You are here

Home » Content

Hepatit C Virus infeksiyonu tanısında polimeraz zincir reaksiyonu ve serolojik yöntemlerinin karşılaştırılması

Comparison of polimerase chain reaction and serological methods in the diagnosis of hepatitis C virus infection

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Diagnosis of HCV infection depends on detecting anti-HCV antibody (HCV-Ab) through serologic methods or on detecting of HCV RNA with molecular methods. The aim of this study was to evaluate the use of serological methods and polymerase chain reaction (PCR) for the diagnosis of HCV infection. A total of 87 patients were studied. HCV RNA was detected by real time PCR with GeneAmp 5700 Sequence Detection System. Thirty (34.5%) of 87 patients were HCV RNA positive and 60 (69%) were HCV-Ab positive. fourteen of the HCV-Ab positive patients had low antibody titers (below 2.5 U/ml). None of these was found as HCV RNA positive. All of HCV-Ab negative patients were HCV RNA negative and all of the HCV RNA positive cases were HCV-Ab positive also. Since HCV RNA was negative in all of HCV Ab negative patients, it can be suggested that HCV-Ab detection by a reliable ELISA technique in the routine procedure is sufficient to determine HCV infection . On the other hand, low titer positive HCV-Ab cases must be restudied by ELISA, and PCR for HCV RNA confirmation. By using ELISA and PCR methods at the same time would help to confirm the positive results and early diagnosis.
Bookmark/Search this post with
Abstract (Original Language): 
Hepatit C virus (HCV) infeksiyonunun tanısı, serolojik olarak anti-HCV veya moleküler yöntemlerle HCV RNA'nın tespitiyle gerçekleştirilebilmektedir. Bu çalışmada HCV infeksiyonu tanısında polimeraz zincir reaksiyonu ve serolojik yöntemlerinin yerinin araştırılması amaçlanmıştır. Çalışmaya 87 hasta alındı. HCV RNA real time PCR (GeneAmp 5700 Sequence Detection System) ile tespit edildi. Seksenyedi hastanın 30 (%34.5)'unda HCV RNA ve 60 (%69)'ında anti-HCV pozitifliği tespit edildi. HCV antikorları pozitif hastaların 14'ünde antikor titreleri 2.5 U/ml'nin altında bulundu. Düşük titreli hastaların hiçbirinde HCV RNA pozitifliği saptanmadı. Antikoru negatif hastaların hepsinin HCV RNA'ları da negatifti. Ayrıca HCV RNA pozitif hastaların hepsinin HCV antikorları da pozitifti. HCV antikorları negatif tüm hastalarda HCV RNA'nın da negatif olması göz önünde bulundurulursa rutin tarama amaçlı işlemlerde güvenilir bir ELISA tekniği ile anti-HCV antikorunun araştırılmasının kişinin HCV virusu ile infekte olup olmadığını belirlemede yeterli olacağı düşünülebilir. Diğer yandan anti-HCV antikorları tespit edilen serumların öncelikle ELISA ile tekrar çalışılması ve hastalığın doğrulanması için HCV RNA testlerinin yapılması gerektiği düşünülmektedir. ELISA ve PCR metodlarının birlikte kullanılması pozitif sonuçların doğrulanmasına ve erken tanıya yardımcı olacaktır.
FULL TEXT (PDF): 
PDF icon arastirmax-hepatit-c-virus-infeksiyonu-tanisinda-polimeraz-zincir-reaksiyonu-serolojik-yontemlerinin-karsilastirilmasi.pdf
  • 1
10-14
  • Turkish

REFERENCES

References: 

1. Anand BS, Velez M. Assessment ofcorrelation between serum titers ofhepatitis c virus and severity ofliver disease. World J Gastroenterol 2004;10(16):2409-
1.
.A lter MJ. The epidemiology ofhepatitis C virus in the est. SeminLiv Dis 1995; 15: 5-14.
.C astro FC, Sauleda S, Esteban JI, Viladomiu L, Martell , Dragon E, Esteban R and Guardia J. Evaluation of epatitis C virus RNA RT/PCR qualitative and uantitative second generation assays. J Virol Meth
2001;91(1): 51-8.
4. Hennig H, Schlenke P, Kirchner H, Bauer I, Schulte-Kellinghaus B and Bludau H. Evaluation ofnewly developed microparticle enzyme immunoassays for the detection ofHCV antibodies. J Virol Meth 2000; 84(2): 181-90.
5. Berry N, Chakravarti A, Das U, Kar P, Das BC and Mathur MD. HCV seroreactivity and detection ofHCV RNA in cirrhotics. Diag Microbiol Infec Dis. 1999; 35(3): 209-13.
6. Kurtz JB, Boxall E, Qusir N, Shirley J, Coleman D and Chandler C. The diagnostic significance ofan assay for "total' hepatitis C core antigen. J Virol Meth 2001; 96(2):127-32.
7. Ruzzenenti MR, De Luigi MC, Bruni R, Giannini G, Bo A, Valbonesi M, Barresi R, Torretta F, Gianotti P and Bruzzone B. PCR testing for HCV in anti-HCV negative blood donors involved in the so called HCV + ve post-transfusion hepatitis. Transfusion Science 2000; 22(3): 161-4.
8. Williamson LM, Heptonstall J and Soldan K. A SHOT in the arm for safer blood transfusion. Br Med J 1996; 313:1221-2.
9. Schreiber GB, Bush MP, Kleinmann SM and Korelitz JJ. The risk oftansfasion-transmitted viral infections. N Engl J Med 1996; 334:1685-90.
10. Higuchi R, Fockler C, Dollinger G and Watson R. Kinetic PCR analysis: real-time monitoring ofDNA amplificationreactions. Biotechnology 1993; 11:1026¬30
11. Heid CA, Stevens J, Livak JK and William PM. Real time quantitative PCR. Genome Res 1996; 6:986-994.
12. Martell M, Gomez J, Esteban JI, Sauleda S, Quer J, Cabot B, Esteban R, Guardia J. High-throughput realtime reverse transcription-PCR quantitation ofhepatitis C virus RNA. J Clin Microbiol. 1999;37(2):327-32.
13. Martin P, Fabrizi F, Dixit V, Quan S, Brezina M, Kaufman E, Sra K, DiNello R, Polito A and Gitnick G. Automated RIBA Hepatitis C Virus (HCV) Strip Immunoblot Assay for Reproducible HCV Diagnosis. Journal ofClinical Microbiology 1998;36 (2):387-90.
14. Lee SR, Wood CL, Lane MJ, Francis B, Gust C, Higgs CM, Nelles MJ, Polito A, DiNello R and Achord D. Increased detection ofhepatitis C virus infection in commercial plasma donors by a third-generation screening assay. Transfusion 1995;35:845-9.
15. Mohan KV, Murugavel KG, Rajanikanth, Mathews S, Raghuram K, Rajasambandam P, Murali A, Srinivas U, Mathiazhagan, Palaniswamy KR, Panda SK, Thyagarajan SP. Diagnosis ofhepatitis C virus infection by ELISA, RIBA and RT-PCR: a comparative evaluation. IndianJGastroenterol. 1999;18(2):73-5.
16. van der Poel CL, Cuypers HTM and Reesink HW. Hepatitis C virus six years on. Lancet 1994;344:1475-
9.
S.D.Ü. Tıp pak.
Derg
. 2007:14(1)/10-14
14
Kaya, Hepetit C virüs infeksiyonu
17. de Medina M, Hill M, Sullivan HO, Leclerq B, Pennell JP, Jeffers L, Reddy KR, SchiffER, Perez GO. Detection ofanti-hepatitis C virus antibodies in patients undergoing dialysis by utilizing a hepatitis C virus 3.0 assay: correlation with hepatitis C virus RNA. J Lab ClinMed. 1998 Jul;132(1):73-5.
18. Takeuchi T, Katsume A, Tanaka T, Abe A, Inoue K, Tsukiyama-Kohara K, Kawaguchi R, Tanaka S, Kohara M. Real-time detection system for quantification of hepatitis C virus genome. Gastroenterology. 1999;116(3):763-4.
19. Shigeki M, Kayoko F, Chieko M, Rieko S, Shinichi H, Kazunori N, Kenji T and Takeo J. High-throughput HBV DNA and HCV RNA detection system using a nucleic acid purification robot and real-time detection PCR: its applicationto analysis ofposttransfusion hepatitis. Transfusion2002;42(1):100-6.
20. Sayan M, Meriç M, Mutlu B, Çelebi S, Wilke A. Low positive anti-HCV microparticle enzyme immunoassay results: do they predict hepatitis C virus infection? Mikrobiyoloji Bülteni 2006;40:81-4.

Thank you for copying data from http://www.arastirmax.com