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2,4-Diklorofenoksiasetik asit'i n ikinci çapraz yavruların böbreğinde bazı antioxidant enzimlerin aktiviteleri üzerine etkisi

The effect of 2,4-dichlorophenoxyacetic acid on some antioxidant enzymes in kidneys of the second cross offsprings mice

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic herbicide used to control a wide variety of broad-leaved weeds in agriculture. But, several studies have been suggested that 2,4-D is a carcinogenic, mutagenic, clasto-genic and neurotoxic agent. The aim of the present study was to investigate the effect of 2,4-D on some kidney antioxidant enzymes activities such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the second cross offsprings (Mus musculus). Female mice were divided into two equal groups: 2,4-D group and ethanol control group. Two the chemicals used were applied intraperitoneally. 2,4-D was administrated to maternal mice as a 1/100 dilution of 338 mg/kg body weight (LD30) in 3 day intervals. In the control group it was administrated by similar way. Same processes were repeated over two generations. Spectrophotometric method was used to determine the activities of enzymes in the kidney of the second cross offsprings. The activity of CAT was found to be significantly lower in 2,4-D group compared to control ethanol group (p<0.001). There was no significant difference in the SOD and GSH-Px activities between the 2,4-D and control groups. The results obtained suggest that the CAT activity of kidney in the second cross offsprings was affected from 2,4-D toxicity.
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Abstract (Original Language): 
2,4-diklorofenoksiasetik asit (2,4-D) tarımda geniş yapraklı yabani otların kontrolünde kullanılan sistemik bir herbisittir. Fakat, bazı çalışmalar, 2,4-D'in karsinojenik, mutajenik, klastogenik ve nörotoksik bir ajan olduğunu belirtmektedirler. Bu çalışmanın amacı, 2,4-D'in ikinci çapraz yavruların (Mus musculus) böbreğinde antioksidan enzimlerden süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) enzimlerinin aktiviteleri üzerine etkilerini incelemektir. Dişi fareler, 2,4-D ve kontrol gruplarına ayrıldı. 2,4-D anne farelere LD30 dozu olan 338 mg/kg vücut ağırlığı dozundan 1/100 seyreltilerek üç günde bir intraperitoneal(i.p.) olarak verildi. Kontrol grubuna da etanol aynı yolla verildi. Bu işlemler iki nesil boyun¬ca yapıldı. Ikinci çapraz yavruların böbreğinde, enzim aktiviteleri spekrofotometrik olarak ölçüldü. CAT aktivitesi, 2,4-D grubunda, kontrol grubuna göre anlamlı olarak azaldı (p<0.001). SOD ve GSH-Px aktivitelerinde ise anlamlı bir fark gözlenmedi. Sonuç olarak, ikinci çapraz yavruların böbreğindeki CAT aktivitesinin 2,4-D'in toksisitesinden etkilendiği söylenebilir.
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  • 2
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  • Turkish

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