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Thiacloprid ve D-Tubokurarin’in Kurbağa İskelet Kası Üzerine Ultrastrüktürel Etkileri

Ultrastructural Effects of Thiacloprid and D-Tubocurarine on Frog Skeletal Muscle

Journal Name:

  • Süleyman Demirel University Journal of Eğirdir Fisheries Faculty

Publication Year:

  • 2016

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In this study, the ultrastructural alterations of neonicotinoid insecticide thiacloprid and its antagonist d-tubocurarine on skeletal muscle of Pelophylax ridibundus were investigated. Gastrocnemius muscle was isolated after frogs were decapitated. Muscle tissue was exposed to thiacloprid solution at four different concentrations (1×10-3, 1×10-4, 1×10-5 and 1×10-6 M) for 120 minutes. In addition, 1x10-4 M d-tubocurarine 1x10-5 and 1x10-5 M thiacloprid mixture as well as mixture of d-tubocurarine and 1x10-6 M thiacloprid were applied for 120 minutes on muscle tissue. Muscle tissue in the control group was maintained in Ringer's solution for 120 minutes. The agonist and antagonist effects were studied in all experimental groups with equal number of subjects (n = 3). The results of histological examinations performed by electron microscopy did not reveal any signs of degeneration on the control group. It was determined that the high level exposure as 10-3 and 10-4 M of thiacloprid was resulted in the breakdown of sarcomere integrity of muscle tissue, separation of the myofibrils and swelling of mitochondrion. Milder signs of degeneration were observed in the other dose groups.
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Abstract (Original Language): 
Bu çalışmada, neonikotinoid bir insektisit olan thiacloprid ve bir neonikotinoid insektisit antagonisti olan d-tubokurarin’in Pelophylax ridibundus iskelet kası üzerine meydana getirdiği ultrastrüktürel değişiklikler incelendi. Kurbağalar spinal hale getirildikten sonra gastrokinemius kası izole edilmiştir. Kas doku 120 dakika boyunca 1×10-3, 1×10-4, 1×10-5 ve 1×10-6 M olmak üzere dört farklı konsantrasyonda thiacloprid çözeltisi ile muamele edildi. Ayrıca, kas dokuları üzerine 1x10-4 M d-tubokurarin ile 1x10-5 M thiacloprid karışımı ve 1x10-5 M d-tubokurarin ile 1x10-6 M thiacloprid karışımı 120 dakika süresince uygulandı. Kontrol grubundaki kas dokuları 120 dakika boyunca Ringer çözeltisinde bekletildi. Tüm deney gruplarında agonist ve antagonistin etkileri eşit sayıda denek üzerinde çalışıldı (n=3). Elektron mikroskobu ile yapılan histolojik incelemeler sonucunda, kontrol grubunda herhangi bir dejenerasyon bulgusuna rastlanmadı. Thiacloprid’in 10-3 ve 10-4 M olarak uygulanan yüksek dozlarında, kas dokusunun sarkomer bütünlüğünün bozulduğu, miyofibrillerin ayrıldığı ve mitokondriyonların şiştiği tespit edildi. Diğer doz gruplarında ise daha hafif düzeyde dejenerasyon bulguları gözlendi.
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128
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  • Turkish

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