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SIÇANLARDA NORMOBARIK HIPOKSI ANTRENMANLARININ ELEKTRİK UYARISI İLE OLUŞTURULAN İSKELET KAS YORGUNLUĞUNA ETKİSİ

THE EFFECT OF NORMOBARIC HYPOXIC TRAINING ON ELECTRICAL STIMULATION INDUCED SKELETAL MUSCLE FATIGUE IN RATS

Journal Name:

  • Pamukkale Spor Bilimleri Dergisi

Publication Year:

  • 2013

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: The aim of this study was to investigate the effect of normobaric hypoxic training (altitude training) on skeletal muscle fatigue and peak force induced by electrical stimulation in rats. Material and Method: A total of 22 Sprague Dawley male rats were randomly divided into 4 groups: Control (LL, n=5), live low-train low (LLTL, n=6), live hypoxia-train hypoxia (LHTH, n=5), live hypoxia-train low (LHTL, n=6). LHTH groups, were exposed to hypoxia for 4 weeks. LHTL, were exposed to 12 hours hypoxia and 12 hours normoxia a day for 4 week. Hypoxia is maintained by reducing 15% O2 level (8850 SUMMIT, Altitude Tech, Canada). The training protocol corresponded to 60-70 % of maximal exercise capacity by means of maximal exercise test. This test consisted of graded exercise on treadmill, with increments of 0.3 km/h for LHTH group,and 0.6 km/h LLTL and LHTL groups, every 3 min, starting with 0.3 km/h up to maximal intensity attained for each rat. Rats in the training groups ran on the rat treadmill (MAY-TME 9805, Commat, Ankara, Turkey) for 20-35 min a day, 4 day a week for 4 week. At the end of the experimental period, maximal exercise test was repeated at 2nd day of low altitude. For peak force and muscular fatigue, the electrical stimulation was administered with an intensity of 6-7 V and pulses of 2 msec duration. To induce a tetanic contraction, the frequency was raised from 0.2 to 60 Hz and kept there until the muscular force fell to 50% of its value at the onset of that particular contraction. Kruskal Wallis and Mann-Whitney U tests were used for the differences between groups, p values <0.05 accepted as statistically significant. Results and Conclusion: As a result of study, LLTL and LHTL groups peak force are more high than LL groups (p=0.004; p=0.0017) also there is a significant difference between LLTL and LHTH groups (p=0.017). The muscular fatigue values of LHTH is lower than LLTL and LHTL, but these values did not reach a significant difference. As a result of this study, LHTH group is more disadvantaged than other training groups.
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Abstract (Original Language): 
Gereç ve Yöntem: Toplam 22 adet erkek Sprague Dawley cinsi sıçan rastgele 4 gruba ayrıldı: Kontrol (LL, n=5), düşük yükseltide (normoksi) yaşam ve antrenman (LLTL, n=6), hipokside yaşam ve hipokside antrenman (LHTH, n=5), hipokside yaşam ve düşük yükseltide antrenman (LHTL, n=6). LHTH grubu 4 hafta sürekli hipokside kalırken, LHTL grubu 4 hafta boyunca 12 saat hipoksik, 12 saat normoksik ortamda barındırıldı. Hipoksi O2 seviyesinin %15’e düşürülmesi ile sağlandı (8850 SUMMIT, Altitude Tech., Kanada). Antrenman protokolü, maksimal egzersiz testi sonucunda, maksimal egzersiz kapasitesinin %60-70’ine denk gelen şiddette uygulandı. Kullanılan egzersiz testi basamaklı egzersiz testi olup, LHTH grubu için 0.3 km/s diğer gruplar için 0.6 km/s başlangıç hızında ve her 3 dakikada 0.3 km/s hız artışı sağlanarak %0 eğimde sıçanın koşu bandında (MAY-TME 9805, Commat, Ankara,Türkiye) koşu konumunu koruyamadığı noktaya kadar devam ettirildi. Maksimal egzersiz kapasitesinin % değeri egzersiz şiddetinin tespitinde kullanılarak her sıçan günde 20-35 dk, haftada 4 gün ve 4 hafta boyunca egzersize tabi tutuldu. Antrenman periyodu bitiminin 2. günü düşük yükseltide maksimal egzersiz testi tekrarlandı. Egzersiz testi sonrası siyatik sinire verilen elektriksel uyarı (0.2-60Hz, 6V, 2msn) ile gastrokinemius kasında tetanik kontraksiyon oluşturularak zirve kuvvet ve yorgunluk verileri elde edildi. Çalışmada elde edilen veriler Kruskal-Wallis ve Man Whitney U testi kullanılarak, 0.05 anlamlılık düzeyinde değerlendirildi. Sonuçlar: Elde edilen zirve kuvvet değerleri açısından, LLTL ve LHTL grupları LL grubuna göre daha yüksek zirve kuvvet değerine sahip iken (p=0.004; p=0.0017) LLTL grubu ile LHTH grubu arasında fark istatistiksel olarak anlamlıdır (p=0.017). Buna karşın kassal yorgunluk değerleri arasında istatistiksel olarak anlamlı bir farklılık saptanmamıştır. Bu sonuçlar LHTH grubunun diğer gruplara göre daha dezavantajlı olduğunu göstermektedir.
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