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Hemodiyaliz Hastalarının Polimorfonükleer Lökositlerinin Oksidatif Stresi Üzerine Farklı Diyalizör Membranlarının Etkileri

Effect of Different Dialyzer Membranes on the Oxidative Burst Status of Polymorphonuclear Leukocytes in Hemodialysis Patients

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Abstract (2. Language): 
In the present study, it was aimed to investigate whether there is an abnormality in intracellular antioxidant enzyme activities of polymorphonuclear leukocytes (PMNL) of end-stage renal disease patients who had been treated with hemodialysis and different types of diaiyzer membranes (cuprophane and polysulphone) affect these enzyme activities. Fifteen end-stage renal disease patients undergoing hemodialysis treatment were enrolled into the study and they were dia-lyzed with polysulphone (P) and cuprophane (C) membranes in two consecutive hemodialysis sessions. Superoxide dismu-tase (SOD) and glutathione peroxidase (GSH-Px) activities in PMNLs before and after each hemodialysis session were determined. A control group was also consisted of 15 healthy subjects to perform the comparisons. Predtalysis PMNL SOD (p=0.285 for P dialysis, p=0.512 for C dialysis) and GSH-Px {p=0.285 for P dialysis, p=0.486 for C dialysis) activities of the patients were not different from those of the controls. Similarly, postdialysis PMNL SOD (p=0.838 for P dialysis, p=0.744 for C dialysis) and GSH-Px (p=0.512 for P dialysis, p=0.870 for C dialysis) activities of the patients did not show difference from the data of the controls. Hemodialysis with both types of the membranes did not cause a statistically significant change in PMNL SOD (p=0.460 for P dialysis, p=0.532 for C dialysis) and GSH-Px (p=0.773 for P dialysis, p=0.570 for C dialysis) activities. In conclusion, there is no difference between end-stage renal disease patients under the hemodialysis treatment and the controls with respect to PMNL oxidative stress status. Furthermore, it also seems that both synthetic (polysulphone) and cellulose (cuprophane) membranes have no effect on SOD and GSH-Px enzyme activities in the PMNLs of the hemodialysis patients.
Abstract (Original Language): 
Bu çalışmada, hemodiyaliz tedavisi gören son dönem böbrek yetmezliği hastalarında farklı diyaliz membranı (kuprofan ve polisül-fon) kullanımının polimorfonükleer lökosİTlerde (PMNL) hücre içi_an-tioksidan enzim aktiviteleri üzerindeki etkilerinin ve anormalliklerinin araştırılması amaçlanmıştır. Çalışmaya hemodiyaliz tedavisi gören 15 son donem böbrek yetmezliği hastası dahil edilmiş ve birbirini takip eden iki hemodiyaliz seansında bir kez kuprofan (C) ve bir kez de polisülfon (P! membran ile diyalize alınmışlardır. Her bir hemodiyaliz öncesi ve sonrası PMNL süperoksit dismu-taz (SOD) ve glutatyon peroksidaz (GSH-Px) aktiviteleri saptanmıştır. Ayrıca karşılaştırmalar için 15 sağlıklı bireyden bir kontrol grubu oluşturulmuştur. Hastaların hemodiyaliz öncesi PMNL SOD (p=0.285 P diyalizi, p-0.512 C diyalizi} ve GSH-Px (p=0.285 P diyalizi, p-0.486 C diyalizi) aktiviteleri kontrol grubundan farklı bulunmamıştır. Benzer olarak, hastaların hemodiyaliz sonrası PMNL SOD (p=0.838 P diyalizi, p=0.744 C diyalizi) ve GSH-Px (p=0.512 P diyalizi, p=0.870 C diyalizi) aktiviteleri de kontrol grubundan farklı bulunmamıştır. Her iki tür membran ile uygulanan hemodiyaliz PMNL SOD (p=0.460 P diyalizi, p=0.532 C diyalizi) ve GSH-Px (p=0.773 P diyalizi, p=0.570 C diyalizi) aktivitelerinde istatistiksel olarak anlamlı bir değişikliğe neden olmamıştır. Sonuç olarak, son dönem böbrek yetmezlikli, hemodiyaliz tedavisi gören hastalar ve kontroller arasında PMNL oksidatif stresi açısından bir fark yoktur. Ayrıca, hemodiyaliz hastalarında sentetik (polisülfon) ve selüloz (kuprofan) membran kullanımı PMNL SOD ve GSH-Px enzim aktiviteleri üzerinde farklılığa neden olmamaktadır.

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