You are here

DENEYSEL SICAK RENAL ÎSKEMÎ VE REPERFÜZYONDA LÎPÎD PEROKSÎDASYONU VE ALPHA-TOCOPHEROL TEDAVİSİNİN DEĞERLENDİRİLMESİ

THE EVALUATION OF LIPID PEROXIDATION AND ALPHA-TOCOPHEROL TREATMENT IN EXPERIMENTAL WARM RENAL ISCHEMIA AND REPERFUSION

Journal Name:

Publication Year:

Keywords (Original Language):

Abstract (2. Language): 
We investigated effects of alpha-tocopherol on li-pid peroxidation and histological observations indu¬ced by oxygen free radicals generated during warm renal ischemia and reperfusion in 50 Guinea Pigs. Twenty-three Guinea Pigs were divided to five groups. Group 1 (n:10) was healthy controls. Group 2 (n:10) had warm renal ischemia for 30 minutes. Group 3 (n:10) was tretad intraperitoneal^ by alp-ha-tocopherol (100 mgper day) for three days before warm renal ischemia. Group 4 (n:10) had reperfusi-on for 60 minutes after warm renal ischemia for 30 minutes. Group 5 (n:10) was treated intraperitone-ally by alpha-tocopherol before warm renal ischemia and reperfusion. In Group 4, level of plasma malondialdehyde (me¬dian value: 9.52 nmollmL), an indicator of lipid pe-roxidation, was higher than those of Group 1, 2, 3 and 5 (6.67, 6.59, 6.56, 657 nmollmL, respectively; p<0.001 for all groups). Histological observations showed necrosis of pro¬ximal and distal convoluted tubules following warm renal ischemia. This necrosis area expanded, and glomerulus injury started following reperfusion. The treatment of alpha-tocopherol did not affect to histo-logical changes of renal ischemia, but it reduced re-perfusion injury after renal ischemia. Finally, it was shown that alpha-tocopherol redu¬ced both lipid peroxidation and histologic injury in reperfusion following warm renal ischemia.
Abstract (Original Language): 
îskemi sonrası reperfüzyonla meydana gelen oksijen serbest radikalleri iskemik akut renal yetmezlik patogenezinde ve renal transplantasyon sonrası al-lograft fonksiyonunun gecikmesinde önemli rol oynar¬lar. Bu çalışmada 50 Guinea Pigde sıcak renal iske-mi ve reperfüzyon sırasında oluşan oksijen serbest radikalleriyle meydana gelen Lipid peroksidasyonu ve renal histolojik değişiklikler üzerinde alpha-tocophe-rol (E vitamini) tedavisinin etkilerini incelemeyi amaçladık. 50 Guinea Pig, 5 gruba bölündü. Grup 1 (n=10) sağlıktık kontrollerdi. Grup 2 (n=10)'ye 30 dk. sürey¬le sıcak renal iskemi uygulandı. Grup 3 (n-10)'e sı¬cak renal iskemi öncesi 3 gün süreyle intraperitoneal alpha-tocopherol (100 mg/gün) verildi. Grup 4 (n=10)'e 30 dk. sıcak renal iskemi sonrası 60 dk. re-perfüzyon yapıldı. Grup 5 (n-10)'e intraperitoneal alpha-tocopherol verildi ve sıcak renal iskemiyi taki¬ben reperfüzyon uygulandı. Grup 4'de lipid peroksidas)'onunun bir göstergesi olan plasma malondialdehit düzeyleri (median değer: 9.52 nmollmL) sırasıyla Grup 1,2,3 ve 5den daha yüksek bulundu (6.67, 6.59, 6.56, 6.57 nmollmL, p < 0.001 tüm gruplar için). Renal histolojik incelemede ise sıcak renal iskemi-yi takiben proksimal ve distal tubuluslarda nekroz gözlendi. Reperfüzyonu takiben bu nekroz sahası ge¬nişledi ve glomerulus hasarı başladı. Alpha-tocophe-rolle tedavi, renal iskeminin histolojik değişikliklerini etkilemedi, fakat renal iskemi sonrası reperfüzyon ha¬sarını azattı. Sonuç olarak, alpha-tocopherol'ün sıcak renal is-kemiyi takiben reperfüzyonda lipid peroksidasyonu ve renal histolojik hasarı azalttığı gösterildi.
FULL TEXT (PDF): 
5-10

REFERENCES

References: 

1. Diamond JR, Bonventre JV, Karnovsky ML A role for oxygen free radicals in aminonucleoside nephrosis. Kidney Int. 1986; 29: 478-483.
2. Bosco PJ, Shweizer RT. Use of oxygen radical sca¬vengers on autografted pig kidneys after warm ische¬mia and 48-hour perfusion preservation. Arch Surg
1988; 123: 601-604.
3. Miller WL, Thomas RA, Berne RM, et al. Adenosine production in the ischemic kidney. Circ Res 1978; 43: 390-397.
4. Waund WR, Rajagopalan KV. Purification and pro¬perties of the NAD+ - dependent (type D) and Q2-de-pendent (type O) forms of rat liver xanthine dehydro-genase. Arch Biochem Biophys 1976; 172: 354-64.
5. Fridovich I. Quantitative aspects of the production of superoxide anino radical by milk xanthine oxidase J Biol Chem 1970; 245: 4053A057.
6. Fridovich I. The biology of oxygen radicals. Science
1978; 201: 875-880.
7. Kellogg EW, Fridovich I. Superoxide, hydrogen pero¬xide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J Biol Chem 1975; 250: 8812-8817.
8. Kim K, Rhee SG, Stadman ER, Nonenzymatic cleava¬ge of proteins by reactive oxygen species generated by
dithiothreitol and iron. J Biol Chem 1985; 260: 394¬397.
9. Paller MS. Free radical-mediated post ischemic injury in renal transplantation. Ren Fail 1992; 14 (3): 257¬260. .
10. Jablonski P, Howden BO, Rae DA. An experimental model for assesment of renal recovery form warm isc-
hemica. Transplantation 1983; 35: 198-204.
11. Maessen JG, Van Der Vusse GJ, Vork M, et al. The benefici effect of intermediate normothermic perfusi-on during cold storage of ischemically injured kid¬neys. Transplantation 1989; 47(3): 409-414.
12. McDougal WC. Renal perfusion/Reperfusion injuries. J Urol. 1988; 140: 1325-1330.
13. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods in Enzymology, (Eds. Colowick SP and Kap¬lan NO), Vol. 52, Academic Press, New York, 1978,
pp: 302-310.
14. Galat JA, Robinson AV, Rhodes RS. Oxygen Free Ra¬dical Mediated Renal Dysfucniton. J Surg Res 1989;
46: 520-525.
15. Freeman BA, Crapo JD. Biology of disease: free radi¬cals and tissue injury. J Lab Invest 1982; 47: 412-426.
16. Marubayashi S, Dohi K, Sugino K, et al. The protecti¬ve effect of administered a-tocopherol against heptic damage caused by ischemia-reperfusion or endotoxe-mia. Ann NY Acad Sci 1989; 570: 208-218.
17. Demirbaş A, Bozoklu S, Özdemir A, et al. Effect of alpha-tocopherol on the prevention of reperfusion in¬jury caused by free oxygen radicals in the canine kid¬ney auto transplantation model, Transplant Proc 1993;
25(3): 2274.
18. Paller MS, Hoidal JR, Ferris TF. Oxygen free radicals in ishemic acute renal failure in the rat. J Clin Invest 1984; 74:1156-1164.
19. Dolşuoğlu HH, Aktan AO, Yeğen C, et al. The cytop-rotective effects of verapamil and iloprost (ZK 36374) on ischemia/reperfusion injury of kidneys. Transpl Int
1993; 6(3): 138-142.
20. Rabl H, Khoschsorur G, Colombo T, et al. A multivi-tamin infusion perevents lipid peroxidation and impro¬ves transplantation performance. Kidney Int 1993; 43: 912-917.
21. Frega NS, DiBona DR, Guetler B. et al. Ischemic re¬nal injury. Kidney Int. 1976; 10: 517-525.
22. Paller MS. Free radical-mediated post ischemic injury
renal transplantation. Ren Fail 1992; 14(3): 257-260.
23. Witting LA. Vitamin E and lipid antioxidants in free-radical initiated reactions. Free radicals in biology (Ed. WA. Pryor), Academic Press, New York, 1980;
. 295-319.
24. Diguiseppi J, Fridovich I. Ethylene from 2-keto -4-thi-omethyl butyric acid: The Haber-Weiss reaction. Arch Biochem Biophys 1980; 205: 323-329.
25. Demopoulos HB, Flamm ES, Hietronigro DD, et al. The free radical pathology and the microcirculation in the major central nervous system disorders. Acta PhysiolScand 1980: Suppl 492: 91-119.
26. Green CJ, Healing G, Simpkin S, et al. Allopurinol in¬hibits lipid peroxidation in warm ischameic and reper-fused rabbit kidneys. Free Radic Res Commun. 1989; 6(5): 329-337.
27. Marx A, Heberer M, Gale J, et al. Reduction of renal reperfusion damage following varm ischemia by allo-purinol and superoxide dismutase. Helv Chir Acta 1989; 56(4): 539-542.

Thank you for copying data from http://www.arastirmax.com