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Cape'nin Süperoksit Süpürücü Kapasitesinin In Vitro incelenmesi

Investigation of In Vitro Superoxide Scavenging Capacity of Cape

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2002

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Keywords (Original Language):

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Abstract (2. Language): 
introduction: The antioxidant capacity of CAPE (caffeic acid phenethyl ester) has been investigated in recent years but, so far most of the studies were in vivo studies. in this study we investigated, the in vitro antioxidant capacity of CAPE to scavenge superoxide radicals. CAPE, an active component of propolis from honeybee hives, is known to have anti mitogenic, antioxidant, anticarcinogenic, antiinflammatory and immunomodulatory properties. Aim of the study: CAPE is an antioxidant molecule in vivo. The aim of this study is to investigate if CAPE under in vitro conditions has the same effect similar to in vivo. Methods: The method developed by McCord and Fridovich for the determination of SOD activity is modified by replacing SOD with CAPE. Results: We find that, CAPE has superoxide scavenging capacity in a dose dependent manner. The presence of 50 iimol/L CAPE caused 73.66 % percentage of inhibition in cyt c reduction.
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Abstract (Original Language): 
Giris:Son yillarda CAPE (Kafeik asi t fenil esteri)'nin antioksidan kapasitesi arastirilmistir, ancak su ana kadar yapilmis olan çalismalarin çogu in vivo çalismalardir. Biz bu çalismada CAPE'nin süperoksit radikallerine karsi antioksidan kapasitesini in vitro arastirdik. CAPE bal arisi kovani propolisinin aktif bir bileseni olup antimitojenik, antioksidan, antikarsinojen, antiinflamator ve immünomodülatör özellikleri bilinmektedir. Amaç: CAPE in vivo antioksidan bir moleküldür. Bu çalismada, CAPE'nin benzer kosullarda ancak in vitro ortamda ayni etkiyi gösterip göstermediginin arastirilmasi amaçlandi. Metod: McCord ve Fridovich'in SOD aktivitesi tayini için gelistirdikleri yöntem, SOD yerine CAPE kullanilarak modifiye edildi. Bulgular: CAPE'nin doza bagimli olarak artan bir süperoksit süpürücü kapasiteye sahip oldugunu saptadik. 50 ? mol/L CAPE varliginda cyt c redüksiyonu %73,66 inhibe oldu.
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