in Sorghum
[Sorghum bicolor (L.) Moench]. Theoretical and Applied Genetics, 93:190-198
Bolstein, D., R.L. White, M. Skolnick, R.W. Davis, 1980. Construction of a Genetic
Linkage Map in Man Using Restriction Fragment Length Polymorphism.
American Journal Human Genetics, 32:314-331
Büyükünal Bal, E.B., M.S. Akkaya, 2002. Assessment of Two Highly Polymorphic
Barley Microsatellite Markers for Detecting Polymorphism in Wheat. Turkish
Journal of Biology, 26:9-12
Cipriani, G., G. Lot, W.G. Huang, M.T. Marrazzo, E. Peterlunger, R. Testolin,
1999. AC/GT and AG/CT Microsatellite Repeats in Peach [Prunus persica (L)
Batsch]: Isolation, Characterization and Cross-species Amplification in Prunus.
Theoretical and Applied Genetics, 99:65-72
Devos, K.M., T. Millan, M.D. Gale, 1993. Comparative RFLP Maps of the
Homoeologous group-2 Chromosomes of Wheat, Rye, and Barley. Theoretical
and Applied Genetics, 85:784-792
Akkaya, M.S., A.A Bhagwat, P.B. Cregan, 1992. Length Polymorphisms of Simple
Sequence Repeats DNA in Soybean. Genetics, 132:1131-1139
Ayres, N.M., A.M. McClung, P.D. Larkin, H.F.J. Bligh, C.A. Jones, W.D. Park,
1997. Microsatellite and a Single Nucleotide Polymorphism Differentiate
Apparent Amylose Classes in an Extended Pedigree of US Rice Germplasm.
Theoretical and Applied Genetics, 94:773-781
Brown, S.M., M.S. Hopkins, S.E. Mitchell, M.L. Senior, T.Y. Wang, R.R. Duncan,
F. Gonzales-Candelas, S. Kresovich, 1996. Multiple Methods for the
Identification Polymorphic Simple Sequence Repeats (SSRs) in Sorghum
[Sorghum bicolor (L.) Moench]. Theoretical and Applied Genetics, 93:190-198
Bolstein, D., R.L. White, M. Skolnick, R.W. Davis, 1980. Construction of a Genetic
Linkage Map in Man Using Restriction Fragment Length Polymorphism.
American Journal Human Genetics, 32:314-331
Büyükünal Bal, E.B., M.S. Akkaya, 2002. Assessment of Two Highly Polymorphic
Barley Microsatellite Markers for Detecting Polymorphism in Wheat. Turkish
Journal of Biology, 26:9-12
Cipriani, G., G. Lot, W.G. Huang, M.T. Marrazzo, E. Peterlunger, R. Testolin,
1999. AC/GT and AG/CT Microsatellite Repeats in Peach [Prunus persica (L)
Batsch]: Isolation, Characterization and Cross-species Amplification in Prunus.
Theoretical and Applied Genetics, 99:65-72
Devos, K.M., T. Millan, M.D. Gale, 1993. Comparative RFLP Maps of the
Homoeologous group-2 Chromosomes of Wheat, Rye, and Barley. Theoretical
and Applied Genetics, 85:784-792

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