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Tavşanlarda oluşturulan deneysel sepsis modelinde düşük doz n-asetilsistein tedavisinin etkinliği

Efficacy oflow dose n-acetylcystein theraphy on experimental rabbit sepsis model

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Abstract (2. Language): 
Purpose: To determine the effect of NAC therapy on plasma levels. the protectıve role of tissue ınjury and organ dys-functıon of free oxygen radicals in experimental sepsis model. Material and method: 30 female rabbits were divided into 3 groups. each composed of 10 rabbits. Sepsis was constituted by caeucum lıgation perforation tecniçue. Groups named sham, sepsis and sepsis + NAC (lOmg/kg/dayj were formed and medication was given I2and 24 hours after caeucum lıgation and perforation. At the same time blood samples to measure blood gases, plasma glutathıonfGSH}. malondialdehyde/MDA} and biochemical diagnosis were collected. Tissue samples were obtained. Findings: Plasma GSH levels were elevated in NAC group with respect to sepsis group/P<0.05}. Plasma MDA levels were decreased in sepsis group when compared to NAC group(P<0.05}. Liver tissue MDA levels were found to be same ın both groups. AST, ALT and creatınine values vvere elevated signifıcantly ın NAC group when compared to sepsis groupfP<0.05}. Apoptosis in lıver tissue was signifıcantly decreased ın NAC group when compared to sepsis group(P<0.05}. Degree of mononuclear infıltration İn kidneys were decreased in NAC group when compared to sepsis group(P<0.05). Conclusion: Application oflovv dose NAC. an antıoxidative agent, in experimental sepsis model has beneficıal effects on pulmonary functions and plasma MDA GSH levels. More and comprehensive studies vvhich tests different NAC doses are needed to determine the role for NAC in experimental sepsis model.
Abstract (Original Language): 
Anaç: Deneysel sepsis modelinde N-asetılsisteın'ın (NAC) serbest oksijen radikalleri ve plazma düzeylerine olan etki¬lerini, organ fonksiyon bozuklukları ve doku hasarını önlemedeki rolünü belirlemektir. Materyal ve metod: 30 dişi tavşan 1CVarlı gruplara ayrıldılar. Tavşanlarda çekum ligasyon perforasyon (ÇLPj yöntemiyle sepsis oluşturuldu. Sham grubu, sepsis grubu ve sepsis + NACflO mg/kg/günj şeklinde gruplar oluşturulup, ilaçları CLP den hemen sonra, I2.ve24. saatte verildi. Aynı saatlerde kan gazları, plazma glutatyonfGSH}, malondialdehidfMDA}, biyokimyasal tetkik için kan örnekleri alindi- Doku örnekleri alındı. Bulgular: Sepsis grubuyla kıyaslandığında NAC grubunda plazma OSH artmış (P<0.05} ve MDA değerleri azalmış bulundu (P<0.05 }. NAC grubunda SGOT. SGPT ve kreatinin değerlen sep¬sis grubuna göre artmış bulundu (P<0.05}. NAC grubunun arteriyel kan P02 ve Sa02 değerleri sepsis grubuna göre artmış bulundu (P<0.05}. NAC grubunda karaciğerin histopatolojik incelemesinde apopitoz derecesi incelemesinde sepsis grubuna göre anlamlı düzeyde azalmıştı (P<0.05}. NAC grubunun böbrekteki mononükleer hücre ınfiltrasyonu derecesi sepsis grubuna göre anlamlı düzeyde azalmış olarak bulundu (P<0.05}. Sonuç: Deneysel sepsis modelinde antioksidan bir ajan olan NAC'in düşük doz uygulanmasında plazma MDA GSH düzeylerine ve akciğer fonksiyonlarına olumlu etkileri mevcuttur. Daha kapsamlı ve yeni çalışmalarla NAC'in değişik dozlarda verilmesiyle sepsis tedavisinde NAC tedavisinin önemi daha iyi anlaşılacaktır.

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