You are here

Home » Content

Melatoninin rat akciğer hücreleri ve eritrositlerinde 27.17 mhz radyofrekans radyasyonu ile oluşturulan lipid peroksidasyonu üzerine önleyici etkisi

Melatonin protects the formation of lipid peroxidation induced by 27.17 mhz radiofrequency radiation in rat lung cells and erythrocytes

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Introduction: A decrease in melatonin secretion has been observed in small mammals under exposure to radio-frequency radiation. As there is some concern about possible health effects of the increasing use of 27.17 MHz citizen band radios emitting radiofrequency electromagnetic fields, we examined whether such fields would alter the level of malondialdehyde (MDA) that is extensively used as a marker of lipid peroxidation. Objective: The purpose of this experiment was to determine whether the exposure of rats to pulsed direct current mag¬netic fields (MFs) would cause a lipid peroxidation in the rat lung cells. Also, the experiment was carried out to investigate whether treatment with melatonin could block the effect of magnetic fields on lung tissue. Material and methods:Thirty rats were included in the study. The animals were divided into three groups (MF and MF+melatonin, controls) equally. The animals in the MF+melatonin group were injected with melatonin (4mg/kg, intraperitoneal) and exposed to a 27.17 MHz MFs, dummy-load was 50 Ohms for matched antennas impedance. Duration of exposure was 2.5 hours/day, 6days/week for two weeks. MF group were exposed to same magnetic fields at the same times. Control rats were injected with only physiologic saline solution (1mg/kg, intraperitoneal). After the experiment, the rats were sacrificed and thoracic cavity was explored; blood samples were obtained from the cardiac region and lungs were extracted and homogenised. MDA lev¬els were studied in all groups. Results: MDA levels were significantly higher in the MF group than the MF+melatonin group, for erythrocyte hemolysate 3.53 0.64 nmol/ml vs 1.72 0.64 nmol/ml (p=0.000), and for lung tissue 2.72 0.51 nmol/gr vs 1.44 0.28 nmol/gr (p=0.000), respectively. Conclusion: Melatonin provides significant protection for lung cells and erythrocytes from oxidative damage induced by MFs exposure.
Bookmark/Search this post with
Abstract (Original Language): 
Giriş: Radyofrekans radyasyonuna maruz kalan küçük memelilerde melatonin sekresyonunda bir azalma gözlemlenmiştir. Radyofrekans elektromanyetik alanlar yayan 27.17 MHz band radyoların artan kullanımının sağlık üzerine olası etkileri hakkında endişeler olması sebebiyle bu alanların lipid peroksidasyonunun bir belirteci olarak yaygın kullanılan malondialdehid (MDA) düzeyinde değişiklik yapıp yapmadığını araştırdık. Amaç: Bu deneyde direkt manyetik alan (MA) akımına maruz kalan ratların akciğer hücrelerinde lipid per-oksidasyonun oluşup oluşmadığını ve manyetik alanların akciğer dokusu üzerindeki etkilerinin melatonin tedavisiyle engellenip engellenmediği belirlemek. Gereç ve yöntem: Çalışmaya otuz rat alındı ve hayvanlar üç gruba ( MA, MA+melatonin, kontrol) eşit olarak dağıtıldı. MA+Melatonin grubuna günlük 4mg/kg intraperitoneal melatonin enjekte edildi ve uyarlanmış anten impedansının kör-yükü 50 Ohm olan 27.17 MHz manyetik alana iki hafta boyunca, haftada altı gün, günde 2.5 saat maruz bırakıldı. MA grubu da aynı manyetik alana aynı süre maruz bırakıldı. Kontrol grubuna ise sadece 1 mg/kg fizyolojik salin solüsyonu intraperitoneal uygulandı. Deney sonunda tüm ratların toraks boşlukları açıldı. Kardiyak ponksiyon ile kan örnekleri alındı, akciğerler çıkarılarak homojenize edildi ve her üç grupta MDA düzeyleri çalışıldı. Bulgular: MDA düzeyleri eritrosit hemolizatında 3.53 0.64 nmol/ml, 1.72 0.64 nmol/ml (p=0.000) ve akciğer dokusunda 2.72 0.51 nmol/gr, 1.44 0.28 nmol/gr (p=0.000) olmak üzere MA grubunda MA+melatonin grubuna göre anlamlı olarak yüksek bulundu. Sonuç: Melatonin ratlarda MA maruziyetiyle oluşturulan oksidatif hasara karşı eritrositler ve akciğer hücreleri için anlamlı bir koruma sağlamaktadır.
FULL TEXT (PDF): 
PDF icon arastirmax-melatoninin-rat-akciger-hucreleri-eritrositlerinde-27.17-mhz-radyofrekans-radyasyonu-ile-olusturulan-lipid-peroksidasyonu-uzerine-onleyici-etkisi.pdf
  • 4
14-17
  • Turkish

REFERENCES

References: 

1- Özen Ş.
Measuremen
t of the Magnetic Field Strength Levels at
27 MHz-1000MHz Frequency Range Affects Living Organisms; Comparing of Results by Means of Invivo-Invitro Conditions, and Epidemiologic Data, Post Graduate Thesis, Isparta, 2000 .
2- IRPA. Interim Guidelines on Limits of Exposure to
Radiofrequency Electromagnetic Fields In The Frequency Range From 100kHz to 300 GHz. Health Physics 1988; 54:115-123.
3- Boorman GA, Gauger JR Johnson TR Tomlinson MJ, Findlay
JC, Travlos GS, et al. Eight-week toxicity study of 60 Hz magnetic fields in F344 rats and B6C3F1 mice. Fundam Appl Toxicol 1997; 35: 55-63.
4- Wilson BW, Matt KS, Morris JE, Sasser LB, Miller DL,
Anderson LE. Effects of 60 Hz magnetic field exposure on the pineal and hypothalamic-pituitary-gonadal axis in the Siberian
hamster (Phodopus sungorus). Bioelectromagnetics 1999; 20:
224-32.
5-Loomis DP, Savitz DA, Ananth CV. Breast cancer mortality among female electrical workers in the United States. J Natl Cancer Inst 1994; 86:921-5.
6- Gorman MR, Zucker I. Pattern of change in melatonin duration determines testicular responses in Siberian hamsters, Phodopus sungorus. Biol Reprod 1997; 56:668-73.
7- Ahlbom A. A review of the epidemiologic literature on magnetic fields and cancer. Scand J Work Environ Health 1988;14:337-43.
8-Lai H, Singh NP. Melatonin and a spin-trap compound block radiofrequency electromagnetic radiation-induced DNA strand breaks in rat brain cells. Bioelectromagnetics 1997; 18: 446-54.
9- Reiter RJ. Oxidative process and antioxidative defense mechanisms in the aging brain. FASEB J 1995; 9:526-33.
10- Reiter RJ, Tan DX, Poeggeler B, Kavet R. Inconsistent suppres¬sion of nocturnal pineal melatonin synthesis and serum mela-tonin levels in rats exposed to pulsed DC magnetic fields. Bioelectromagnetics 1998; 19:318-29.
11- Yang MH, Schaich KM. Factors affecting DNA damage caused by lipid hydroperoxides and aldehydes. Factors affect¬ing DNA damage caused by lipid hydroperoxides and aldehy¬des. Free Radic Biol Med 1996; 20:225-36.
12- Ohta Y, Kongo M, Sasaki E, Nishida K, Ishıguro I. Therapeutic effect of melatonin on carbon tetrachloride-induced acute liver injury in rats. J Pineal Res 2000; 28:119-26.
13- Brocklehurst B, McLauchlan KA. Free radical mechanism for the effects of environmental electromagnetic fields on biologi¬cal systems. Int J Radiat Biol 1996; 69:3-24.
14- IEEE. Standard for safety levels with respect to human expo¬sure to radiofrequency electromagnetic fields, 3 kHz to 300 GHz. New York: Institute of Electrical and Electronic Engineers, 1992; C95.1-1991.
15- NRPB. Electromagnetic fields and the risk of cancer. Report of an Advisory Group on Non-ionising Radiation. Chilton, UK: National Radiological Protection Board, Doc. NRPB 1992; 3:1¬138.
16- Cridland NA. Electromagnetic fields and cancer: A review of relevant cellular studies. Chilton, AL: National Radiological Protection Board, NRPB-R256, HMSO, London. 1993.
17- Verschaeve L. Can non-ionizing radiation induce cancer? Cancer J 1995; 8:237-49.
18- Sarkar S, Ali S, Behari J. Effect oflow power microwave on the mouse genome: A direct DNA analysis: Mutant Res 1994; 320:141-7.
19- Reiter RJ. Melatonin aspects of exposure to low frequency elec¬tric and magnetic fields. In: Lin JC (ed). Advances in electro¬magnetic fields in living systems. Vol 2. New York, Plenum Press, 1997; 1-27.
20- Stevens RG. Electric power use and breast cancer. Am J Epidemiol 1987; 125:294-300.
21- Boorman GA, Gauger JR Johnson TR, Tomlinson MJ, Findlay JC, Travlos GS, et al. Eight-week toxicity study of 60 Hz mag¬netic fields in F344 rats and B6C3F1 mice. Fundam Appl Toxicol 1997; 35:55-63.
22-Lary JM, Conover DL, Foley ED, Hanser PL. Teratogenic effects of 27.12 MHz radiofrequency radiation in rats. Teratology 1982; 26:299-309.

Thank you for copying data from http://www.arastirmax.com