You are here

Home » Content

Mesane kanseri ile DNA tamir genlerinden KU 70/80 genetik varyantlarının ilişkisinin araştırılması

Investigation of the relation between KU 70/80 DNA repair gene genetic variants and bladder cancer

Journal Name:

  • Smyrna Tıp Dergisi

Publication Year:

  • 2016

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: Bladder cancer is the 7th most common cancer among men 17th most common among women and 9th most common in general around the world. Several studies focus on the DNA repair mechanism and DNA repair genes, which is one of the fundamental steps of cell division process. Investigation of the relationship between bladder cancer and genetic variants of KU 70/80 DNA repair genes is the main objective of the study.. Materials and Methods: Breaking of phosphodiester bonds in both chains of DNA causes DNA double strand breaks. The two major ways of double chain repair mecanisims are homologus recombination (HR) and Non-Homolog end Joining (NHEJ). Ku is a heterodimeric protein expressed by XRCC gene family and consist of two subunits as Ku70 and Ku80, which plays role in NHEJ repair mechanism. The study group consisted of 38 bladder cancer patients, aged 30¬60, 34 males and 4 females, followed up and treated at Isparta Süleyman Demirel Univer sity Research and Practice Hospital, Urology Service between 2010-2011. Ku70 and Ku80 genotyping studies were carried out and statistical significance was investigated. Results: In terms of the data obtained in the study, it was found that there was no statistically significant relationship between the Ku70 -1310C/G and Ku80 -1401G/T polymorphisms studied for the first time in the study population and bladder cancer. Discussion: Results are scientificly important because of the elimination of two factors which can play role in bladder cancer.
Bookmark/Search this post with
Abstract (Original Language): 
Amaç: Mesane kanseri, dünya genelinde en sık görülen kanser türleri arasında erkeklerde 7. kadınlarda 17. genel olarak bakıldığında 9. sıradadır. Mesane kanseri ile ilgili yapılmış birçok çalışmada hücre bölünme olayında temel basamaklardan biri olan DNA tamir mekanizması ve bu mekanizmanın işleyişini sağlayan DNA tamir genleri üzerinde yoğunlaşmıştır. Mesane kanseri ile DNA tamir genlerinden KU 70/80 genetik varyantlarının ilişkisinin araştırılması çalışmanın ana amacıdır. Gereç ve Yöntem: DNA çift zincir kırıkları, DNA sarmalının her iki zincirinde yer alan fosfodiester bağının parçalanması sonucunda meydana gelir. Ökaryotik hücrelerde, DNA çift zincir tamir mekanizmasında homolog rekombinasyon (HR) ve serbest uçların homolog olmayan şekilde bağlanması (Non-Homolog end Joining, NHEJ) olmak üzere iki major yol vardır. NHEJ tamir mekanizmalarında görev alan Ku, XRCC gen ailesi tarafından ifade edilen Ku70 ve Ku 80 olmak üzere iki alt birimden oluşan heterodimerik bir proteindir. Çalışma grubu, 2010-2011 yılları arasında Isparta Süleyman Demirel Üniversitesi Araştırma ve Uygulama Hastanesi, Üroloji Servisinde takip ve tedavi edilen, yaşları 30- 60 arasında, 34 erkek ve 4 kadın, toplam 38 mesane kanserli hastadan oluşmaktadır. Ku70 ve Ku80 genotiplendirme çalışması yapılarak, istatistiksel anlamlılık araştırılmıştır. Buılgular: Araştırmada elde edilen veriler doğrultusunda, araştırma popülasyonunda ilk kez çalışılan Ku70 -1310C/G ve Ku80 -1401G/T polimorfizmleri ile mesane kanseri arasında istatistiksel anlamlı bir ilişki olmadığı tespit edilmiştir. Sonuç: Bulgular mesane kanseri oluşumunda rolü olabilecek bir etkeni elediğinden bilimsel açıdan önemlidir.
FULL TEXT (PDF): 
PDF icon 80-genetik-varyantlarinin-iliskisinin-arastirilmasi.pdf
  • 1
27
32
  • Turkish

REFERENCES

References: 

1.
İmyanitov E, Hanson K, Zhivotovsky B. Polymorphic variations in apoptotic genes and Cancer predisposition. Cell Death Differ 2005;12(8):1004-7.
2. Pierce AJ, Hu P, Han M, Ellis N, Jasin M. Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells. Genes Dev
2001;15(24):3237-42.
Smyrna Tıp Dergisi -32¬3. Thacker J, Zdzienicka MZ. The XRCC genes: expanding roles in DNA double-strand break
repair. DNA Repair (Amst) 2004 ;3(8):1081-90.
4. Fell VL- Schild Poulter C. The Ku heterodimer: function in DNA repair and beyond. Mutat Res
Rev Mutat Res 2015;76:15-29.
5. Jones JM, Gellert M, Yang W. A Ku bridge over broken DNA. Structure 2001;9(10):881-4.
6. Dik C. van Gent, Jan HJ, Kanaar H, Kanaar R. Chromosomal stability and the DNA double-stranded break connection. Nature Reviews
Genetics 2001;2:196-206.
7. Goode EL, Ulrich CM, Potter JD.
Polymorphisms in DNA repair genes and associations with cancer risk. Cancer Epidemiol
Biomarkers Prev 2002;11(12):1513-30.
8. WHO cancer mortality database. http://www-dep.iarc.fr/ accessed on:05.01.2015
9. Colombel M, Soloway M, Akaza H, Bohle A, Palou J, Buckley et al. Epidemiology, Staging, Grading, and Risk Stratification of Bladder Cancer. European Urology Supplements 2008;7: 618-26.
10. TS
Sağlı
k Bakanlığı Kanser Daire Başkanlığı http://www.ketem.org/istatistik.php erişim
tarihi: 01.02.2015
11. Strom TMW. (2009) Case-control studies. http://ihg2.helmholtz-muenchen.de/cgi-bin/hw/hwa1.pl accessed on:04.03.2015
12. Referans SNF cluster report. http://www.ncbi.nlm.nih.gov/projects/SNP/snp_
ref.cgi?rs=2267437 accessed on: 08.01.2015
13. Referans SNF cluster report II http://www.ncbi.nlm.nih.gov/projects/SNP/snp_
ref.cgi?rs=828907 accessed on: 08.01.2015
14. Bau DT, Tseng HC, Wang CH, Chiu CF, Hua
CH, Wu CN et al. Oral cancer and genetic polymorphism of DNA double strand break gene Ku70 in Taiwan. Oral Oncol
2008;44(11):1047-51.
15. Yang MD, Wang HC, Chang WS, Tsai CW,
Bau DT. Genetic polymorphisms of DNA double strand break gene Ku70 and gastric cancer in Taiwan. BMC Cancer 2011;11:174.
16. He W, Luo S, Huang T, Ren J, Wu X, Shao J,
Zhu Q. The Ku70 -1310C/G promoter
polymorphism is associated with breast cancer susceptibility in Chinese Han population. Mol
Biol Rep 2012;39(1):577-83.
17. Wang HC, Liu CS, Chiu CF, Chiang SY, Wang CH, Wang RF et al. Significant association of DNA repair gene Ku80 genotypes with breast cancer susceptibility in Taiwan. Anticancer Res 2009;29(12):5251-4.
18. Li JQ, Chen J, Liu NN, Yang L, Zeng Y, Wang B et al. Ku80 gene G-1401T promoter polymorphism and risk of gastric cancer. World
J Gastroenterol. 2011;17(16):2131-6.
19. Willems P, De Ruyck K, Van den Broecke R, Makar A, Perletti G, Thierens H et al. Polymorphism in the promoter region of Ku70/XRCC6, associated with breast cancer risk and oestrogen exposure. J Cancer Res Clin
Oncol 2009;135(9):1159-68.

Thank you for copying data from http://www.arastirmax.com