You are here

KONYA'DA YETİŞEN CENTAUREA PTEROCAULA TRUATV 'IN FENOLİK YAPISI VE ANTİOKSİDAN ETKİSİ

DETERMINATION OF PHENOLIC CONTENT AND ANTIOXIDANT EFFECT OF CENTAUREA PTEROCAULA TRUATV. IN KONYA

Journal Name:

Publication Year:

Abstract (2. Language): 
The present study was carried out to determine of Centaurea Pterocaula which was collected in region of Konya Tuz Lake. It was extracted with petroleum ether using a soxhleth apparatus for remove it's oil. Then the defatted plant materials were extracted with 70% methanol.and concentrated. Total phenol concentration was determined by Folin-Ciocalteu method, free radical scavenging activities were determined based on DPPH and determined reducing power based on Oyaizu method. The phenolic compound of C. Pterocaula was elucidated by HPLC analysis. Results were compared with standard antioxidant compound BHA and BHT.
Abstract (Original Language): 
Bu çalışmada Konya Tuz Gölü civarından toplanan Centaurea Pterocaula'nın antioksidan aktivitesinin belirlenmesi amaçlanmıştır. Centaurea Pterocaula 'u yağından uzaklaştırılmak için sokslet apareyi ile petrol eterinde ekstrakte edildi. Sonra materyal %70 lik metanolde tekrar ekstraksiyona tabi tutuldu ve çözücüsünden uzaklaştırıldı. Toplam fenolik madde konsantrasyonu Folin-Ciocalteu metoduna, serbest radikal süpürme etkisi DPPH metoduna göre ve indirgeme gücü ise Oyaizu metoduna göre yapıldı. Bitkinin fenolik yapısı HPLC ile belirlendi. Sonuçlar sentetik antioksidan olan BHT (Butillenmişhidroksitoluen) ve BHA (Butillenmişhidroksianisol) ile kıyaslandı.
35-41

REFERENCES

References: 

ARİF R, KÜPELİ R, ERGUN F, 2004, The Biological Activity of Centaurea L.
Specıes. G.Ü. Fen Bilimleri Dergisi 17(4): 149-164. ÇAKATAY U, KAYALI R, 2006, Serbest Radikal Biyokimyasının Tarihsel Süreçteki
Gelişimi Cerrahpaşa tıp dergisi, 37,162-167. ÇAVDAR C, SİFİL A, ÇAMSARI T, 1997, Reaktif oksijen partikülleri ve
Antioksidan savunma Türk nefroloji ve transplantasyon dergisi, 3-4, 92-95.
SDÜ FEN EDEBİYAT FAKÜLTESİ
FEN DERGİSİ (E-DERGİ). 2008, 3(1), 35-41
41
GAMEZ-MEZA, N, NORİEGA-RODRİGUEZ JA, MEDİNA-JUAREZ LA, ORTEGA-GARCİA, J, CAZAREZ-CASANOVA R, ANGULO-GUERRERO O, 1999, Antioxidant activity in soybean oil of extracts from thompson grape bagasse J.A.O.C.S., 76, 1445.
HUDSON BJF, 1990. Food Antioxidants. Elsevier Applied Science Publishers, New
York. Elsevier,
Ne
w York pp. 253-307. MEİR S, KANNER J, AKİRİ B, and HADAS SP, 1995, Determination and
involvement of aqueous reducing compounds in oxidative defense systems of
various senescing leaves. J. Agric. Food Chem. 43: 1813- 181. MURRAY MT. 1996, Encyclopedia of Nutritional Supplements. California, Prima
Publishing; 1:320-331. NAWAZ H, SHİ J, MİTTAL GS, KAKUDA Y, 2006, Extraction of polyphenols from
grape seeds and concentration by ultrafiltration. Separation and Purification
Technology 48: 176-181 OYAIZU M, 1986. Studies on products of browning reactions: antioxidative activities
of products of browning reaction prepared from glucosamine. Japanese Journal
of Nutrition, 44, 307-315.
POURMORAD F, HOSSEINIMEHR SJ, SHAHABIMAJD N, 2006, Antioxidant
activity, phenol and flavonoid contents of some selected Iranian medicinal
plantsAfrican Journal of Biotechnology., 5 (11), 1142-1145. PRATT DE, HUDSON, BJF, 1990. Natural Antioxidant not Exploited Commercially,
in Food Antioxidants, 5, 171. PROESTOS C, SERELİ D, KOMAİTİS, 2006, Determination of phenolic compounds
in aromatic plants by RP-HPLC and GC-MS, Food Chemistry 95:44-52. RIMMER DL, 2006, Free radicals, antioxidants, and soil organic matter recalcitrance
Journal of Soil Science 57(2): 91-94. SHAHİDİ, F. 2000, Antioxidants in Food and Food Antioxidants. Nahrung, 44,158-163.
SOONG YY, BARLOW PJ, 2004. Antioxidant activity and phenolic content of
selected fruit seeds. Food Chemistry 88, 411-417 TUNALIER Z, ÖZTÜRK N, KOŞAR M, BAŞER KHC, DUMAN H, KIRIMER N,
2002, Bazı sideritis türlerinin antioksidan etki ve fenolik bileşikler yönünden
incelenmesi, Bitkisel İlaç Hammaddeleri Toplantısı. VAYA J AND AVİRAM M. 2001, Nutritional Antioxidants: Mechanisms of Action,
Analyses of Activities and Medical Applications Curr. Med. Chem. - Imm., Endoc. & Metab. Agents, 1, 99-117.
WANG CK, LEE, WH, 1996, Separation, Characteristics, and Biological Activities of
Phenolics in Areca Fruit, J.Agric.Food.Chem., 44: 2014-2019 YANİSHLİEVA NV, POKOMY J, GORDON, M. 2001, Inhibiting Oxidation in
Antioxidants in Food: Practical Applications., CRC press LLC and Woodhead Publishing Ltd, 288s, New York, USA.

Thank you for copying data from http://www.arastirmax.com