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Lipid peroksidasyonu ve deneysel kafa travması Na +-K + / ATPaz enzim aktivitesi üzerine mannitol Etkileri

Effects of mannitol on lipid peroxidation and Na+-K+/ATPase enzyme activity in experimental head trauma

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: This study was designed to determine the effects of mannitol on tissue Na+/K+-ATPase enzyme activity and malondialdehyde (MDA) levels in rabbit brain after experimental head trauma. Methods: Thirty New Zealand rabbits were divided equally into three groups: Group S (sham-operated group), group C (untreated group) and group M (mannitol treated group). Grup C and group M received head trauma with the weight drop method. Mannitol was administered 1,0 g/ kg i.v. immediately after the head trauma to group M. Head trauma was applied to the right side of the head. One hour after trauma, brain cortices were resected from both sides and the Na+/K+-ATPase enzyme activity and MDA levels were determined. Results: Na+/K+-ATPase activities were increased and MDA levels were decreased in mannitol treated rabbits but the differences were not statistically significant (p>0,05). Conclusion: Head trauma did not cause to lipid peroxidation and any changes in Na+/K+-ATPase activity in brain tissue of rabbits in this model. Mannitol treatment after the trauma does not affect these parameters.
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Abstract (Original Language): 
Amaç: Bu çalişma tavşanlarda deneysel kafa travmasindan sonra doku Na+/K+-ATPaz aktivitesi ve malondialdehyde (MDA) seviyeleri üzerine mannitolün etkilerini belirlemek için dizayn edildi. Metod: Otuz Yeni Zelanda tavşani eşit olarak üç gruba ayrildi: Grup S: Şam grubu (şam operasyonu yapilan), grup C: Tedavi edilmemiş grup, grup M: Mannitol ile tedavi edilen. Grup C ve grup M üzerine ağirlik düşürme metodu ile travma uygulandi. M grubuna, travmadan hemen sonra 1,0 g/ kg i.v. olarak mannitol uygulandi. Kafa travmasi kafatasinin sağ tarafina uygulandi. Travmadan bir saat sonra her iki taraf beyin korteksi cerrahi olarak çikarildi ve beyin dokularinda Na+/K+-ATPase enzim aktivitesi and MDA seviyeleri belirlendi. Bulgular: Mannitol verilmesiyle Na+/K+-ATPase enzim aktivitesi artarken MDA seviyeleri düşük bulundu. Ancak bu değişimler istatistiksel olarak anlamli değildi (p>0,05). Sonuç: Bu modelle oluşturulan travma tavşanlarin beyin dokularinda Na+/K+-ATPase enzim aktivitesinde ve lipid peroksidasyonunda bir değişikliğe sebep olmamiştir. Travmadan sonra uygulanan mannitol tedavisi bu parametreler üzerinde etkili olmamiştir.
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  • 3
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  • Turkish

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