You are here

Home » Content

Vibrio anguillarum Suşlarında Çevreyi Algılama Sistemi ve Virülens Faktörlerinin İncelenmesi

Investigation on Quorum Sensing System and Virulence Factors of Vibrio anguillarum Strains

Journal Name:

  • Süleyman Demirel University Journal of Eğirdir Fisheries Faculty

Publication Year:

  • 2016

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In recent years, are reported that virulence factors of many pathogenic bacteria are controlled via acyl homoserine lactone (AHL) signaling molecules. In this study, production of N-acyl homoserine lactone signaling molecules were investigated using Choromobacterium violaceum CV026 and Agrobacterium tumefaciens NT1 biosensor strains in Vibrio anguillarum isolates, Gram negative fish pathogens. Pseudomonas aeruginosa PAO1 strain was used as a positive control. As a result of, it was found that negative results by using C. violaceum CV026 in test, despite positive results using A. tumafeciens NT1 in test of V. anguillarum strains. In addition were determined of biofilm formation, production of pigments and protease activity which are thought to play an important role in the pathogenesis in the V. anguillarum strains.
Bookmark/Search this post with
Abstract (Original Language): 
Son yıllarda yapılan araştırmalar, patojenik bakterilerin bir çoğunun virülens faktörlerinin üretimini N-açil homoserin lakton (AHL) türevi sinyal molekülleri aracılığı ile kontrol ettiğini ortaya koymuştur. Bu çalışmada Gram negatif bakteriyel bir balık patojeni olan Vibrio anguillarum izolatlarında N-açil homoserin lakton türevi sinyal moleküllerinin üretimi, Chromobacterium violaceum CV026 ve Agrobacterium tumafeciens NT1 biyosensör suşları kullanılarak araştırılmıştır. Pseudomonas aeruginosa PAO1 suşu pozitif kontrol olarak kullanılmıştır. Çalışma sonucunda V. anguillarum suşlarının, C. violaceum CV026 suşu kullanılarak yapılan testlerde negatif sonuç vermesine karşın, A. tumafeciens NT1 kullanılarak yapılan testlerde, pozitif sonuçlar verdiği saptanmıştır. Ayrıca bu suşların, V. anguillarum’un patojenitesinde önemli rol oynadıkları düşünülen biyofilm oluşumu, pigment üretimi ve proteaz aktivitesi gösterdikleri tespit edilmiştir.
FULL TEXT (PDF): 
PDF icon arastirmax-vibrio-anguillarum-suslarinda-cevreyi-algilama-sistemi-virulens-faktorlerinin-incelenmesi.pdf
  • 1
49
57
  • Turkish

REFERENCES

References: 

Arda, M., 1997. Temel Mikrobiyoloji. Medisan Yayınevi, Medisan Yayın Serisi No:25, 490s. Ankara.
Baskın, H., 2005. Mikroorganizmanın Çevreye Uyumu ve Biyofilm: "Quorum Sensing" (Çoğunluğu Algılama). Klimik 2005 XII. Türk Klinik Mikrobiyoloji ve İnfeksiyon Hastalıkları Kongresi. Dokuz Eylül Üniversitesi Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İzmir, 9-10.
Boşgelmez-Tınaz, G., 2003. Quorum Sensing in Gram-Negative Bacteria. Turk J Biol. 27, 85-93.
Bosgelmez-Tınaz, G. “Disruption of Bacterial cell-to-cell communication (Quorum Sensing): A Promising Novel Way to Combat Bacteria-Mediated Diseases”. Journal of Marmara University Institute of Health Sciences. 3 (3), 159-163 (2013).
Boyen, F., Eeckhaut, V., Van Immerseel, F., Pasmans, F., Ducatelle, R., Haesebrouck, F., 2009. Quorum sensing in veterinary pathogens: Mechanisms, clinicalim portance and future perspectives. Veterinary Microbiology. 135, 187-195.
Bruhn, J. B, Dalsgaard, I.,Nielsen, K. F., Buchholtz, C., Larsen J. L., Gram L., 2005. Quorum sensing signal molecules (acylatedhomoserine lactones) in Gram-negative fish pathogenic bacteria. Diseases of Aquatic Organisms. (65) 43-52.
Nurcan vd. 2016 ESUFD 12(1), 49-57
56
Cha, C.,Gao, P., Chen, Y.C., Shaw, P.D., Farrand, S.K., 1998. Production of acyl homoserine lactone signals by gram-negative plant-associated bacteria. Mol Plant-Microbe Interact. (11) 1119-1129.
Chu, T.,Ni, C., Zhang, L., Wang Q., Xiao J., Zhang, Y., andLiu, Q., 2015. A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories. (14), 37
Croxatto, A.,Chalker, V.J., Lauritz, J., Jass, J., Hardman, A., Williams, P., Camara, M., Milton D.L., 2002. VanT, a Homologue of Vibrio harveyi LuxR, Regulates Serine, Metalloprotease, Pigment and Biofilm Production in Vibrio anguillarum. Journal of Bacteriology. 184(6), 1617-1629.
Deepa, S.,Venkateswaran, P., Vinithkumar N. V., Kirubagaran R., 2014. Prevention of Acyle Homoserine Lactone (AHL) mediated biofilm Formation by selected flora of Andaman & Nicobar Island. Indian Journal of Geo-Marine Sciences. (43),11.
Defoirdt, T.,Boon, N., Bossier, P., Verstraete, W., 2004. Disruption of bacterial quorum sensing: an unexplored strategy to fight infections in aquaculture. Aquaculture. 240, 69-88.
Dong, Y.,Zhang, X., Soo, H.L., Greenberg, P., Zhang, L., 2005. Thetwo-component response regulator PprB modulates quorum-sensing signal production and global gene expression in P. aeruginosa. Molecular Microbiology. 56: 1287-1301.
McClean, K.H.,Winson, M.K., Fish, L., Taylor, A., Chhabra, S.R., Camara, M., Daykin, M., Lamb, J.H., Swift, S., Bycroft, B.W., Stewart, G.S.A.B., Williams, P., 1997. Quorum sensing and Chromobacterium violeceum: exploitation of violacein production for the detection of N-acyl homoserine lactones. Microbiology. 143, 3703-3711.
Milton, D. L.,Chalker, V. J., Kirke, D., Hardman, A., Camara, M. and Williams, P. 2001. The Lux M homologue VanM from Vibrio anguillarum direct sthesynthesis of N-(3-hydroxyhexanoyl) homoserine lactone and N hexanoyl homoserine lactone. J Bacteriol. 183, 3537-47.
Milton, D.L.,Hardman, A., Camara, M., Chhabra, S.R., Bycroft, B.W., Stewart, G.S.A.B., Williams, P., 1997. Quorumsensing in Vibrio anguillarum: characterization of the vanI/vanR locus and identification of the autoinducerN-(3-oxodecanoyl)-l-homoserine lactone. J. Bacteriol. 179, 3004-3012.
Moghaddam, M. M.,Khodi S., Mirhosseini, A. 2014. Quorum Sensing in Bacteria and a Glance on Pseudomonas aeruginosa., Clinical Microbiology: Open Access. 3 (4), ISSN: 2327-5073.
Myszka, K. and Czaczyk K., 2012. N-Acyl homoserine Lactones (AHLs) as Phenotype Control Factors Producedby Gram – Negative Bacteria in Natural Ecosystems. Pol. J. Environ Stud. 21(1), 15-21. O’Toole G.A. and Kolter R. 1998. Initiation of biofilm formation in Pseudomonas fluorescensWCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Molecular Microbiology. 28(3), 449 – 461.
Purohit, A.A., 2013. Acyl homoserine lactone signaling in members of the Vibrionaceae family. University of Tromsøuit.
Rasch, M.,Buch, C., Austin, B., Slierendrecht, W.J., Ekmann, K.S., Larsen, J.L., Johansen, C., Riedel, K., Eberl, L., Givskov, M., Gram, L., 2004. An İnhibitor of Bacterial Quorum Sensing Reduces Mortalities Caused by Vibriosis in Rainbow Trout (Oncorhynchus mykiss, Walbaum). System. Appl. Microbiol. 27, 350-359.
Ravn, L.,Christensen, A.B., Molin, S., Givskov, M., Gram, L., 2001. Methods for acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-productionk inetics. Journal of Microbiological Methods. 44: 239-251.
Saraçlı, M.A., 2006. "Quorum sensing": mikroorganizmalar iletişim mi kuruyor?. Gülhane Tıp Dergisi. 48(4), 244-250.
Shaw, P.D.,Ping, G., Daly, S.L., Cha, C., Cronan, J.E., JR., Rınehart, K.L., and Farrand, S.K., 1997. Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc. Natl. Acad. Sci. USA. 94, 6036-6041.
Nurcan vd. 2016 ESUFD 12(1), 49-57
57
Swift, S., Lynch, M.J., Fish, L., Kirke, D.F., Tomas, J.M., Stewart, G.S.A.B. and Williams, P., 1999. Quorum Sensing-Dependent Regulation and Blockade of Exoprotease Production in Aeromonas hydrophila. Infection and Immunity. 67 (10), 5192-5199.
Ulusoy, S., 2007. Yoğun Bakım Ünitelerinden İzole Edilen Pseudomonas aeruginosa Suşlarında N-Açil Homoserin Lakton Üretiminin Araştırılması. Doktora Tezi, Süleyman Demirel Üniversitesi, 100s, Isparta.
Wang, L., 2004. Autoınducer-2 (Aı-2) Mediated Quorum Sensing In Escherichia coli., Dissertation submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirement forthe degree of Doctor of Philosophy Advisory Committee Thesis.
Ye, J.,Ma, Y., Liu, Q., Zhao, D.L., Wang, Q.Y., Zhang, Y.X., 2008. Regulation of Vibrio alginolyticus virulence by the LuxS quorum-sensing system. Journal of Fish Diseases. 31, 161-169.

Thank you for copying data from http://www.arastirmax.com