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Polimeraz Zincir Reaksiyonu (PCR) Optimizasyonu

Optimization of Polymerase Chain Reaction (PCR)

Journal Name:

  • Uludag University Journal of The Faculty of Veterinary Medicine

Publication Year:

  • 2013

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Polymerase chain reaction (PCR), a deoxyribonucleic acid (DNA) that lies between two known chain enzymatically amplify a specific DNA region as an in vitro technique becoming common everyday. PCR meth-od, used for many purposes such as diagnosis, epidemiology and studies to determine the amount of DNA, are still under development. Microbiology is taking a significant proportion in PCR usage, like innovations of appli-cation in other fields. Furthermore, PCR is the fundamental molecular method that being used today for many purposes in detection of human and animal pathogenic microorganisms. It is needed to optimization of PCR parameters of each gene, regardless even what was targeted. PCR may be required to perform it again, even between different instruments and laboratories. In this review, PCR application in microbiology, the preperation of main standards must be followed at all stages of PCR reagents and materials when PCR a designed, will be mentioned.
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Abstract (Original Language): 
Polimeraz zincir reaksiyonu (PCR), bir deoksiribo nükleik asit (DNA) zincirinin bilinen iki parçası arasın-da uzanan özel bir DNA bölümünün enzimatik olarak çoğaltıldığı in vitro bir teknik olarak her geçen gün yay-gınlaşmaktadır. PCR metodu, teşhis, epidemiyolojik ve DNA miktarı belirleme çalışmaları gibi birçok amaçla kullanılmakta ve geliştirilmeye devam edilmektedir. PCR’ın kullanıldığı tüm alanlarda meydana gelen gelişme-lerden, mikrobiyoloji alanı da önemli oranda payını alarak gelişmeye devam etmektedir. İnsan ve hayvan kay-naklı patojenik mikroorganizmalar için pek çok amaçla kullanılan PCR aynı zamanda günümüzde kullanılan birçok moleküler metodun temelini oluşturmaktadır. Reaksiyon her ne amaçla çalışılırsa çalışılsın, her gen böl-gesi için, kullanılacak reagent ve PCR parametrelerinin optimizasyonunun yapılması gerekmektedir. Hatta yapı-lan optimizasyonun, PCR’ın gerçekleştirileceği farklı aletler ve laboratuvarlar arasında bile tekrar yapılması gerekebilmektedir. Bu derlemede, kısaca PCR’ın mikrobiyolojide kullanımı, PCR dizayn edilirken ve PCR’ın tüm aşamalarında kullanılan reagentler ve malzemelerde uyulması gereken ana standartlardan bahsedilecektir.
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