You are here

TUZ STRESİ (NaCl) ALTINDA ÇİMLENDİRİLEN ARPA TOHUMLARININ MİTOTİK İNDEKS VE KROMOZOM ANORMALLİKLERİ ÜZERİNE BAZI BİTKİ BÜYÜME DÜZENLEYİCİSİ KOMBİNASYONLARININ ETKİLERİ

EFFECTS OF COMBINATIONS OF SOME PLANT GROWTH REGULATORS ON MITOTIC INDEX AND CHROMOSOMAL ABERRATIONS OF BARLEY SEEDS GERMINATED UNDER SALINE (NaCl) CONDITIONS

Journal Name:

Publication Year:

Abstract (2. Language): 
In this work, the effects of double, triple and quadruple combinations of gibberellic acid (GA3), kinetin (Kin), benzyladenine (BA), 24-epibrassinolide (EBR), ethylene (E) polyamines (cadaverine, putrescine, spermidine, spemine) on the mitotic index and chromosomal abberations of barley seeds (Hordeum vulgare L. var. Bülbül 89) germinated under saline conditions were studied. In paralel to the rises in salt concentrations rise, mitotic activity significantly decreased and percantage of chromosomal abberation increased. Most of the plant growth regulator combinations studied showed positive effect on mitotic activity in high salt concentrations. Besides, most of this growth regulators were succesful in alleviating of the negative effect of percantage of chromosomal abberation in all the salt levels. Consequently, it was determined that plant growth regulator combinations mentioned affected in different degrees on mitotic index and chromosomal abberations of barley seeds and this difference was statistically important.
Abstract (Original Language): 
Bu çalışmada tuz stresi altında çimlendirilen arpa tohumlarının (Hordeum vulgare L. var. Bülbül 89) mitotik indeks ve kromozom anormallikleri üzerine gibberellik asit (GA3), kinetin (Kin), benziladenin (BA), 24-epibrassinolid (EBR), etilen (E) ve poliamilerin (kadaverin-Kad, putressin-Put, spermidin-Spd, spemin-Spm) ikili, üçlü ve dörtlü kombinasyonlarının etkileri araştırılmıştır. Tuz konsantrasyonunun artışına paralel olarak mitotik aktivite önemli ölçüde azalmış ve kromozom anormallik yüzdesi artmıştır. Çalışılan büyüme düzenleyicisi kombinasyonlarının büyük bir çoğunluğu yüksek tuz konsantrasyonlarında mitotik aktivitesi üzerinde olumlu bir etki göstermiştir. Ayrıca bu büyüme düzenleyicilerinin büyük bir çoğunluğu tüm tuz seviyelerinde kromozom anormallik yüzdesi üzerindeki olumsuz etkiyi önemli ölçüde hafifletmiştir. Sonuç olarak, söz konusu bitki büyüme düzenleyicisi kombinasyonlarının arpa tohumlarının mitotik indeks ve kromozom anormallikleri üzerinde farklı derecelerde etkili oldukları ve bu farkın istatistiksel açıdan önemli olduğu tespit edilmiştir
162-173

REFERENCES

References: 

AL-KARAKI GN,
2001
. Germination, sodium and potassium concentration of barley seeds as
influenced by salinity. Journal of Plant Nutrition, 24, 511-522. ANURADHA S, RAO SSR, 2001. Effect of brassinosteroids on salinity stress induced
inhibition of seed germination and seedling growth of rice (Oryza sativa L.). Plant
Growth Regulation, 33 (2), 151-153. ASHRAF MY, SARWAR G, ASHRAF M, AFAF R, SATTAR A, 2002. Salinity induced
changes in a-amylase activity during germination and early cotton seedling growth.
BiologiaPlantarum, 45 (4), 589-591. BARLOW PW, 1976. Towards an understanding of the behavior of root meristems. Journal
of Theoretical Biology, 57, 433-451. BESNARD-WIBANT C, NOIN M, ZEEVAART JAD, 1983. Mitotic activities and levels of
nuclear DNA in the apical meristem of Silene armeria (strain SI.2) following
application of gibberellin A3. Plant and Cell Physiology, 24, 1269-1279.
170
S. TABUR, K. DEMİR
BOZCUK S, 1978. Domates (Lycopersicum esculentum Mill.), arpa (Hordeum vulgare L.) ve pamuk (Gossypium hirsitum L.) bitkilerinin büyüme ve gelişmesinde tuz-kinetin etkileşimi üzerinde araştırmalar, Doçentlik tezi, Hacettepe Üniversitesi, Fen Fakültesi. BRAUN JW, KHAN AA, 1976. Alleviation of salinity and high temperature stress by plant growth regulators permeated into lettuce seeds via acetone. Journal of the American Society for Horticultural Science, 101, 716-721. CLOUSE SD, ZUREK D, 1991. Molecular analysis of brassinolide action in plant growth and
development. Acs-Symposium Series American Chemical Society, 474, 122-140. COSTA G, BAGNI N, 1983. Effects of polyamines on fruit-set of apple. Hortscience, 18 (1),
59-61.
DASH M, PANDA, SK, 2001. Salt stress induced changes in growth and enzyme activities in
germinating Phaseolus muingo seeds. Biologia Plantarum, 44, 587-589. DE AGAZIO M, FEDERICO R, ANGELINI R, DE CESARE F, GREGO S, 1992. Spermidine pretreatment or root tip removal in maize seedlings: effect on K+ uptake and tissues modifications. Journal of Plant Physiology, 140, 741-746. ELÇİ Ş, 1982. Sitogenetikte Gözlemler ve Araştırma Yöntemleri. Fırat Üniversitesi Fen
Edebiyat Fakültesi Yayınları, 3, 37-85. Elazığ. EL-MASHAD AA, KAMEL EA, 2001. Amelioration of NaCl stress in Pisum sativum. Indian
Journal
Experimental Biology, 39(5), 469-475. FOX DP, 1969. Some characteristic of the cold hydrolysis technique for staining plant tissues
by the feulgen reaction. Journal of Histochemistry and Cytochemistry, 17, 266. GATTA L, MARHITELLI C, FEDERICO R, 1992. Effect of polyamines and their oxydative
product on maize and lentil root growth. Annals of Botany, 50, 43-48. GILL KS, SINGH OS, 1985. Effect of salinity on carbohydrate metabolism during paddy (Oryza sativa) seed germination under salt stress condition. Journal of Experimental
Biology, 23, 384-386.
HOWELL WM, KELLER III GE, KIRKPATRICK JD, JENKINS RL, HUNSINGER RN, MCLAUGHLIN EW, 2007. Effects of the plant steroidal hormone, 24-
epibrassinolide, on the mitotic index and growth of onion (Allium cepa) root tips. Genetics Molecular Research, 6 (1), 50-58. HU Y, BAO F, Lİ J, 2000. Promotive effect of brassinosteroids on cell division involves a
distinct CycD3-induction pathway in Arabidopsis. Plant Journal, 24, 693-701. HUANG CX, VAN STEVENINCK RFM, 1988. Effect of moderate salinity on patterns of potassium, sodium and chloride accumulation in cells near the root tip of barley: role of differentiating metaxylem vessels. Physiologia Plantarum, 73, 525-533. HUANG CX, VAN STEVENINCK RFM, 1990. Salinity induced structural changes in
meristematic cells of barley roots. New Phytologist, 115, 17-22. İSMAİLOĞLU I, ÜNAL M, PALAVAN-ÜNSAL N, 2004. Effects of spermidine, spermine and cyclohexylamine on mitotic activity of 2X, 4X and 6X wheats. Journal of Cell and Molecular Biology, 3, 83-88. KABAR K, 1987. Alleviation of salinity stress by plant growth regulators on seed
germination, Journal of Plant Physiology, 128, 179-183. KABAR K, 1989. Interactions among salt (NaCI), kinetin and gibberellic acid in the
germination of Lettuce seeds. Doğa Turkish Journal of Botany, 13, 296-300. KABAR K, BALTEPE Ş, 1987. Alleviation of Salinity Stress on Germination of Barley
Seeds by Plant Growth Regulators. Doğa Turkish Journal of Biology, 11, 108-117. KATSUHARA M, KAWASAKI T, 1996. Salt stress induced nuclear and DNA degradation
in meristematic cells of barley roots. Plant and Cell Physiology, 37, 169-173. KHAN AA, 1971. Cytokinin: Permissive role in seed germination. Science, 171, 853-859.
171
SDÜ FEN EDEBİYAT FAKÜLTESİ
FEN DERGİSİ (E-DERGİ). 2008, 3(2) 162-173
KHAN AA, 1978. Incorporation of bioactive chemicals into seeds to alleviate. environmental stres. Acta Horticulturae, 83, 225-234.
KHAN MA, 1991. Studies on germination of Cressa cretica. Pakistan Journal of Weed Science Research, 4, 89-98.
KHAN MA, WEBER DJ, 1986. Factors influencing seed germination in Salicornia pacifica var. Utahensis, American Journal of Botany, 73, 1163-1167.
LIU PBW, LOY, JB, 1976. Action of gibberellic acid on cell proliferation in the subapical shoot meristem of watermelon seedlings. American Journal of Botany, 63 (5), 700¬704.
LUTSENKO EK, MARUSHKO EA, KONONENKO NV, LEONOVA TG, 2005. Effects of
fusicoccin on the early stages of sorghum growth at high NaCl concentrations.
Russian Journal of Plant Physiology, 52, 332-337. MIRZA JI, BAGNI N, 1991. Effects of exogenous polyamines and difluoromethylornithine
on seed germination and root growth of Arabidopsis thaliana. Plant Growth
Regulation, 10, 163-168. MIYAZAWA Y, NAKAJIMA N, ABE T, SAKAI A, 2003. Activation of cell proliferation by
brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell
multiplication, cell-cycle-related gene expression, and organellar DNA contents.
Journal
of Experimental Botany, 54, 2669-2678. MUTLU F, BOZCUK S, 2000. Tuzlu koşullarda ayçiçeği tohumların çimlenmesi ve erken
büyüme üzerine dışsal spermin'in etkileri.
Turkish
Journal
of Biology, 24, 635-643. NIEMAN RH, 1965. Expansion of bean leaves and its suppression by salinity. Plant
Physiology, 40, 156-161. OH MH, CLOUSE SD, 1998. Brassinosteroid affects the rate of cell division in isolated leaf
protoplasts of Petunia hybrida. Plant Cell Reports, 17, 921-924. PALAVAN-ÜNSAL N, 1987. Polyamine metabolism in the root of Phaseolus vulgaris
interaction of the inhibitors of polyamine biosynthesis with putrescine in growth and
polyamine biosynthesis. Plant Cell Physiology, 28, 565-572. PALAVAN-ÜNSAL N, ZEHİR Z, SAĞLAM S, 1990. Inhibition of alpha-amylase release
from germinating wheat embryos by abcisic acid cyclohexylammonium, polyamines
and ethylene. In: FLORES HE. ARTECA RN. SHANNON JC. (Eds.) Biochemistry,
Physiology and Interactions. American Society of Plant Physiologist, 353-357. PALMER MV, GUNNING BES, 1984. Cytokinin-induced mitosis in cultured explants of
Helianthus tuberosus L. tuber tissue. Australian Journal of Plant Physiology, 11, 1-6. PRAKASH L, PRATHAPASENAN G, 1988. Effect of NaCl salinity and putrescine on shoot
growth, tissue ion concentration and yield of rice. Journal of Agronomy and Crop
Science, 160, 325-334.
PRAKASH L, PRATHAPASENAN G, 1990. Interactive effect of. NaCl salinity and
gibberellic acid and gibberellin like substances and yield of rice (Oryza sativa L. var.
G.R.3). Proceedings of the Indian Academy Science, 100, 173-181. RAO VS, SANKHLA N, KHAN AA, 1975. Additive ergistic effects of kinetin and ethrel on
germination, thermodormancy and polyribosome formation in lettuce seeds. Plant
Physiologia, 56, 263-266. ROST S, FRANK C, BECK E, 1996. The chloroplast envelope is permeable for maltose but
not for maltodextrins. Biochimica et Biophysica Acta, 1291, 221-227. SALA C, SALA F, 1985. Effect of brassinosteroid on cell division and enlargement in culture
carrot (Daucus carota L.) cells. Plant Cell Reports, 4, 144-147. SALISBURY FB, ROSS CW, 1992. Plant Physiology. Belmont, CA: Wadsworth, 682.
172
S. TABUR, K. DEMİR
SCHMIDHALTER U, OERTLI JJ, 1991. Germination and seedling growth of carrots under
salinity and moisture stres. Plant and Soil, 132, 243-251. SHEORAN IS, 1980. Changes in amylase during germination and early seedling growth of
mungbean (Vigna radiata L. Eilczek) under different salts. Indian Journal of Plant
Physiology, 23, 169-173. SMITH TA, BEST GR, 1977. Polyamines in barley seedlings. Phytochemistry, 16, 814-843.
TAJBAKHSH M, ZHOU MX, CHEN ZH, MENDHAM NJ, 2006. Physiological and
cytological response of salt-tolerant and non-tolerant barley to salinity during germination and early growth. Australian Journal of Experimental Agriculture, 46 (4),
555-562.
TAL M, 1977. Physiology of polyploid plants: DNA, RNA, protein and abscisic acid in
autotetraploid and diploid tomato under low and high salinity. Botanical Gazette, 138. TOMASZEWSKA-SOWA M, DROZDOWSKA L, SZOTA M, 2002. Effect of cytokinins on
in vitro morphogenesis and ploidy of pepper Capsicum annuum L. Electronic Journal
of Polish Agricultural Universities, Agronomy, 5, Issue 1. UNGAR IA, BINET P, 1975. Factors influencing seed dormancy in Spergularia media (L.)
C. Presl. Aquatic Botany, 1, 45-55.
ÜNAL
M.
, PALAVAN-ÜNSAL N, TÜFEKÇİ MA, 2002. Role of putrescine and its
biosynthetic inhibitor on seed germination root elongation and mitosis in Hordeum vulgare L. Bulletin Pure and Applied Science Section B-Botany, 21, 33-38.
VAN-DERZANDEN AM, 1999. Oregon State University Extension Service, Master Gardener Handbook, Chapter 1, Botany Basics, İnternet Sitesi: http://extension.oregonstate.edu/mg/botany/hormones.html Erişim Tarihi:
04.05.2006.
YÜREKLİ
F
, TÜRKAN I, PORGALI ZB, TOPÇUOĞLU F, 2001. Indoleacetic acid, giberellic acid, zeatin and abscisic acid levels in NaCl-treated tomato species differing in salt tolerance. Israel Journal of Plant Science, 49 (4), 269-277.
YÜREKLİ
F
, PORGALI ZB, TÜRKAN I, 2004. Variations in abscisic acid, indole-3acetic
acid, gibberellic acid and zeatin concentrations in two bean species subjected to salt stress. Acta Biologia Cracovensia Series Botanica, 46, 201-212. ZHU JK, 2001. Over expession of a delta-pyroline-5-carboxylate synthetase gene and analysis of tolerance to water and salt stres in transgenic rice. Trends in Plant Science,
6, 66-72.

Thank you for copying data from http://www.arastirmax.com