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BAKTERİYEL GASTROENTERİTLERDE DIŞKIDA LAKTOFERRİN DÜZEYLERİ

STOOL LACTOFERRİN LEVELS IN BACTERIAL GASTROENTERITIS

Journal Name:

  • Türkiye Aile Hekimliği Dergisi

Publication Year:

  • 2000

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
Diarrheal illnesses are very common in communities and hospitals. In these cases, diagnostic approaches may be expensive and non cost-effective. Direct microscopic examination and stool culture are the methods commonly used for diagnosis of these cases. Detection of leukocytes in direct microscopic examination is very important in managing the treatment but in this method fresh stool samples must be used and the experience of the analyst is a major factor. Stool cultures require more complicated laboratory techniques. In this study, we explored stool lactoferrin levels to detect leukocytes as an inexpensive and simple method. For this purpose we exp¬lored anti-lactoferrin antibody coated latex particles agglutination in stool suspensions. In our study, we detected 93.75% (+), 68.75% (++) agglutination in specimens that were positive for leukocytes in direct microscopic examination. Sensitivity of our study was evalu¬ated to be 68.75%. In conclusion, we suggest that anti-lactoferrin antibody coated latex particle agglutination may be used to detect leukocytes in stool specimens.
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Abstract (Original Language): 
Enteritler, hastanelerde ve toplumda oldukça sık görülen hastalıklar olduğundan, bu olgularda tanısal yaklaşımlar önemli bir sorun oluşturmaktadır. Bu konuda en sık uygulanan yöntem direkt dışkı mik-roskopisinde lökosit saptanmasıdır. Bu yöntem dışkının kısa süre içinde incelenmesini gerektirir ve yöntemi uygulayan kişinin bu konudaki yetkinliği de önemli bir faktördür. Dışkı kültürleri için ise gelişmiş laboratuvar koşulları gerekir. Biz bu çalışmada dışkıda lökositler ile laktoferrin arasındaki ilişkiyi araştırdık. Bu amaçla anti-laktoferrin antikoru kaplı lateks partikül-leri kullanarak dışkı süspansiyonlarında aglütinasyon arandı; lökosit belirlenen olguların %93.75'inde (+) ve %68.75'inde (++) olarak saptandı. Dışkı kültürlerinden sadece bir tanesinde üreme olması nedeniyle anlamlı bir karşılaştırma yapılamadı. Tüm olgular ele alındığında toplam %49.9 pozitif sonuç olması anlamlı olmadığından la-boratuvarımızda hazırlanan solüsyonla (++) olarak bulunan değerler temel alınarak çalışmamızın sensitivitesi %68.75 olarak değerlendirildi. Sonuç olarak antikor kaplı lateks partikülleriyle aglütinasyon saptanmasının dışkıda lökosit aranması için kullanılmasını öneriy¬oruz.
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  • 1-4
19-23
  • Turkish

REFERENCES

References: 

1. Koplan JP, Fineberg HV, Ferraro MJB, Rosenberg ML. Value of stool cultures. Lancet 1980; 28: 1031-5.
2. Guerrant RL, Shields LDS, Thorson SM, Schorling JB, Groschel
HM. Evaluation and diagnosis of acute infectious diarrhea. Am J Med
1985; 78: 91-2.
3. Guerrant J, Raudin J, Brush R, Sullivan J, Mander JL. Interaction between Entameoba hystolitica and human polimorphonuclear neutrophils. J Infect Dis 1981; 143: 83-93.
4. Vogtlin J, Stalder H, Hurzler L. Modified guaiac test may replace search for faecal leukocytes in acute infectious diarrhea. Lancet 1983; 2:
1204.
5. Huicho L, Sanchez D, Contreras M. Occult blood and fecal leukocytes as screening tests in childhood infectious diarrhea: an old problem revisi¬ted. Pediatr Infect Dis J 1993; 12: 474-7.
6. Choi SW, Park CH, Soiva TMJ, Zaenker EI, Guerrant RL. To culture or not to culture: Fecal lactoferrin screening for inflammatory bacterial diarrhea. J Clin Microbiol 1996; 34(4): 928-32.
7. Guerrant RL, Araujo V, Soares E ve ark. Measurement of fecal lacto-ferrin as a marker of fecal leukocytes. J Clin Microbiol 1992; 30(5):
1238-42.
8. Guerrant RL, Martins C, Silva J. Fecal lactoferrin as a marker of le-ukcytes (letter). J Clin Microbiol 1994; 32(10): 2629-30.
9. Schleupner MA, Garner DC, Sosnowski KM, ve ark. Concurrence of Clostridium difficile toxin A enzyme linked immunosorbent assay and clinical criteria with C. difficile cytotoxin titer in two patient cohorts. J
Clin Microbiol 1995; 33(7): 1755-9.
10. Oneson R, Groschel DH. Leukocyte esterase activity and nitrit tests as a rapid screen for significient bacteriuria. Am J Clin Pathol 1985; 83: 84-7.
11. Hetherington SV, Spitzngel JK, Quie PG. An enzyme linked immunoassay (ELISA) for measurement of lactoferrin. J Immunol Methods 1983;
65: 183-90.
12. Pryzwansky KB, Martin LE, Spitznagel JK. Immunohistochemical localization of myeloperoxidase, lactoferrin, lysosime and neutral proteases in human monocytes and neutrophilic granulocytes. J Reticuloendothel Soc 1978; 24: 295-309.
13. Yong WH, Mattia AR, Fuerraro MJ. Comparison of fecal lactoferrin la¬
tex agglutination assay and methylen blue microscopy for detection of fe¬cal leukocytes in Clostridium difficile-associated disease. J Clin Microbi-
ol 1994; 32(5): 1360-1.
14. Huicho L, Campos M, River J, Guerrant RL. Fecal screening tests in the approach to acute infectious diarrhea: a scientific overview. Pediatr
Infect Dis J1996; 15(6): 486-94.
15. Speelman P, McGlaughlin R, Kabir T, Btuler T. Differential clinical features and stool findings in shigellosis and ameobic dysentry. Trans R Soc Trop Med Hyg 1987; 81: 549-51.
16. Tenovuo J. Nonimmunoglobulin defense factor in human saliva. In: J. Tenovuo (ed.), Human saliva; Clinical chemistry and microbiology.
17. Cohen MS, Britigan BE, French M, Bean K. Preliminary observations on lactoferrin secretion in human vaginal mucus: variations during menst-ruel cycle, evidence of hormonal regulation and implications for infecti¬on with Neisseria gonorhoeae. Am J Obstet Gynecol 1987; 157: 1122-5.
18. Andreoli TE, Bennett JC, Carpenter CJ, Plum F, Smith LH. Cecil Es¬sentials of Medicine, 3. Ed., WB Saunders Company, Philadelphia, 1993. Lacey SW, Richter JE, Wilcox CM, Sindirim Hastalıkları, Tuzcu M. (Çev. Ed.), Türkçe 1. baskı. İstanbul, Yüce Yayınları, 1995, 71-7.

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