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Lilium candidum L.’nin MİKROÇOĞALTIMINDA BAZI ANTİBİYOTİK ve FUNGUSİT UYGULAMALARININ ETKİSİ

THE EFFECT OF SOME ANTIBIOTIC AND FUNGUCIDE APPLICATIONS ON THE MICROPROPAGATION OF Lilium candidum L.

Journal Name:

  • Mugla Journal of Science and Technology

Publication Year:

  • 2017
DOI: 
10.22531/muglajsci.307105

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Lilium candidum is one of the important commercial crop worldwide. In this study, a protocol for micropropagation of Lilium candidum was developed the effect of some antibiotic and fungicide applications and also the effect of the light. In experiments, bulb scales of Lilium candidum L. were used. The plant material was sterilized superficially and it was dipped in solutions which contain antibiotic and fungicide 30 minutes was cut into pieces and cultured in Murashige and Skoog (1962) medium supplemented with 0.1 mg/L NAA + 0.01 mg/L BA, 30 mg/L sucrose and 8 mg/L agar. The application of antibiotic and fungicide was the application of Streptomycin + Penicillin, Streptomycin + Benomyl and Benomyl + Nystatin in every two different doses. Bulb scales were cutting into lateral as 5-10 mm pieces. The explants were cultured 16 hrs light - 8 hrs dark photoperiodic condition; the light was 1600 lux, and also were cultured in completely darkness condition in the same applications. Culture room temperature was between 19° and 22 °C. Bulblet formation (%) was observed in photoperiodic condition higher than darkness one. In the Benomyl + Nystatin solution bulb formation (%) was the highest (97.4%). In addition the best application was obtained Benomyl + Nystatin solution both in respect of sterilization and the point of view the number of the bulblets.
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Abstract (Original Language): 
Bu çalışmada, dünya çapında ticari öneme sahip olan Lilium candidum L.’nin mikroçoğaltımında bazı antibiyotik ve fungusit uygulamalarının in vitro’da ki etkisi, uygun sterilizasyon ve ışığın etkisi araştırılmıştır. Denemelerde temel MS (1962) ortamı kullanılmış, ortama 0.1 mg l/L NAA+ 0.01 mg l/ L BA, 30 g /L şeker ve 8 g /L agar ilavesi yapılmıştır. Bitki materyali (soğan pulları) yüzeysel sterilizasyonu takiben antibiyotik ve fungusit içeren solusyonlarda 30 dk bekletildikten sonra parçalara ayrılarak kültüre alınmıştır. Antibiyotik ve fungusit uygulamaları: iki farklı dozda Streptomycin + Penicilin uygulamaları, iki farklı dozda Streptomycin + Benomyl uygulamaları ve iki farklı dozda Benomyl + Nystatin uygulamalarıdır. Lilium candidum soğan pulları 5-10 mm boyutunda enine parçalara ayrılarak kültüre alınmıştır. Karanlık ve fotoperiyodik koşulda (16 saat aydınlık- 8 saat karanlık) kültüre alınan eksplantlarda % soğancık oluşumunun, fotoperiyodik koşulda karanlığa göre daha yüksek olduğu gözlenmiştir. Ayrıca yüzeysel sterilizasyondan sonra yapılan uygulamalarda hem sterilizasyon yönünden hem de eksplant başına elde edilen soğancık sayısı bakımından en olumlu uygulamanın Benomyl + Nystatin solusyonlarının olduğu belirlenmiştir.
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Filiz Altan, Betül Bürün
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