You are here

Home » Content

Chlorpyriphos-ethyl'in rat plazmasında in vivo lipoperoksidatif etkisi ile melatonin ve vitamin C +vitamin E'nin koruyucu etkilerinin araştırılması

Investigation of the in vivo lipoperoxidative effect of chlorpyriphos-ethyl in plasma of rats and ameliorating effects of melatonin and the combination of vitamin C + vitamin E on this effect

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2003

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The lipoperoxidative effect of chlorpyriphos-ethyl (CE) and ameliorating effect of melatonin and the combina¬tion of vitamin C + vitamin E in plasma of CE exposed rats were investigated. The experimental groups were as follows: Control, CE treated group (CE), vitamin E + vitamin C treated group (Vit), melatonin treated group (Mel), vitamin E + vitamin C + CE treated group (Vit+CE), and melatonin + CE treated group (Mel+CE). While vitamins and melatonin were administered 6 consecutive days, CE was administered only 5th and 6th days to the appropriate groups. The blood samples were taken by appropriate techniques for biochemical examinations. The levels of thiobarbituric acid reactive substances (TBARS), and antioxidant potential (AOP) were determined in plasma.TBARS was found to be increased in CE Group compared to Control Group , whereas it was found to be decreased in Vit+CE Group and Mel+CE Group compared to CE Group (p<0.05). Conversely, AOP was found to be decreased in CE Group compared to Control Group, whereas it was found to be increased in Vit+CE Group and Mel+CE Group compared to CE Group (p<0.05). These results suggested that treating CE increases lipid peroxidation and decreases AOP by increas¬ing oxidative stress in plasma of rats, and melatonin and the combination of vitamin C + vitamin E can reduce this lipoperoxidative effect of CE.
Bookmark/Search this post with
Abstract (Original Language): 
Chlorpyriphos-ethyl'e (CE) maruz kalan ratların plazmalarında melatonin ile vitamin C ve vitamin E kombinasyonunun koruyucu etkileri araştırıldı. Deney grupları şu şekilde organize edildi: Kontrol grubu (C), CE verilen grup (CE), vitamin E + vitamin C verilen grup (Vit), melatonin verilen grup (Mel), vitamin E + vitamin E + CE verilen grup (Vit+CE) ve melatonin + CE verilen grup (Mel+CE). İlgili gruplara altı gün vitaminler ve melatonin verilirken beşinci ve altıncı gün CE verildi. Uygun teknikle biyokimyasal çalışmalar için kan örnekleri alındı. Plazmada Tiobarbitürik Asitle Reaksiyon Veren Substratlar (TBARS) ve Antioksidan Potansiyel (AOP) düzeyleri ölçüldü. TBARS CE grubunda kontrol grubuna göre yüksek iken, CE grubu ile karşılaştırıldığında Mel+CE ve Vit+CE gruplarında anlamlı olarak düşük bulundu. AOP ise TBARS'ın tersine kontrol grubu ile karşılaştırıldığında CE grubunda düşük bulunurken, CE grubu ile karşılaştırıldığında Mel+CE ve Vit+CE gruplarında anlamlı olarak yüksek bulundu (p<0.05). Sonuçta, CE'nin rat plazmasında oksidatif stresi artırmak suretiyle lipid peroksidasyonunu artırdığı ve AOP'yi düşürdüğü, melatoninin ile vita¬min C + vitamin E kombinasyonunun CE'nin bu lipoperoksidatif etkisini düşürebileceği kanaatine varıldı.
FULL TEXT (PDF): 
PDF icon arastirmax-chlorpyriphos-ethylin-rat-plazmasinda-vivo-lipoperoksidatif-etkisi-ile-melatonin-vitamin-c-vitamin-enin-koruyucu-etkilerinin-arastirilmasi.pdf
  • 2
24-28
  • Turkish

REFERENCES

References: 

1. Hunter DL, Lassiter TL, Padilla S. Gestational exposure to chlorpyrifos: comparative distribution of trichloropyridi-nol in the fetus and dam. Toxicol Appl Pharmacol 1999; 158(1): 16-23.
2. Li WF, Furlong CE, Costa LG. Paraoxonase protects against chlorpyrifos toxicity in mice. Toxicol Lett 1995;
76(3): 219-26.
3. Chanda SM, Mortensen SR,Moser VC, Padilla S. Tissue-specific effects of chlorpyrifos on carboxylesterase and cholinesterase activity in adult rats : an in vitro and in vivo comparison. Fundam Appl Toxicol 1997; 38(2): 148-57.
4. Bigbee JW, Sharma KV, Gupta JJ, Dupree JL. Morfogenik role for acetylcholinesterase in axonal out¬growth during neural development. Environ Health
Perspect 1999; 1: 81-7.
5. Agrawal D, Sultana P, Gupta G.S.D. Oxidative damage and changes in the glutathione redox system in erythro-cytes from rats treated with hexachlorocyclohexane Food
Chem Toxicol 1991; 29(7): 459-62.
6. Stephen B, Kyle L, Yong X, Cynthia A, Donald E, Earl F, James E. Role of oxidative stress in the mechanism of
Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2003:10-( 2 ) / 2 4 - 2 8
28
Kılınç,
Chlorpyriphos-ethy
l
dieldrin's hepatotoxicity. Ann Clin Lab Sci 1997; 27(3): 196-208.
7. Bagchi D, Bagchi M, Hassoun EA, Stohs SJ. In vitro and in vivo generation of reactive oxygen species, DNA dam¬age and lactate dehydrogenase leakage by selected pesti¬cides. Toxicology 1995; 104(1-3): 129-40.
8. Cheeseman KH, Slater TF. An introduction to free radi¬cal biochemistry. Br Med Bull 1993; 49(3): 479-80.
9. Gultekin F, Ozturk M, Akdogan M. (2000) The effect of
organophosfhate insecticide chlorpyrifos-ethyl on lipid peroxidation and antioxidant enzymes (in vitro). Archives of Toxicology, 74: 533-538.
10. Draper HH, Hadley M. Malondialdehyde determina¬tion as index of lipid peroxidation. Methods Enzymol 1990; 186:421-31.
11. Durak I, Karabacak HI, Büyükkocak S, Çimen MYB, Kaçmaz M, Ömeroğlu E, Öztürk HS. Impaired antioxidant defence system in the kidney tissues from rabbits treated with cyclosporine. Nephron 1998; 78:207-11.
12. Klaunig JE, Xu Y, Isenberg JS, Bachowski S, Kolaja KL, Jiang J. The role of oxidative stress in chemical carcinogenesis. Environ Health Perspect 1998; 1:289 -95.
13. Steevens JA, Benson WH. Toxicological interactions of chlorpyrifos and methyl mercury in the amphipod, Hyalella azteca. Toxicol Sci 1999; 52(2): 168-77.
14. Lodowici M, Aiolli S, Monserrat C, Dolara P, Medica A, Symlicio P. Effect of a mixture of 15 commonly used pesticides on DNA levels of 8-hydroxy-2-deoxyguanosine and xenobiotic metabolizing enzymes in rat liver. J Environ Pathol Toxicol Oncol 1994; 13(3): 163-8.
15. Jayati Gupta , Chhabi Datta. Effect of malathion on antioxidant defence system in human fetus-An in vitro
study. Ind J Exp Biol 1992; 352-4.
16. Datta C, Gupta J, Sarkar A, Sengupta D. Effects of organophosphorus insecticide phosphomidon on antioxi-dant defence components of human erythrocyte and plas¬ma. Ind J Exp Biol 1992; 30: 65-7.
17. Dwivedi PD, Mukul D, Khanna SK. Role of
cytochrome p-450 in quinalphos toxicity : Effect on hepat¬ic and brain antioksidant enzymes in rats. Food Chem
Toxicol 1998; 36: 437-44.
18. Prasanta KM, Anand K. Dimethoate inhibits extrathy-roidal 5' - monodeiodination of thyroxine to 3,3', 5- tri-iodothyronine in mice : the possible involvement of the lipid peroxidative process. Toxicol Lett 1997; 91: 1-6.
19. Lunec J, Blake D, Oxygen Free Radicals : Their rele¬vance to disease processes . In: Cohen RD, Lewis B, Albert KGMM. The Metabolic and Moleculer Basis of Acquired
Disease. Balliere Tindall, London, 1990; 189-212.
20. Jialal I, Fuller CJ. Oxidized LDL and antioxidants . Clin Cardiol 1993; 16: 1-9.
21. Mayes PA. Structure and Function of the Water-soluble Vitamins. In : Murray RK, Granner DK, Mayes PA -Rodwell VW. Harper's Biochemistry. 23. ed. Lange med¬ical publication, London, 1993; 573-87.
22. Gey KF. Prospects for the prevention of free radical dis¬ease, regarding cancer and cardiovascular disease . Br Med
Bull 1993; 49(3): 679-99.
23. Biri H, Ozturk HS, Buyukkocak S, Kacmaz M, Cimen MY, Unal D, Birey M, Bozkirli I, Durak I. Antioxidant
defense potential of rabbit renal tissues after ESWL: pro¬tective effects of antioxidant vitamins. Nephron 1998; 79(2): 181-5.
24. Campisi A, Di Giacomo C, Russo A, Sorrenti V, Vanella G, Acquaviva R, Li Volti G, Vanella A. Antioxidant systems in rat lens as a function of age: effect of chronic administration of vitamin E and ascorbate. Aging (Milano) 1999; 11(1): 39-43.
25. Ozguner F, Kerman M, Delibas N, Gultekin F. The rela¬tionship of age related decrease in melatonin with oxidative damage and mental status. Biomed Res 2000; 11(1): 61-5.
26. Melchiorri D, Reiter RJ, Attia AM, Hara M, Burgos A, Nistico G. Potent protective effect of melatonin on in vivo paraquat-induced oxidative damage in rats. Life Sci 1994;
56: 83-9.
27. Severynek E, Melchiorri D, Reiter RJ, Ortiz GG,Lewinski A. Lipopolysaccharide- induced hepatoxicity is inhibited by the antioxidant melatonin. Eur J Pharmacol
1995; 293: 327-34.
28. Daniels WMV, Reiter RJ, Melchiorri D, Severynek E,
Pablos MI, Ortiz GG. Melatonin counteracts lipid peroxi-dation induced by carbon tetrachloride but does not restore glucose-6-phosphatase activity. J Pineal Res 1995; 19: 1-6.
29. Melchiorri D, Reiter RJ, Sewerynek E, Chen LD, Nistico G. Melatonin reduces kainate-induced lipid perox-idation in homogenates of different brain regions. FASEB J 1995; 9: 1205-10.

Thank you for copying data from http://www.arastirmax.com