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The Effects of Vitamin E Application on Some Free Radicals and Lactate Levels in Acute Exercise

The Effects of Vitamin E Application on Some Free Radicals and Lactate Levels in Acute Exercise

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Abstract (2. Language): 
The aim of this study is to investigate the effect of vitamin E supplement on elite taekwondo performers who were made to do acute taekwondo exercise on some free radicals. Twenty-four healthy male elite taekwondo performers whose mean age is 21.66 ± 1.20 and mean body weight is 72.50 ±8.17 kg participated the study. The study protocol was also endorsed by the ethical board of the same faculty. In the study, the subjects were divided into 2 equal groups. The vitamin E group (Group 1) will be given vitamin E for 4 weeks (300 mg D-alpha- tocopheryl acetate) and will be made to do acute taekwondo exercise once a week till they get tired. The control group (Group 2) is the group who were only made to do acute taekwondo exercise till they got tired once a week for 4 weeks. Taekwondo exercises were carried out for four weeks and once a week as acute exercises till they got tired. During four weeks of application procedure, malondialdehyde (MDA) and Nitrococsite (NO) levels and plasma lactate (LAC) levels were determined from the blood samples taken from the subjects before the beginning of and at the end of the application and before and after the training. While the highest MDA level was obtained from the 2nd group in the 4th measurement (P<0.05), the lowest MDA level was obtained from 3rd measurement in the 1st group (P<0.05). While the highest NO level was obtained in the 2nd measurement in the 2nd group (P<0.05), the lowest NO level was obtained on the 3rd measurement in the 1st group (P<0.05). While the highest lactate level was obtained in the 2nd measurement in group 1 (P<0.05), the lowest lactate level was in the 3rd measurement in group 1 (P<0.05). As a result, it was determined that vitamin E application prevented free radical formation by increasing antioxidant activity during acute taekwondo exercise and substantially pressurized lactate levels.



Alessio HM. Exercise-induced oxidative stress. Med Sci
Sports Exercise, 1993; 25, 218.
2. Aquilo A, Tauler P, sureda A, Cases N, Tur J, Pons A.
Antioxidant diet supplementation enchances aerobic
performance in amateur sportsmen. J Sports Sci,
3. Aslan R. Sedanterlerde Akut ve Programlı Submaksimal
Egzersizin Eritrosit Membram Lipit Peroksidasyonu ve
Antioksidan Savunma Sistemi Üzerine Etkilerinin
Araştırılması, Yüzüncü Yıl Üniversitesi Sağlık Bilimleri
Enstitüsü, Fizyoloji Anabilimdalı, Yayınlanmamış
Doktora Tezi, Van, 1997.
4. Chae Ch, Shin CH, Kim HT. The combination of
alpha-lipoic acid supplementation and aerobic exercise
inhibits lipid peroxidation in rat skeletal muscles. Nutr
Res, 2008; 28(6): 399-405.
5. Child RB, Wilkinson DM, Fallowfield JL, Donnelly AE.
Elevated serum antioxidant capacity and plasma
malondialdehyde concentration in response to a
simulated half-marathon run. Med Sci Sports Exerc, 1998;
30: 1603–7.
6. Claro LM, Leonart MSS, Comar SR, Nascimento AJ.
Effect of vitamins C and E on oxidative processes in
human erythrocytes. Cell Biochem Funct, 2005; 24:531-
7. Davison GW, Hughes CM, Bell RA. Exercise and
mononuclear cell DNA damage: the effects of
antioxidant supplementation. Int J Sport Nutr Exerc
Metab, 2005; 15: 480-92.
8. Düzova H, Emre MH, Karakoç Y, Karabulut AB,
Yılmaz Z, Gürsul C, Yoloğlu S. Orta ve Yüksek
Düzeyde Treadmill egzersizinin Sıçanların Kas ve
Eritrosit Oksidan/Antioksidan Sistemine Etkisi. İnönü
Üniversitesi Tıp Fakültesi Dergisi. 2006;13:(1)1
9. Ferreira LF, Lutjemeier BJ, Townsend DK, Barstow TJ.
Effects of pedal frequency on muscle microvascular O2
extraction. Eur J Appl Physiol, 2005; 21: 1-6
10. Goldfarb AH. Antioxidants role of supplementation to
prevent exercise-induced oxidative stress. Med Sci Sports
Exercise, 1993; 25. 232.
11. Goldfarb AH, McIntosh MK, Boyer BT. Vitamin E
attenuates myocardial oxidative stress induced by
DHEA in rested and exercised rats. J Appl Physiol, 1996;
80: 486-90.
12. Goto C, Hıgashı Y, Kimura M, Noma K, Hara K,
Nakagawak, KM, Chayama K, Yoshizumi M, Nara I.
Effect of different intensities of exercise on
endothelium-dependent vasodilatation in humans: role
of endothelium-dependent nitric oxide and oxidative
stress. Circulation, 2003; 5,108(5):530-5. Epub.
13. Grant S, McMillan K, Newell J, Wood L, Keatley S,
Simpson D. Et al. Reproducibility of the blood lactate
threshold, 4 mmol.l (–1) marker, heart rate and ratings
of perceived exertion during incremental treadmill
exercise in humans. Eur J Appl Physiol, 2002; 87: 159–
14. Güllü E. Sedanterlerde ve Dayanıklılık Sporcularında
Maximal ve Submaximal Egzersiz Sonrası Oluşan
Oksidan Stres ve Antioksidan Düzeylerinin
Karşılaştırılması. Ankara, Doktora tezi.2007
15. Jenkins RR, Goldfarb A. Introduction oxidant stress,
aging, and exercise. Med Sci Spans Exercise, 1993; 25,210.
16. Joannidis M, Gstraunthaler G, Pfaller W. Xanthine
oxidase: evidence against a causative role in renal
reperfusion injury. Am J Physiol Renal Physiol, 1990; 258:
17. Viña J, Gomez-Cabrera MC, Lloret A, Marquez R,
Miñana JB, Pallardó FV, Sastre J. Free radicals in
exhaustive physical exercise: mechanism of production,
and protection by antioxidants. IUBMB Life, 2000; 50:
271- 277.
18. Kaczmarski M, Wójcicki J, Samochowiec L, Dutkiewicz
T, Sych Z. The influence of exogenous antioxidants and
physical exercise on some parameters associated with
production and removal of free radicals. Pharmazie,
1999; 54: 303-6.
19. Kanter M. Free radicals and exercise: Effects of
nutritional antioxidant supplementation. Exerc Sport Sci
Rev, 1995; 23: 375-398.
20. Kanter M. Free radicals, exercise and antioxidant
supplementation. Proc Nutr Soc, 1998; 57:9-13
21. Kim HT. Effect of the joint administration of selenium
and vitamin E in combination with regular aerobic
exercise on markers of lipid peroxidation and
glutathione peroxidase in diabetic rats. Int J Sport Nutr
Exerc Metab, 2005; 15: 266-78.
22. Koca F, Süer C, Erol E. The ergogenic effect of the
sodium bicarbonate on short term high intensity.
Anaerobic exercises in which are at different altitude.
Erciyes University Journal of Health Sciences, 2004; 13(2) 39-
23. Machefer G, Groussard C, Vincent S, Zouhal H, Faure
H, Cillard J, Radák Z, Gratas-Delamarche A.
Multivitamin-mineral supplementation prevents lipid
peroxidation during "the Marathon des Sables. J Am
Coll Nutr, 2007; 26(2): 111-20.
24. Minamiyama Y, Takemura S, Suehiro S, Okada S.
Vitamin E deficiency accelerates nitrate tolerance vi a
decrease in cardiac P 450 expression and increased
oxidative stress. Free Radic Biol Med, 2006; 1;40 (5): 808-
5. Novelli GP, Bracotti G, Falsini S. Spin-trappeers and
Vitamin E prolong endurance to muscle fatigue in mice.
Free Radic. Biol. Med. 1990; 8:9-13.
26. Reddy KV, Kumar TC, Prasad M, Reddana P.
Pulmonary lipid peroxidation and antioxidant defenses
during exhaustive physical exercise: the role of vitamin
E and selenium. Nutrition, 1998; 14: 448–451.
27. Rodas G, Ventura JL, Cadefau JA, Cusso R, Parra J. A
short training programme for the rapid improvement of
both aerobic and anaerobic metabolism. Eur J Appl
Physiol. 2000; 82: 480–486.
28. Sahlin K, Ekberg K, Cizinsky S. Changes in plasma
hypoxanthine and free radical markers during exercise
in man. Physiol Scand, 1991; 145: 275-281.
29. Saito M, Miyagawa I. Real-time monitoring of nitric
oxide in ischemia-reperfusion rat kidney. Urol Res, 2000;
30. Schulpis KH, Moukas M, Parthimos T, Tsakiris T,
Parthimos N, Tsakiris S. The effect of alpha
Tocopherol supplementation on training-induced
elevation of S100B protein in sera of basketball players.
Clin Biochem, 2007; 40(12):900-6.
31. Simon-Schnass , Pabst H. Influence of Vitamin E on
physical performance. Int J Vit Nutr Res, 1988; 58:49-
32. Tiidus PM, Houston M E. Vitamin E status and
response to exercise training. Sports Med, 1995; 20:12-23.
33. Traverse JH, Wank YL, Ruisheng D, Nelson D,
Lındstorm P, Archer LS, Gong G, Bache JR. Coronary
NO production in response to exercise and entothelium
dependent agonist. Circulation 101: 2526- 2531,2000
34. Tsakiris S, Karikas GA, Parthimos T, Tsakiris T,
Bakogiannis C, Schulpis KH. Alpha_tocopherol
supplementation prevents the exercise-induced
reduction of serum paraoxonase 1/arylesterase activities
in healty individuals. Eur J Clin Nutr, 2009; 63(2):215-21
35. Ushiyama M, Kuramochi T, Yagi S, Katayama S.
Antioxidant treatment with alpha-tocophrol improves
erctile funtion in hypertensive rats. Hypertens Res, 2008;
31 (5): 1007-13
36. Vollaard NB, Shearman JP, Cooper CE. Exerciseinduced oxidative stress: Myths, Realities and
Physiological Relevance. Sports Med, 2005; 35: 1045-62

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