You are here

Home » Content

Standardization of PCR conditions for an Ancient DNA Amplification

Journal Name:

  • Uluslararası İnsan Bilimleri Dergisi

Publication Year:

  • 2012

Key Words:

Author NameUniversity of Author
Abstract (2. Language): 
An ancient DNA provides us a powerful tool to study the miniscule amounts of DNA present in hundreds of thousands of years old archaeological remains. Since the advent of the PCR, it became possible for the population biologists to use this scarce and rare genetic material (aDNA) to understand prehistoric population histories. Working with ancient DNA is challenging in itself as it needs a manifold attention in order to maintain the archaeological sample free from contemporary DNA contamination. Apart from that, there are several other complications associated with ancient DNA work such as the preservation of DNA itself that is in degraded state and low copy number, DNA isolation and its successful PCR amplification. Despite the critical role of PCR in this field of research, till date no study has comprehensively evaluated ancient DNA amplification. In this paper, we have reported our results to optimize PCR component as well as PCR condition to amplify HVR1 region in 600 years old biological samples
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon arastrmx_161047_9_pp_102-109.pdf
  • 1
102-109
  • English

REFERENCES

References: 

Bouwman, A.S. and T.A. Brown. 2002. Comparison between silica-based methods for the
extraction of DNA from human bones from 18th to mid-19th century London. Anc.
Biomol. 4:173-178.
Cappellini E, Chiarelli B, Sineo L, Casoli A, 2004, Journal of Archaeological Science,
Biomolecular study of the human remains from tomb 5859 in the Etuscan necropolis of
Monterozzi, Tarquinia (Viterbo,Italy), 31, 603-612.
Hanni, C., T. Brousseau, V. Laudet, and D. Stehelin. 1995. Isopropanol precipitation
removes PCR inhibitors from ancient bone extracts. Nucleic Acids Res. 23:881-882.
Hebsgaard M B, Phillips M J. and Willerslev E, 2005, Geologically ancient DNA: fact or
artefact? Trends in Microbiology, 13, 212-220.
Higuchi R, Bowman B, Freiberger M, Ryder OA, Wilson AC (1984). DNA sequences
from the quagga, an extinct member of the horse family. Nature 312 (5991): 282–4.
Hofreiter, M., G. Rabeder, V. Jaenicke-Despres, G. Withalm, D. Nagel, M. Paunovic, G.
Jambresic, and S. Pääbo. 2004. Evidence for reproductive isolation between cave bear
populations. Curr. Biol.14:40-43.
Hoss, M. and S. Pääbo. 1993. DNA extraction from Pleistocene bones by a silica-based
purification method. Nucleic Acids Res. 21:3913-3914.
Hummel, S. 2003.In Ancient DNA Typing. Springer Verlag, Berlin. aDNA extraction, p.
57- 63
Kaestle. F.A and Horsburgh.K.A, Am J Phys Anthropology, 2002, Suppl, Ancient DNA
in Anthropology: methods, applications, and ethics, 35, 92-130.
Kalmar T, Bachrati C Z, Marcsik A and Rasko I, 2000, A simple and efficient method for
PCR amplifiable DNA extraction from ancient bones, Nucleic Acids Research, 28, e67.
Kalmar, T., C.Z. Bachrati, A. Marcsik, and I. Rasko. 2000. A simple and efficient method
for PCR amplifiable DNA extraction from ancient bones. Nucleic Acids Res. 28:E67.
Kemp B M and Smith D G, 2005, Use of bleach to eliminate contaminating DNA from
the surface of bones and teeth, Forensic Science international, 154, 53-61.
Leonard, J.A., R.K. Wayne, and A. Cooper. 2000. Population genetics of ice age brown
bears. Proc. Natl. Acad. Sci. USA 97:1651- 1654.
Paabo, S., J.A. Gifford, and A.C. Wilson. 1988. Mitochondrial DNA sequences from a
7000-year old brain. Nucleic Acids Res 16:9775-9787.
Paabo,S., Poinar, H., Serre, D., Jaenicke-Després, V., Hebler, J., Rohland, N., Kuch, M.,
Krause, J., Vigilant, L., and Hofreiter, (2004) M. Ann. Rev. Genetics 38: 645-79.
Prado, M., C.M. Franco, C.A. Fente, A. Cepeda, B.I. Vazquez, and J. Barros- Velazquez.
2002. Comparison of extraction methods for the recovery, amplification and speciesspecific
analysis of DNA from bone and bone meals. Electrophoresis 23:1005-1012.
Shinoda K, Adachi N, Guillen S and Shimada I, 2006,Mitochondrial DNA Analysis of
ancient Peruvian Highlanders, Am J Phys Anthropology, Sep, 131, 98-107.
Thomas M, Gilbert P, Cuccui J, White W, Lynnerup N, Titball R W, Cooper A and
Prentice M B., 2004, Absence of yersinia pestis-specific DNA in human teeth from five
European excavations of putative plague victims, Microbiology, 150, 341-354.
Willerslev E and Cooper A, Ancient DNA, 2005, Pro.R.Soc.B, 272, 3-16.
Willerslev E, Hansen A J. and Poinar H N, 2004,Isolation of nucleic acids and cultures
from fossil ice and permafrost, TRENDS in Ecology and Evolution, 19, 141-147.
Yang, D.Y., B. Eng, J.S. Waye, J.C. Dudar, and S.R. Saunders. 1998. Technical note:
improved DNA extraction from ancient bones using silica-based spin columns. Am. J.
Phys. Anthropol. 105:539-543.

Thank you for copying data from http://www.arastirmax.com