You are here

Deneysel Nefrotik Sendromda Plazma ve Böbrek Dokusunda Oksidatif Stres Durumu

Oxidative Stress Status in Renal Tissue and Plasma of Experimental Nephrotic Syndrome

Journal Name:

Publication Year:

Abstract (2. Language): 
In the present study, oxidative system status both in plasma and renal tissue of rats with adriamycin induced experimental rat nephrotic syndrome was investigated. Glutathion peroxidase (GSH-Px) activities and malondialdehyde (MDA) levels in plasma and MDA levels, GSH-Px and superoxide dismutase (SOD) activities in kidney homogenates were determined. Plasma MDA levels and tissue SOD activities were significantly higher in nephrotic rats when compared to those of the controls. No correlation was found between plasma and tissue MDA levels while plasma MDA levels of nephrotic rats had positively significant correlations with urinary protein excretion and serum total cholesterol levels. Serum albumin levels showed negatively significant correlations with plasma MDA levels in nephrotic rats. The data suggest that increased oxidative stress in renal tissue seems not to be responsible principally for high plasma MDA levels observed in rats with nephrotic syndrome. Nephrotic state has significant contributions to elevated plasma MDA levels and severity of nephrotic syndrome is related to the high levels of plasma MDA.
Abstract (Original Language): 
Bu çalışmada, adriamisin ile oluşturulan deneysel sıçan nefrotik sendromunda plazma ve böbrek dokusunda oksidatif sistemin durumu araştırılmıştır. Plazma glutatiyon peroksidaz (GSH-Px) aktivitesi ve malondialdehit (MDA) düzeyleri ile böbrek homoje-natlarında MDA düzeyleri, GSH-Px ve süperoksit dizmutaz (SOD) aktiviteleri incelenmiştir. Plazma MDA düzeyleri ve doku SOD aktiviteleri kontroller ile karşılaştırıldığında nefrotik sıçanlarda belir¬gin olarak daha yüksektir. Doku ve plazma MDA düzeyleri arasında belirgin bir ilişki saptanamazken, nefrotik sıçanlarda üriner protein atılımı ve serum total kolesterol düzeyleri ile plazma MDA düzeyleri belirgin bir pozitif korelasyon içindedir. Nefrotik sıçanlarda serum albümin düzeyleri plazma MDA düzeyleri ile belirgin negatif kore¬lasyon göstermiştir. Bu bulgular, nefrotik sendromlu sıçanlarda saptanan yüksek MDA düzeylerinin esas sorumlusunun artmış renal doku oksidatif stresi olmadığını göstermektedir. Nefrotik durum yüksek plazma MDA düzeylerine belirgin olarak etki ederken, nefrotik sendromun ağırlığı ile plazma MDA düzeylerinin yüksekliği ilşkilidir
63-68

REFERENCES

References: 

1. Halliwell, B. The role of oxygen radicals in human disease
with particular reference to the vascular system.
Haemostasis 1993;23:118-126.
2. Armstrong, D. eds. Free radicals in diagnostic medicine. New
York: Plenum Press, 1994.
3. Halliwell, B. Free radicals, antioxidants and human disease:
Curiosity, cause or consequence. Lancet 1994;344:721-724.
4. Betteridge, D. J. What is oxidative stress? Metabolism
2000;2(Suppl 1):1-9.
5. Lefer, D. & Granger, D. N. Oxidative stress and cardiac disease.
Am J Med 2000;109:315-323.
6. Mccard, J. M. The evolution of free radicals and oxidative
stress. Am J Med 2000;108:652-659.
7. Alfrey, A. C. Role of iron and oxygen radicals in the progression
of chronic renal failure. Am J Kidney Dis
1994;23:183-187.
8. Ichikawa, M. Renal antioxidant enzymes: Their regulation
and function. Kidney Int 1994;45:1-9.
9. Rajbala, A., Sane, A. S., Zope, J., Mishra, V. V.& Trivedi, H. L.
Oxidative stress status in children with nephrotic syndrome.
Panminerva Med 1997;39:165-168.
10. Turi, S., Nemeth, I., Torkos, A., Saghy L., Varga I.,
Matkovisc B. & Nagy J. Oxidative stress and antioxidant
defence mechanism in glomerular diseases. Free Radic Biol
Med 1997;22:161-168.
11. Das, U. N., Mohan, I. K.& Raju, T. R. Effects of corticosteroids
and eicosapentaenoic acid/docosahexaenonic acid
on pro-oxidant and antioxidant status and metabolism
of essentially fatty acids in patients with glomerular disorders.
Prostaglandins Leukot Essent Fatty Acids
1997;65:197-203.
12. Fydryk, J., Jacobson, E.& Kurzawska, O., Malecka G., Gonet
B., Urasinski T., Brodkiewicz A. & Bukowska H. Antioxidant
status of children with steroid sensitive nephrotic syndrome.
Pediatr Nephrol 1998;12:751-754.
13. Baynes, J. W.& Thrope, S. R. Role of oxidative stress in diabetic
complications. Diabetes 1999;48:1-9.
14. Mocan, H., Aksoy, A., Uydu, H. A.& Mocan, M. C.
Oxidative damage of erythrocyte membrane in nephrotic
syndrome. Pediatr Nephrol 1999;13:326-332.
15. Bulucu, F., Vural, A., Ayd›n, A.& Sayal, A. Oxidative stress
status in adults with nephrotic syndrome. Clin Nephrol
2000;53:169-173.
16. Kinra, S., Rath, B. & Kabi, B. C. Indirect quantification
of lipid peroxidation in steroid responsive nephrotic syndrome.
Arch Dis Child 2000;82:76-78.
17. Kuroda, M., Asaka, S., Tofuku, Y.& Takeda, R. Serum antioxidant
activity in uremic patients. Nephron 1985;41:293-298.
18. Adler, S. Baker, P. J., Johnson, R. J., Ochi, R. F., Pritzl, P.&
Couser, W. G. Complement membrane attack complex stimulates
production of reactive oxygen metabolites by cultured
rat mesangial cells. J Clin Invest 1986;39:762-767.
19. Ayd›n, A., Orhan, H., Sayal, A., Özata, M., Sahin, G.& Is›mer,
A. Oxidative stress and nitric oxide related parameters in
type II diabetes mellitus: effects of glycemic control. Clin
Biochem 2001;34:65-70.
20. Richard, M. J., Arnaud, J., Jurkowitz, C., Hachache T., Meftahi
H., Laporte F., Foret M., Favier A. & Cordonnier D. Trace elements
and lipid peroxidation abnormalities in patients with
chronic renal failure. Nephron 1991;57:10-15.
21. Podracka, L., Sasinka, M., Racz, O., Sipulova, A. & Boor, A.
Antioxidant enzyme activity in erythrocytes in children with
kidney diseases. Cas Lek Cesk 1996;15:313-316.
22. Zima, T., Tesar, V., Rychlik, I., Nemecek K., Poledne R.,
Teminova J., Stipek S. & Merta M. The influence of
pefloxacine on experimental adriamycin-induced nephrotic
syndrome in rats. Ren Fail 1996;18:195-199.
23. Zima, T., Tesar, V., Rychlik, I. Nemecek K., Poledne R.,
Teminova J., Stipek S.& Merta M. The influence of
cyclosporine on lipid peroxidation and superoxide dismutase
in adriamycin nephropathy in rats. Nephron
1997;75:464-468.
24. Horie, K., Miyata, T.& Maeda, K., Miyata S., Sugiyama S.,
Sakai H., Van Ypersole De Striohu C., Monnier V.M., Witztum
J.L. & Kurokawa K. Immunohistochemical colocalization of
glycoxidation products and lipid peroxidation products in
diabetic renal glomerular lesions: Implication for glycoxidative
stress in the pathogenesis of diabetic nephropathy. J Clin
Invest 1994;15:2995-3004.
25. Zima, T., Tesar, V., Crkovska, J., Stejskalova A., Platenik J.,
Teminova J., Nemecek K., Janebova M., Stipek S. ICRF-187
(Dexrazoxan) protects from adriamycin-induced nephrotic
syndrome in rats. Nephrol Dial Transplant 1998;13:1975-
1979.
26. Van Den Branden, C., Ceyssens, B., De Craemer, D.,
Pauwels M., Vanden Houte K., De Bleser P., Hellemans K.,
Geerts A. & Verbeelen D. Renal antioxidant enzymes and
fibrosis-related markers in the rat adriamycin model.
Nephron 2000;86:167-175.
27. Warwick, G. L., Waller, H. & Ferns, G. A. Antioxidant
vitamin concentrations and LDL oxidation in nephrotic
syndrome. Ann Clin Biochem 2000;37:488-491.
28. Zachwieja, J., Bobkowski, W., Niklas, A., Strzykala, K.&
Maciejewski, J. Total antioxidant status in children with
nephrotic syndrome. Pol Merkuriusz Lek 2000;8:216-217.
29. Dogra, G., Ward, N.& Croft, K. D., Mori T. A., Barret P.H.,
Herrmann S. E., Irish A. B. Watts G. Oxidant stress in
nephrotic syndrome: Comparison of F(2)-isoprostanes and
plasma antioxidant potential. Nephrol Dial Transplant 2001;16:1626-1630.
30. Skrzep-Poloczek, B., Tomasik, A., Tarnawski, R., Hyla-Klekot
L., Dyduch A., Wojciechowska C., Wesolowski W.,
Kopieczna-Grzebieniak E.& Zalejska-Fiolka J. Nephrotic origin
hyperlipidemia, relative reduction of vitamin E level and
subsequent oxidative stress may promote atherosclerosis.
Nephron 2001;89:68-72,.
31. Bertani, T., Poggi, A., Pozzoni, R., F., Deliani F., Sacchi G.,
Thoua Y., Mecca G., Remuzzi G.& Donati M.B. Adriamycininduced
nephrotic syndrome in rats: Sequence of pathologic
events. Lab Invest 1982;46:16-23.
32. Hall, R. L., Wilke, W. L.& Fettman, M. J. The progression of
adriamycin-induced nephrotic syndrome in rats and the
effect of captopril. Toxicol Appl Pharmacol 1986;82:164-174.
33. Wang, Z., Wang, Z. G. & Liu, Z. Changes of glomerular fixed
anionic charge sites in adriamycin nephrosis in rats. Chin
Med J 1991;104:128-131.
34. Pedraza-Chaverri, J., Arevalo, A. E., Hernandez-Pando, R. &
Larriva-Sahd, J. Effect of dietary antioxidants on purumycin
aminonucleoside nephrotic syndrome. Int J Biochem Cell
Biol 1995;27:683-691.
35. Raats, C. J., Bakker, M. H., Van Den Born, J. & Berden, H. M.
Hydroxyl radicals depolymerize glomerular heparan sulfate
in vitro and in experimental nephrotic syndrome. J Biol
Chem 1997;272(42):26734-26741.
36. Ohtake, T., Kimura, M., Nishimura, M. & Hishida, A. Role of
reactive oxygen species and antioxidant enzymes in
murine daunomycin induced nephropathy. J Lab Clin Med
1997;129:81-88.
37. Korsetz, Z., Pomeranz, A., Golan, E.& Berheim, J. Pefloxacin
in adriamycin induce nephrotic syndrome in the rat. Nephrol
Dia. Transplant 1998;13:1975-1979.
38. Thabrew, M. I., Samarawickrema, N., Chandrasena, L. G. &
Jayasekera, S. Effect of oral supplementation with vitamin E
on the oxido-reductive status of red blood cells in
normal mice and mice subject to oxidative stress by chronic
administration of adriamycin. Ann Clin Biochem
1999;36:216-220.
39. Thabrew, M. I., Samarawickrema, N., Chandrasena, L. G. &
Jayasekera, S. Protection by garlic against adriamycin
induced alterations in the oxido-reductive status of mouse
red blood cells. Phytother Res 2000;14:215-217.
40. Badary, O. A., Abdel-Naim, A. B., Abdel-Wahab, M. H.&
Hamada, F. M. The influence of thymoquinone on doxorubicin-
induced hyperlipidemic nephropathy in rats.
Toxicology 2000;7:219-226.
41. Rangan, G. K., Wang, Y. & Harris, D. C. Induction of proteinuric
chronic glomerular disease in the rat (Rattus
Norvegicus) by intracardiac injection of doxorubicin
hydrochloride. Contemp Top Lab Anim Sci 2001;40:44-49.
42. Tesar, V., Zima, T., Jirsa, M., Crkovska J., Stipek S., Vernerova
Z.& Serakova M. Influence of losartan and enalapril on urinary
excretion of 8-isoprostane in experimental nephrotic
syndrome. Med Sci Monit 2002;8:23-28.
43. Bertolatus, J. A., Klinzman, D., Bronsema, D. A., Ridnour, L.
& Oberley, L. W. Evaluation of the role of reactive oxygen
species in doxorubicin hydrochloride nephrosis. J Lab Clin
Med 1991;118:435-445.

Thank you for copying data from http://www.arastirmax.com