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Süt, Et, İdrar ve Yemde Altı Farklı Grup Antimikrobiyal İlacın Biochip Array-Based Immunoassay ile Aynı Anda Tespiti

Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices

Journal Name:

  • Uludag University Journal of The Faculty of Veterinary Medicine

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Antimicrobials are widely used for animal health in food-producing animals. Therefore, antimicrobial residues are of food safety concern. Simultaneous detection of different antimicrobial residues in the same ma-trice is important. Therefore, the objective of this study was to detect six different group antimicrobials in milk, meat, urine, and feed matrices under experimental conditions, and to evaluate its use for routine analysis. Bio-chip array-based immunoassay is currently used for simultaneous detection and quantitation of different groups of six antimicrobials in milk, urine, meat, honey, and feed. Results showed that of the six target drugs, norfloxa-cin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in milk, meat, urine, and in feed matrices. In conclusion, this assay can effectively detect all target antibacterials from different groups in milk, meat, urine, and feed matrices, and this assay can be used for routine detection of these antibacterial resi-dues in the stated matrices.
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Abstract (Original Language): 
Antimikrobiyaller besin üretiminde faydalandığımız hayvanlarda yaygın olarak kullanılmaktadır. Bu ne-denle, bu besinlerde bulunabilen antimikrobiyal kalıntıları insan sağlığı açısından önemlidir. Farklı antimikrobi-yal kalıntılarının aynı numunede ve aynı anda birlikte tespiti önemlidir. Bu nedenle, bu çalışmanın amacı Bioc-hip array-based immunoassay yöntemi ile altı farklı grup antibakteriyel ilacın süt, et, idrar ve yemde deneysel şartlarda tespit etmektir. Bu test, aynı anda altı farklı grup antimikrobiyalin süt, idrar, et, bal ve yem numunele-rinde miktarlarının belirlenmesinde kullanılmaktadır. Bu metodla hedef ilaçlar olan norfloksasin, seftifor, florfe-nikol, streptomisin, taylosin ve tetrasiklin süt, et, idrar ve yemde tespit edildi. Sonuç olarak, bu test ile yukarıda belirtilen altı farklı gruptaki hedef antibakteriyel ilaç süt, et, idrar ve yemde etkili bir şekilde tespit edilebilmek-tedir. Bu metod, belirtilen numunelerde bu antibakteriyel ilaçların kalıntılarının rutin analizinde kullanılabilir.
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  • Turkish

REFERENCES

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