You are here

Kronik melatonin enjeksiyonunun renin granülleri üzerindeki histolojik etkisi

Histological effect of chronically injected melatonin on the renin granules

Journal Name:

Publication Year:

Keywords (Original Language):

Abstract (2. Language): 
Many studies have reported that melatonin has a relationship with kidney functions directly or indirectly. The aim of this study was to examine the possible histological changes at renin granules in the juxtaglomerular apparatus in the kidneys after chronically injection of melatonin. Twenty six female Wistar rats were divided into two groups: control (C) group (n=11) and chronically melatonin injected (M) group (n=15). Melatonin was injected subcutaneously 1 mg/kg daily in a volume of 2 cc for 3 weeks. At the end of the experiment, serial sections of kidneys were stained with Hematoxylen-eosin, periodic acid schiff (PAS) and Van Gieson stain. In Hematoxylen-eosin and Van Gieson stained kidneys, no different histological changes was observed in both group. In the PAS stained kidney sections, an increase has been detected in the renin granules (juxtaglomerular cells), qualitatively in the M group. It is thought that this increase might be the regulatory effect of melatonin on kidneys and blood pressure.
Abstract (Original Language): 
Birçok çalışmada melatoninin böbrek fonksiyonları ile doğrudan veya dolaylı ilişkisi olduğu bildirilmiştir. Bu çalışmanın amacı kronik melatonin enjeksiyonundan sonra böbrekteki jukstaglomerüler apparatusta bulunan renin granüllerindeki olası histolojik değişiklikleri araştırmaktı. Yirmi altı adet Wistar cinsi dişi sıçan iki gruba bölünmüştür: kontrol (K) grubu (n=ll) ve kronik olarak melatonin enjekte edilen (M) grup (n=15). Melatonin 3 hafta boyunca hergün, subkutan olarak l mg/kg dozunda ve 2 cc hacimde enjekte edildi. Deneyin sonunda böbrekten alınan seri kesitler Hematoksilen-eosin, periodik asit schiff (PAS) ve Van Gieson boyaları ile boyandı. Hematoksilen-eosin ve Van Gieson ile boyanmış böbreklerde iki grup arasında herhangi bir farklılık gözlenmedi. PAS ile boyanan böbrek kesitlerinde ise M grubunda renin granüllerinde (jukstaglomerüler hücrelerde) niteliksel olarak bir artış gözlendi. Bu artışın melatoninin böbrekler ve kan basıncı üzerindeki düzenleyici etkisi sonucu oluştuğu düşünüldü.
25-28

REFERENCES

References: 

1. Nowak YZ, Zawilsha YB. Melatonin and its physiological and therapeutic properties. Pharm World Sci 1998;20:18-27.
2. Song Y, Poon AMS, Lee PPN, Pamg SF. Putative melatonin receptors in the male guinea pig kidney. JPinealRes 1993;15:153-160.
3. Drew JE, Williams LM, Hannalı LT, Barrett P, Abramovich DR. Melatonin receptors in the human fetal kidney: 2-[1 125] iodomelatonin binding sites cornelated with expression of Mel 1a and Mel 1b receptor genes. J Endocrinol
1998;156:261-7.
4. Laflamme AK, WU L, Faucart S, Champlain Y. Impaired basal sympathetic tone and alfa1 adrenergic responsiveness in association with the hypotensive effect of melatonin in spontaneously hypertensive rats. Am J Hypertens 1998;11(2): 219-2,.
5. Burgess HJ, Sletten T, Savic N, Gilbert SS, Dawson D. Effects ofbright light and melatonin on sleep propensity temperature and cardiac activity atnight.JAppl Physiol 2001;91(3):1214-
22.
6. Karppanen H, Vapaatalo H, Lahovaara S, Paasonen MK. Studies with pinealectomized rats.
Pharmacology 1970;3(2):76-84.
7. Zanoboni A, Zanoboni-Muciaccia W. Experimental hypertension in pinealectomized rats. Life Sci 1967;6:2327-31.
8. Girouard H, Chulak C, Kejossec M, Lamontahne D, Champlain Y. Vasorelexant effects of the chronic treatment with melatonin on mesenteric artery and aorta of spontaneously hypertansive rats. J Hypertens 2002;19:1369-77.
9. Esquino AI, Moreno ML, Steger RW. Effects of chronic melatonin administration on adrenal medulla catecholamine metabolism in adult male goldenhamsters. J Pineal Res 1994;16(3):154-8.
lO.Itskovitz HD, Hildreth EA, Sellers AM, Blakemore WS. The Granularity of thejuxtaglomerular cells in human, Histologic and clinical correlations. Ann Intern Med 1963;59(1):8-23.
11. Hartroft PM, HartrofWS. Studies on renal juxtaglomeruler cells, variations produced by sodium chloride and desoxy corticosterone acetate. JExpMed 1953;97:415-29.
12. Lindop GB. Morphological aspects of renin synthesis, processing, storage, and secretion. KidneyInt 1987;20:18-24.
13. Lusardi P, Preti P, Savino S, Piazza E, Zoppi A, Fogari R. Effect ofbedtime melatonin ingestion on bloodpressure ofnormotensive subjects. Blood Press Monit 1997;2(2):99-103.
14. Arangino S, Cagnacci A, Angiolucci M et al., Effects of melatonin on vascular reactivity, catecholamine levels, and blood pressure in healthy men. 1999;83(9):1417-9.
15. Kawashima K, Nagahura A, Wurzburger R, Spector S. Melatonin in serum and the pineal of spontaneuslyhypertensive rats. Clin Exp Hypertens (A) 1984;6:1517-28.
16.Sewerynek E. Melatonin and the cardiovascular system. Neuroendocrinol Lett 2002;23(1): 79-83.

Thank you for copying data from http://www.arastirmax.com