You are here

Home » Content

http://www.selcuktipdergisi.org/arsiv/gecmisarsivler/1321344909.pdf

Reevaluation of Candida albicans strains tor the Identification of Candida dubliniensis

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2005

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
The aim of this study was to fınd out vvhether there is Candida dubliniensis strain in the clinıcal preperatıons from Celal Bayar University Faculty of Medıcine Hospital Clinica! Mycology Laboratories identified as Candida albicans strains and to determine the rcliabiiity of the CHROMagar Candida medium for Identification of the species. İn our study the evaluation of 140 isotated strains of Candida albicans was performed retrospectively according to the grovvth profıles obtained byAPI 20CAUX, CHROMagar Candida, methyl-blue Sabouraud dextrose agar. rice flour Tween-80 agar and Sabouraud dextrose agar at 42 °C and 45 °C Two (1.4 %} of 140 strains were identified as Candida dubliniensis while 138 (98.6 %) were identified as Candida albicans. İt is concluded that combined use of CHROMagar Candida and Sabouraud dextrose agar at 45 °C in the diferentia-tion of Candida albicans and Candida dubliniensis strains is convenient ın the routine diagnosis based in ıts specifıcı-ty, cost-effectiveness and simplicity.
Bookmark/Search this post with
Abstract (Original Language): 
Bu çalışmanın amacı Celal Bayar Üniversitesi Tıp Fakültesi Hastanesi Klinik Mikoloji laboratuvarına gönderilen klinik örneklerden infeksıyon etkeni olarak tanımlanan Candida albicans suşları arasında Candida dubliniensis susunun olup olmadığını araştırmak ve tür belirlenmesinde CHROMagar Candida besiyerınin güvenirliliğim saptamaktır. Çalışmamızda Candida albicans olarak izole ettiğimiz 140 susun retrospektif olarak değerlendirilmesi: API 20C AUX. CHROMagar Candida, metil blue-Sabouraud dextroz agar. pirinçunu Tween-80 agar. 42 °C ve 45 °C de Sabouraud dextroz agar besiyerlerinde üreme durumlarına göre yapılmıştır. Yüzkırk adet susun ikisi 1% 1.4} Candida dubliniensis. 138'i (% 98-6) Candida albicans olarak tanımlanmıştır Sonuç olarak Candida albicans ve Candida dubliniensis' in tür ayrımında CHROMagar Candida ve 45 "C Sabouraud dextroz agar yöntemlerinin birlikte kullanılmasının tanı özgüllüğü, maliyet ucuzluğu ve uygulama kolaylıklarından dolayı rutin tanıda kullanılmasının uygun olacağı kanısına varılmıştır
FULL TEXT (PDF): 
PDF icon 1321344909.pdf.pdf
  • 2
35-40
  • Turkish

REFERENCES

References: 

1. Mycology İn; Koneman EW, Ailen SD, Dovvell VR, Janda WM, Sommers HM, editors. Color Atlas And Textbook of Diagnoric Microbiology. Philadelphia 5th Ed. JB üppincotî Company, 1998: p.573- 636.
2. Yücel A, Kantarcıoğlu AS. Candıda'ların patojenlik belirti¬len. Cerrahpaşa Tıp Derg 2000:31 (3): 172-86.
3. Sullivan DJ. Moran GP, Pinjon E, Al-Mosaid A Stokes C. Vaughan C. et al. Comparison of the epidemiology, drug resistance mechanisms. and virulence of Candida dubli¬niensis and Candida albicans. FEMS Yeast Res. 2004; 4(4-5):369-76
4. Sayıner AA Özbakkaloglu B, Hiimioglu S, İnci R Geriyatrik Yaş Grubunda Mukozal Maya Kolonizasyonu. infeksıyon Derg 1997; 1 1(2); 137-40.
5. Maenza RJ. Merz WG. Candida albicans and Related Species. İn: Gorbach S!.. Bartlett NR, Blacklow NR, edi-tors infectious Diseases. 2th Ed. 1998: 2313-21.
6. Sullivan D., Colernan D. Candida dubliniensis :Characreristics and Identification. J Clin Microbiol 1998; 36(2): 329-4.
7. Hiimioglu S. Aydemir Ş. İnci R Gürel SÖ. Tümbay E. Türkiye'de ilkkez izole edilen Candida dubliniensis kökeni. İnfeksiyon Derg 1998; 12(4) : 545-8.
8. Coleman DC, Sullivan DJ, Mossman JM. Candida dubli¬niensis. J Clin Microbiol 1997; 35(1 1): 301 1-2.
9. Pinjon E, Sullivan D, Salkın I, Shanley D. Coleman D. Simple, mexpensive, reliable method for differentiation of Candida dubliniensis from Candida albicans. J Cim Microbiol 1 998; 36(7):2093-5.
10. Meis JF, Ruhnke M, De Pauvv BE, Odds FC, Siegert W, Vervveij PE. Candida dubliniensis candidemia in patients with chemotherapy-induced neutrcpenia and bone mar-row transplatıon. Emerg Infect Dis 1999; 5(1]: 155-8
1 İ. Yücel A Kantarcıogiu AS. Candida albicans kökenlerinin kaç tanesi Candida dubliniensis? 1. Ulusal Mantar Hastalıkları ve Klinik Mikoloji Kongresi Kitabı; 4-6 Mayıs 1999; İzmir, Türkiye, s. 263-4.
12. Tintelnot K, Haase G. Seibold M. Bergmann F, Staemmler M. Franz T, et al. Evaluation of phenotypic markers for selection and Identification of Candida dubliniensis. J Clin Microbiol 2000; 38(4): 1599-608.
13. Sullivan D, Coleman D. Candida dubliniensis: an emerg-ıng opportunıstic pathogen. Curr Top Med Mycol 1 997; 8(1-2): 15-25.
i 4. Kurzai O. Kortıng HC, Harmsen D, Bautsch W. Molitor M. Frosch M, et al. Molecular and phenotypic identification of the yeast pathogen Candida dubliniensis. J Mol Med 2000; 78(9): 521-9.
15. Odds FC, Van Nuffel L, Dams G. Prevalence of Candida dubliniensis isolates in a yeast stock collection. J Clin Microbiol 1998; 36(10): 2869-73.
16. Polacheck 1. Strahilevitz J, Sullivan D, Donnelly S, Salkin IF. Coleman DC. Recovery of Candida dubliniensis from Non-Human Immunodefiency vırus-infected patients iri Israel J Clin Microbiol 2000; 38(1): 170-4.
1 7. Jabra-Rizk MA, Baqui AA, Keiley Jl. Falkler WA Jr, Merz
WG, MeillerTF. Identification of Candida dubliniensis in a prospective study of patients in the United States. J Clin Microbiol 1999; 37(2): 321-6.
18. Jabra-Rizk MA Falkler VVA Merz WG, Bagui AA Kelley Jl, MeillerTF. Retrospective idetification and characterizatıon of Candida dubliniensis isolates among Candida albicans clinical laboratory isolates from Human Immunodeficiency Virüs (HlV)-infected and nön- HİV infected individuals. J Clin Microbiol 2000;38(6): 2423-6
19. Kantarcıogiu AS, Yücel A. Reidentıfıcatıon of Candida dubliniensis İsolates among Candida albicans Strains Isolated from Debilitated or Immunocompromised Patients in a Turkish University Hospital. Clin Microbiol Infect 2001; 7fSuppl I): 336-7.
20. Hiimioglu S, Aydemir Ş. inci R, Gürel OS, Tümbay E. Candida albicans kökenlerinin Candida dubliniensis için yeniden değerlendirilmesi. 1. Ulusal Mantar Hastalıkları ve Klinik Mikoloji Kongresi kitabı; 4-6 Mayıs 1999; izmir, Türkiye, s. 265-6.
2 I. Af ikan S, Darka O, Hasceiik G, Günalp A Identification of
Candida dubliniensis strains using heat toierance tests. morphologıcal charactenstics and molecular methods Mikrobiyol Bült 2003;37[ I):49-7.
22. Gales AC, PfcNer MA, Houston AK, Joly S, Sullivan DJ. Coleman DC, et al Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and Ct— Methyl-d-Glucoside as Determined with the API 20 CAUX and Vitek YBC Systems. J Clin Microbiol 1999; 37(12): 3804-8.
23. Pıncus DH. Coleman DC, Pruitt WR, Padhye AA. Salkın IF, Geimer M. et al. Rapid Identification of Candida dublin¬iensis vvith Commercial Yeast Identification Systems. J Clin Microbiol 1999, 37(1 1): 3533-9.

Thank you for copying data from http://www.arastirmax.com