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Myokardiyal iskemi reperfüzyon sonrası böbrek hasarında nitrik oksit'in rolü ve caffeic acid phenethyl ester (cape)'in etkisi

Role of nıtrıc oxıde ın the renal damage after myocardıal ıschemıa reperfusıon and effect of caffeıc acıd phenethyl ester (cape)

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2005

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
It is known that there is a change peripheral blood flow during myocardial ischemia-reperfusion (MI/R). This situation causes damage in the kidneys which are one of the much perfusing organs. The above mentioned damage occurring in the kidneys reduces use of myocardial revascularization and such like heart surgery. However, even though the reasons of this kidney damage are various, it has been informed that it depends on factors such as hypoperfusion, disappearing of pulsatile perfusion, hemolysis and systemic inflammatory reply during revascularization. In addition, it has been informed that it is effective in the damage that nitric oxide (NO) combines with superoxid radical (O2-) and forms peroxinitrit (ONOO-) in the kidney with ischemia reperfusion done directly. In the our study, we studied the effect of antioxidant Caffeic acid phenethyl ester (CAPE) on NO level which is thought to be related with the damage occurring kidney perfusion changes due to myocardial ischemia reperfusion (MI/R). Twenty one adult male Wistar rats were divided into tree groups as control, MI/R and CAPE+MI/R. The left coroner artery was occluded for 30 min and then reperfused for 120 min more before the experiment was terminated in the MI/R and CAPE+MI/R groups, and CAPE (50 mol kg-1) was administered 10 min prior to ischemia and during occlusion by infusion in the CAPE group. At the end of the reperfusion period, rats were sacrificed, and the kidneys were quickly removed for NO determination and histopathological analysis. Compared with control, MI/R was accompanied by a significant increase in NO production in the rat kidney. Administration of CAPE reduced NO production in the kidney. These beneficial changes in these biochemical parameters were also associated with parallel changes in histopathological appearance. Consequently, MI/R changes peripheral blood perfusion and increases NO level in the kidneys and thus, the excessive increase in NO level probably also plays a role in renal disorders during heart surgery. We can say that these harmful effects can be prevented by means of CAPE.
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Abstract (Original Language): 
Myokardiyal iskemi-reperfüzyon (MI/R) sırasında perifere giden kan akımında bir değişiklik olduğu bilinmektedir. Bu durum fazla kanlanan organlardan biri olan böbreklerde hasara neden olmakta ve oluşan bu hasar myokardiyal revaskülarizasyon ve benzeri kalp cerrahisinin yararlarını azaltmaktadır. Sözkonusu olan bu böbrek hasarın nedenleri çok çeşitli olmakla birlikte, revaskülarizasyon sırasında hipoperfüzyon, pulsatil perfüzyonun kaybolması, hemoliz ve sistemik inflamatuvar yanıt gibi faktörlerin etkili olduğu belirtilmiştir. Buna ek olarak, direkt yapılan böbrek iskemi reperfüzyonunda meydana gelen nitrik oksid (NO)'in süperoksit (O2-) radikali ile birleşerek meydana getirdiği peroksinitrit (ONOO-) bu hasarın meydana gelmesinde önemli bir role sahip olduğu bildirilmiştir. Bu çalışmada MI/R ile oluşan böbrek perfüzyon değişikliklerinde, hasarla ilişkili olduğu sanılan NO düzeyi üzerine antioksidan Caffeic acid phenethyl ester (CAPE)'in etkisi araştırıldı. Yirmi bir yetişkin erkek Wistar sıçan kullanıldı. Hayvanlar; kontrol, MI/R ve CAPE+MI/R olmak üzere üç gruba ayrıldı. MI/R ve CAPE+MI/R grubundaki sıçanların sol koroner arteri 30 dakika süreyle kapatıldı (iskemi) ve 120 dakika süreyle de tekrar açıldı (reperfüzyon). CAPE+MI/R grubunda bulunan sıçanlara iskemiden on dakika önce başlanılarak reperfüzyon başlangıcına kadar CAPE (50 îmol kg-1), perfüzyon pompasıyla uygulandı. Reperfüzyon sonrası böbrekler alınarak NO düzeyleri ve histolojik değişiklikler araştırıldı. MI/R grubu sıçanlarınböbreklerinde kontrol grubuna göre NO düzeyinde istatistiksel olarak anlamlı bir artış gözlendi (p<0.05). CAPE MI/R'ın neden olduğu böbrek NO düzeyindeki artışı önledi. Histopatolojik sonuçların da bu durumu desteklediği görüldü. Sonuç olarak, MI/R'ın periferdeki kan perfüzyonunu değiştirerek böbreklerde NO düzeyini artırdığı, kalp cerrahisinde meydana gelen böbrek bozukluklarında muhtemelen NO düzeyindeki aşırı artışın da rol oynadığını ve bu zararlı etkinlerin CAPE ile önlenebileceğini söyleyebiliriz.
FULL TEXT (PDF): 
PDF icon arastirmax-myokardiyal-iskemi-reperfuzyon-sonrasi-bobrek-hasarinda-nitrik-oksitin-rolu-caffeic-acid-phenethyl-ester-capein-etkisi.pdf
  • 4
23-27
  • Turkish

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