You are here

Home » Content

Farklı Dozlarda Ardıç Yağının Sıçan Karaciğerinde Antioksidan Enzimler Üzerine Etkisi

Journal Name:

  • Süleyman Demirel Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: Oil of Juniperus Communis Lynn (JCL) which is extracted from JCL leaves is a natural product. It has been commonly used for treating hypercholesterolemia, diabetes as herbal treatment. As these diseases become more frequent and progress over aging the usage of JCL oil prolongs and the doses used increase. In the present study we investigated the effects of JCL oil on oxidative and anti–oxidative systems in liver tissue of hypercholesterolemic rats which were administered JCL oil to lower their cholesterol levels. Material–Method: Thirty–five, adult male Wistar albino rats were included and divided into 5 groups. The control group (GI) was fed with normal pellet chow, the cholesterol (GII) group was fed with pellet chow containing 2% of cholesterol, the 3rd, 4th and 5th groups were fed with 50, 100, 200mg/kg JCL oil, respectively in addition to the 2% cholesterol–containing pellet chow. At the end of 30 days the experiment was ended and liver tissues were taken. Oxidative (MDA) product and anti– oxidative (KAT, SOD, GSH–Px) enzymes were detected. Results: MDA levels of the groups that were fed with cholesterol were significantly increased compared with control group (p<0.01). The dose of 200mg/kg JCL (GV) did not lower even increased the MDA levels compared to GII, CAT and SOD activities in GV were significantly decreased compared to GI (p<0.01). Discussion: JCL oil provides antioxidant effect with the dose 50mg/kg but this effect did not prolong with higher doses in fact with the dose 200 mg/kg oxidative stress was increased. In conclusion it can be said that JCL oil may lead to different effects in a tissue–specific and dose– dependent manner.
Bookmark/Search this post with
Abstract (Original Language): 
Amaç: Ardıç yağı, Juniperus Communis Lynn (JCL)’den elde edilen doğal bir üründür. Halk arasında hiperkolesterolemi, diabet gibi hastalıklarda bitkisel tedavi alanında yaygın olarak kullanılmaktadır. Bu tür hastalıkların sıklığının artışı ve hastalık sürecinin uzayışı ileri yaşlarda aşikardır. Dolayısıyla bu yaşlarda ardıç yağı kullanımı ve dozu artmaktadır. Biz çalışmamızda kolesterol düşürücü amaçla 30 gün kullanılan ardıç yağının karaciğer oksidatif ve antioksidatif sistemine etkilerini araştırmayı amaçladık. Gereç–Yöntem: 35 erişkin, erkek, Wistar albino sıçanlar, kontrol (GI), kolesterol (GII), 50 mg/kg JCL + kolesterol (GIII), 100 mg/kg JCL + kolesterol (GIV) ve 200 mg/kg JCL + kolesterol verilen grup (GIV) olmak üzere 5 gruba ayrılmıştır. Otuz günlük uygulama sonunda sıçanlar anestezi altında sakrifiye edilerek karaciğer dokuları alınmıştır. Karaciğer dokusunda malondialdehid (MDA), süperoksit dismutaz (SOD), katalaz (KAT) ve glutatyon peroksidaz (GSH–Px) düzeyleri saptanmıştır. Sonuçlar: Kolesterol alan tüm gruplarda MDA düzeyi anlamlı düzeyde yükselmiştir. JCL verilmesi özellikle 200 mg/kg ile MDA düzeyini düşürememiş hatta GII’ye göre dahi anlamlı düzeyde yükseltmiştir. SOD ve KAT aktiviteleri de 200mg/kg JCL yağı alan grupta anlamlı düzeyde azalmıştır (P< 0.01). Tartışma: JCL yağı karaciğer dokusunda 50 mg/kg doz ile antioksidan, 200 mg/kg doz ile oksidatif stresi arttırıcı özellik göstermektedir. Vücutta JCL kullanılan doza bağımlı olarak dokuya özgü farklı sonuçlar yaratabilmektedir.
FULL TEXT (PDF): 
PDF icon arastirmax-farkli-dozlarda-ardic-yaginin-sican-karacigerinde-antioksidan-enzimler-uzerine-etkisi.pdf
  • 2
77-81
  • Turkish

REFERENCES

References: 

1. Innocenti M, Michelozzi M, Giaccherini C,
Ieri F, Vincieri FF, Mulinacci N. Flavonoids
and biflavonoids in Tuscan berries of Juniperus
communis L.: detection and quantitation by
HPLC–DAD–ESI–MS. J. Agric. Food Chem.
2007; 55 (16): 6596– 6602.
2. ESCOP. Juniperi fructus: Monographs on the
Medici–nal Uses of Plant Drugs. Exeter, U.K.:
European Scien–tific Cooperative on Phytotherapy
1997.
3. Petlevski R, Hadzija M, Slijepcevic M, Juretic
D. Effect of ‘antidiabetis’ herbal preparation on
serum glucose and fructosamine in NOD mice. J.
Ethnopharm., 2001; 75: 181–185.
4. Sa´nchez de Medina, F, Ga´mez MJ, Jime´nez
I, Jime´nez J, Osuna JI and Zarzuelo A.
Hypoglycemic activity of juniper “berries”. Planta
Med., 1994; 60: 197–200.
5. Ritch–Krc EM, Thomas S, Turner NJ, Towers
GHN. Carriere herbal medicine: traditionally and
contemporarily use. J. Ethnopharm. 1996; 52:
85–94.
6. Newall CA, Anderson LA, Phillipson J.D. Herbal
Medicines. A Guide for Health–Care Professionals.
London: The Pharmaceutical Press. 1996.
7. Agrawal OP, Bharadwaj S, Mathur R. Antifertility
effects of fruit of Juniperus communis. Planta
Med., 1980; 98–101.
8. Gardner DR, Panter KE, James LF, Stegelmeier
BL. Abortifacient effects of lodgepole pine
(Pinus contorta) and common juniper (Juniperus
communis) on cattle. Vet. Hum. Toxicol. 1998, 40:
260–263.
9. Baytop T. Therapy with Medicinal Plants in
Turkey (Past and Present); Nobel Tıp Kitapevleri;
152–153, 1999.
10. Asımgil A. Şifalı Bitkiler. Timaş Yayınevi, 1997;
38–39.
11. Pappas SR. Institüt für Marktokologie. Nr. 22/183.
Vagosca, Bosnia – Herzegovina 2003.
12. Newall CA, Anderson LA, Phillipson J.D. Herbal
Medicines. A Guide for Health–Care Professionals.
London: The Pharmaceutical Press. 1996.
13. Butani L, Afshinnik A, Johnson J, et al. Amelioration
of tacrolimus–induced nephrotoxicity in rats using
juniper oil. Transplantation 2003; 76(2): 306–311.
14. Syrov VN, Abzalova MK, Khushbaktova
ZA, Sultanov MB. Hypolipidemic and
antiatherosclerotic properties of terpenoid
coumarins. Institute of the Chemistry of Plant
Substances, Academy of Sciences of the Uzbek
SSR, Tashkent Received 1984.
15. Akdogan M, Koyu A, Ciris M, Yildiz K. Anti–
hypercholesterolemic activity of Juniperus
communis Lynn Oil in rats: A biochemical and
histopathological investigation. Biomedical
Research 2012; 23 (3), 321–328.
16. Burçak G, Andican G. Oksidatif DNA Hasarı ve
yaşlanma. (Oxidative DNA damage and aging)
Cerrahpaşa J Med 2004; 35: 159–169.
17. Smith C, Marks A, Lieberman M. Marks’ Basic
Medical Biochemistry. A Clinical Approach.
Second Edition. Section 4. Chapter 24. Oxygen
Toxicity and Free Radical Injury. 439–457.
18. 18. Halliwell, B. and Gutteridge, J.M.C. 1984.
Oxygen toxicity, oxygen radicals, transition
19. metals and disease. Biochem. J., 219: 1–4.
20. Ho CT, Osawa T, Huang MT, Rosen, T. Food
phytochemicals for cancer prevention II. ACS
Symphosium. Series 547. American Chemical
Society: Washington, DC. 1994.
21. Burits M, Asres K, Bucar F. The antioxidant activity
of the essential oils of Artemisia afra, Artemisia
abyssinica and Juniperus procera. Phytother Res
2001; 15(2):103–8.
22. Afanasev IB, Dorozhko AL, Brodoski AV, et al.
Chelating and free radical scavenging mechanisms
of inhibitory action of rutin and quercetin in lipid
peroxidation. Biochem Pharmacol 1989; 38:
1763–1769.
23. Elmastas M, Gulcin I, Beydemir S, Kufreviogulu
OI, et al. A Study on the In Vitro Antioxidant
Activity of Juniper (Juniperus communis L.) Fruit
Extracts. Ana–lytical Letters 2006; 39: 47–65.
24. Kumar P, Chandra H, Singh A, Bhatt RP.
Antioxidant and anticandidal activity of
Juniperus communis L. International Journal of
Environmental Rehabilitation and Conservation
2010; 1(1):10–16.
25.Miceli N, Trovato A, Dugo P, Cacciola F, Donato
P, Marino A, Bellinghieri V, La Barbera TM,
Güvenç A, Taviano MF. Comparative analysis of
flavonoid profile, antioxidant and antimicrobial
activity of the berries of Juniperus communis L.
var. communis and Juniperus communis L. var.
saxatilis Pall. from Turkey, J Agric Food Chem.
2009; 57 (15): 6570 – 6577.
26. Lowry OH, Rosebrough NJ, Farr AL, Randall
RJ. Protein measurement with the folinphenol
reagents. J Biol Chem. 1951; 193: 265–275.
27. Drapper HH, Hadley M. Malondialdehyde
determination as index of lipid peroxidation.
Methods Enzymol 1990; 186: 421–431.
28. Aebi H. Catalase in vitro. Methods Enzymol.
1984;105:121–26.
29. Paglia DE, Valentine WN. Studies on the
quantitative and qualitative characterization of
erythrocyte glutathione peroxidase. J Lab Clin
Med. 1967; 70: 158–69.
30.Woolliams JA, Wiener G, Anderson PH, McMurray
CH. Variation in the activities of glutathione
peroxidase and superoxide dismutase and in the
concentration of copper in the blood various breed
crosses of sheep. Res Vet Sci. 1983; 34: 69–77.
31. Balkana J, Doğru AS, Aykaç TG, Uysal M. The
effect of a highcholesterol diet on lipids and
oxidativestress in plasma, liver and aorta of rabbits
and rats. Nutrition Research, 2004; 24 (3): 229–
234.
32.Mahfouz MM, Kummerow FA. Cholesterol–rich
diets have different effects on lipid peroxidation,
cholesterol oxides, and antioxidant enzymes in rats
and rabbits. J Nutr Biochem, 2000; 11: 293–302.
33. Emami SA, Javadi B, Hassanzadeh MK.
Antioxidant Activity of the Essential Oils of
Different Parts of Juniperus communis. subsp.
hemisphaerica. and Juniperus oblonga. 2007; 45
(10): 769–776.
34.Murray RK, Granner DK, Mayes PA, Rodwell
VW. Harper’in Biyokimyası. 24. Baskı. Bölüm 4,
61. Ünite, 799–807.

Thank you for copying data from http://www.arastirmax.com