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Hemodiyaliz Hastalarında Farklı Diyaliz Membranlarının Polimorfonükleer Lökosit Oksidatif Stresi Üzerine Etkisi

Effect of Different Dialyzer Membranes on the Oxidative Burst Status of Poylymorphonuclear Leukocytes in Hemodialysis Patients

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
In this study, we aimed to investigate whether there is an abnormality in intracellular antioxidant enzyme activities of polymorphonuclear leukocytes (PMNL) of end-stage renal disease (ESRD) patients who had been treated with hemodialysis and how do the different types of dialyzer membranes affect these enzyme activities. Fifteen ESRD patients undergoing hemodialysis treatment were enrolled into the study and they were dialyzed with polysulphone (P) and cupropha-ne (C) membranes in two consecutive hemodialysis sessions. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in PMNLs from the venous blood samples of the patients, obtained before and after each hemodialysis session, were determined. A control group consisting of 15 healthy subjects was also included. Predialysis PMNL SOD (p-0.285 for P-dialysİs, p=0.512 for C dialysis) and GSH-Px (p=0.285 for P-dialysis, p=0.486 for C-dialysis) activities of the patients were not different from those of the controls. Similiarly, postdialysis PMNL SOD (p=0.838 for P-dialysis, p-0.744 for C-dialysis) and GSH-Px |p=0.512 for P-dialysis, p=0.870 for C-dialysis) activities of the patients did not show difference from control data. Hemodialysis with both types of membranes did not cause a statistically significant change in PMNL SOD (p=0.460 for P-dialysis, p-0.532 for C-dialysis) and GSH-Px (p=0.773 for P-dialysis, p=0.570 for C-dialysis} activities. In conclusion, there is no difference between ESRD patients undergoing hemodialysis and controls with respect to PMNL oxidative stress status.
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Abstract (Original Language): 
Bu çalışmada, son dönem böbrek yetmezlikli (SDBY) hemodiyaliz hastalarının nötrofil (PMNLI intraselüler antioksidan enzim aktivi-teleri ve farklı diyaliz membranlarının bu enzimler üzerindeki etkilerinin incelenmesi amaçlanmıştır. Çalışmaya SDBY mevcut 15 hasta ve kontrol grubu olarak 15 sağlıklı gönüllü dahil edilmiştir. Hastalar po-lisülfon (P) ve kuprofan (C) membranları ile diyalize alınmış, her bir diyaliz öncesi ve sonrası, venöz kanda nötrofil süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) aktiviteleri saptanmıştır. Hastaların prediyaliz PMNL SOD (p=0.285 P-diyaliz, p=0.512 C-diyaliz) ve GSH-Px (p=0.285 P-diyalîz, p=0.486 C-diyaliz) aktiviteleri ile post-diyaliz PMNL SOD {p=0.838 P-diyaliz, p=0.744 C-diyaliz) ve GSH-Px (p=0.512 P-diyaliz, p=0.870 C-diyaliz) aktiviteleri kontrol grubundan farklı bulunmamıştır. Farklı hemodiyaliz membranları kullanımıyla PMNL SOD (p=0.460 P-diyaliz, p-0.532 C-diyaliz) ve GSH-Px (p=0.773 P-diyaliz, p=0.570 C-diyaliz) aktivitelerinde, diyaliz sonrası dönemde diyaliz Öncesi döneme göre istatistiksel olarak belirgin bir farklılık saptanmamıştır. Sonuç olarak, son dönem böbrek yetmezlikli hemodiyaliz hastalarının PMNL oksidatif stres durumları kontrol vakaları-nınkinden farklı değildir. Bunun yanında, sentetik (polisülfon) veya selüloz (kuprofan) membran kullanımı, PMNL SOD ve GSH-Px enzim aktivitelerini etkilememektedir.
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  • 1
26-29
  • Turkish

REFERENCES

References: 

[. Cohen G, I laag-Weber M, I fori Wl i: Immune dysfunction in uremia, kidney [nt 1997 (Suppl); 62:S79-«2.
2. IIaag-Welx.T M, I lorl WH: Dysfunction of polymorphonuclear leukocytes in uremia. Semin Nephrol 1996;16:192-201.
3. Mailloux LU, Bellucci AG, Wilkes BM, Napolitano B, Mossey RT, Lesser M. Bluestonc PA: Mortality in dialysis patients: analysis of the causes of the dertli. Anı j kidney Dis 1991;18:326-335.
4. Sharma A, Tripatlıi AK. Kalra OP, Chakrabatry AK: Impaired function of neutrophils in uraemic patients. Natl Med J India 2000;13:121-124.
5. Hirabayashi Y, Kobayashi T, Nishikawa A, Okazaki H, Aoki T. Takaya J: Oxidative metabolism and phagocytosis of polymorphonuclear leukocytes in patients with chronic renal failure. Nephron 1988;49:305-312.
6. Otsubo 11, Kaito K, Shiba k: Plow cytometric analysis on neutrophil iniraceIkılar enzyme activity in patients on hemodialysis and continuous ambulatory peritoneal dialysis. Kansenshogaku Zass-hi 2000;74:73-81.
7. Pawlicki L, Trznadel K, kedziora J, Blaszczyk J, Sibinska E, Luci-ak M: Generation of active oxygen compounds by whole blood granulocytes in patients with chronic renal failure. Pol Arch Med Wewn I91>1;86:94-100.
8. Porter CJ, Burden Ki'. Morgan AG, Daniels I, Fletcher J: Imparled
bacterial killing and hydrogen peroxide production by polymorphonuclear neutrophils in end-stage renal failure. Nephron 1997;77:479-481.
9. Porter CJ. Burden RP, Morgan AG, Daniels 1, Fletcher J: Imparled polymorphonuclear neutrophil function in renal failure and its correction by continuous ambulatory peritoneal dialysis. Nephron 1995;71:133-137.
10. Swirski RS, Mashiach E, Kristal B, Sholnik T, Shasha SM: Antioxidant enzymes activity in polymorphonuclear leukocytes in chronic renal failure. Nephron 1995;71:176-179.
11. Vanlioider R, Dell'Aquilla R, Jacobs V, Dhondt A, Veys N, Water-loss MA, Van Land.schoot N, Van Biesen W, Ringoir S: Depressed phagocytosis in hemodialyzed patients: in vivo and in vitro mechanisms. Nephron 1993;63:409-415.
12. Eckardt KU, Eckardt H, Harber MJ, Asscher AW: Analysis of polymorphonuclear leukocyte respiratory burst activity in uremic patients by using whole blood chemiluminescence. Nephron 1986;43:274-278.
13. Jacobs AA Jr, Ward RA, Wellhausen SR. McLeish KR: Polymorphonuclear leukocyle function during hemodialysis: relationship to complement activation. Nephron 1989;52:119-124
14. Klein JB, McLeish KR, Ward RA: Transplantation, not dialysis, corrects azotemia-dependent priming of the neutrophil oxidative burst. Am J Kidney Dis 1999;33:483-491.
15- Lucchi L, Cappelli G, Acerbi MA, Spattini A, Lusvarghi E: Oxidative metabolism of polymorphonuclear leukocytes and serum opsonic activity in chronic renal failure. Nephron 1989;51:44-50.
16. Rhee MS, McGoldrick MD, Meuwissen HJ: Serum factor from patients with chronic renal failure enhances polymorphonuclear leukocyte oxidative metabolism. Nephron 1986;42:6-13.
17. Schettler V, Wieland E, Methe H, Schuff-Werner P, Müller GA: Oxidative stress during dialysis: effect on free radical scavenging enzyme (FRSE) activities and glutathione (GSF0 concentration in granulocytes, Nephrol Dial Transplant 1998;13:2588-2593.
18. Takahashi K, Imada A: Phagocytic activity and oxygen radicals production of neutrophils in patients with chronic renal failure. Nippon Jinzo Gakkai Shi 1991;33:565-574.
19. Abrutyn E, Solomons NW, Si Clair L, MacGregor RR, Root Rk: Granulocyte function in patients with chronic renal failure: surface adherence, phagocytosis, and bactericidal activity İn vitro. J Infect Dis 1997;135:1-8.
20. PaulJL, Roch-Arveiller M, Man NK, Luong N, Moatti N, Raiehvarg D: Influence of uremia on polymorphonuclear leukocyte oxidative metabolism, tn end-stage renal disease and dialyzed patients, Nephron 1991;57:428-432.
21. Nagaİ T, Kuriyama M, Kawada Y: Oxidative metabolism of polymorphonuclear leukocytes in continuous ambulator)' peritoneal dialysis. Perit Dial Int 1997;17:167-174.
22. Ward RA, McLeish KR: Polymorphonuclear leukocyte oxidative burst is enhanced in patients with chronic renal insufficiency. J Am Soc Nephrol 1995;5:1697-1702.
23. Aydın A, Orhan H, Sayal A, Özata M, Şahin G, Isımer A: Oxidative stress and nitric oxide related parameters in type II diabetes mellflus: effects of glycemic control. Clin Biochem 2001;34:65-70.
24. Jendryczko A, Grzeszczak W: Releasing activity of superoxide radicals by neutrophils and monocytes in hemodialyzed patients with chronic renal failure. Pol Arch Med Wewn 1994; 92:475-482.

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