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Asthenozoospermia olgularında sperm morfolojisi değerlendirmede Spermac ve Diff-quik boya yöntemlerinin karşılaştırılması

Comparison of Spermac and Diff-quik staining methods in the assessment of sperm morphology in asthenozoopermia cases

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author Name
Abstract (2. Language): 
Sperm motility problems (Asthenozoopermia) is one of the major causes of male infertility. In cases with asthenozoopermia infertility increases to a greater extent when sperm morphology disorders are added to coexisting motility disorders. A total of 65 males (21 males with sperm motility disorder and 44 males without this disorder) who referred to the in vitro fertilization unit of Dokuz Eylul University Medical School between November 15, 2004 and April 5, 2005 were included in the present study. Spermac and Diff-quik staining methods were used in the assessment of sperm morphology in all semen samples. Test results showed consistency with both staining methods on 55 semen samples examined. Sperm morphology was normal in 32 semen samples with consistent test results by both methods, while the percentage of morphology was low in 23 cases. In 7 of remaining 10 cases, the sperm morphology was in the normal range with Diff-quik staining method while an abnormal percentage was detected with Spermac method. In 3 cases the sperm morphology was within normal limits with Spermac staining method while Diff-quik staining method demonstrated abnormal sperm morphology. Statistical analysis with Mc Nemar test demonstrated that both staining methods were reliable. Since neither of the two methods used in the evaluation of sperm morphology has proven superiority to the other, a cost reduction may be provided by choosing the economical one.
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Abstract (Original Language): 
Sperm hareket bozuklukları (asthenozoopermia) erkek infertilitesi nedenleri arasında önemli bir yer tutmaktadır. Asthenozoopermia olgularında hareket bozukluğuna sperm morfolojisi bozuklukları eklendiğinde infertilitenin daha da arttığı saptanmıştır. Bu çalışmada 15 Kasım 2004 ile 5 Nisan 2005 tarihleri arasında Dokuz Eylül Üniversitesi Tıp Fakültesi Tüp Bebek Merkezi'ne başvuran toplam 65 erkek (sperm motilite bozukluğu olan 21 erkek ile motilite bozukluğu olmayan 44 erkek) çalışma kapsamına alındı. Tüm semen örneklerine sperm morfolojisi bakısında Spermac ve Diff-quik boya yöntemleri uygulandı. İncelenen 55 semen örneğinde test sonuçları her iki boyada da uyumluluk gösterdi. Test sonuçları uyumlu olan 32 semen örneğinde sperm morfolojisi her iki testte de normal bulunurken, 23 olguda morfoloji yüzdesi düşük bulundu. Geri kalan 10 olgunun ise 7'sinde sperm morfolojileri Diff-quik boya ile normal yüzdede bulunurken, Spermac boya ile anormal bir yüzde saptandı. Üç örnekte ise Spermac boya ile sperm morfolojisi normal sınırlarda gözlenirken, Diff-quik boya ile anormal sperm morfolojisine rastlandı. Yapılan Mc Nemar istatistiksel değerlendirmede her iki boya yönteminin de güvenilir olduğu saptandı. Sperm morfolojisi bakısı için kullanılan iki testin birbirine olan üstünlüğünün saptanmaması nedeniyle, bu testlerden ekonomik olanının seçilerek maliyetin düşürülmesi sağlanabilir.
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  • 1
23-26
  • Turkish

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