Buradasınız

Kadavra Tespitinde Kullanılan Formaldehitin Sıçan Karaciğerinde Oluşturduğu Hasar ve Buna Omega-3 Yağ Asitlerinin Etkisi

Damage in Rat Liver by Administration of Formaldehyde that Using Fixation of Cadaver and the Effects of Omega-3 Fatty Acids on This Damage

Journal Name:

Publication Year:

Keywords (Original Language):

Abstract (2. Language): 
Objectives: The toxic effects of formaldehyde on liver and protective effects of omega-3 fatty acids against these toxic effects were investigated. Materials and Methods: 21 adult male Wistar rats were divided into three groups. Rats in group I were used as control. Rats in group II were injected with formaldehyde every other day. Rats in group III daily received omega-3 fatty acids with injection of formaldehyde. At the end of 14-days experimental period, all rats were killed by decapitation. The liver tissue specimens which were taken from the rats were examined in light microscope with passing on the routine histological procedures. The activites of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), xanthine oxidase (XO) and malondi aldehyde (MDA) were determined in the liver specimens by using spectrophotometric methods. Results: In rats whom formaldehyde was given a statistically significant increase in the levels of SOD, CAT, GSH-Px, XO and MDA was determined when compared to control group. In the light microscopic examination of mis group, mononuchlear cell infiltration on portal area and araund central ven was determinated. There were hiperchromatic nucleus and stoplasmic vacuolization in hepatocytes. It was seen mat the histological variances which caused by formaldehyde exposure in rats were decreased whom received omega-3 fatty acids. A decrease of SOD, CAT, GSH-Px, XO, MDA levels were determined. Conclusion: It was determined that damage occured in liver tissue of rats exposured to formaldehyde and mis damage was reduced by omega-3 fatty acids.©2005, Fırat Üniversitesi, Tıp Fakültesi
Abstract (Original Language): 
Amaç: Formaldehitin karaciğer dokusu üzerine olan toksik etkileri ve bu toksik etkilere karşı omega-3 yağ asitlerinin koruyucu etkisi araştırıldı. Gereç ve Yöntem: 21 adet Wistar-Albino cinsi erkek sıçan üç gruba ayrıldı. Grup I'deki sıçanlar kontrol olarak kullanıldı. Grup II'deki sıçanlara gün aşırı olarak formaldehit enjekte edildi. Grup III'deki sıçanlara ise, formaldehit enjeksiyonu ile birlikte günlük olarak omega-3 yağ asiti verildi. 14 günlük deney süresi sonunda bütün sıçanlar dekapite edildi. Hayvanlardan alınan karaciğer doku örnekleri rutin histolojik prosedürlerden geçirilerek ışık mikroskobunda incelendi. Ayrıca doku örneklerinde glutatyon peroksidaz (GSH-Px), süperoksit dismutaz (SOD), katalaz (CAT), malondialdehit (MDA) ve ksantin oksidaz (XO) enzim aktiviteleri spektrofotometrik olarak tayin edildi. Bulgular: Formaldehit uygulanan sıçanlarda GSH-Px, SOD, CAT, XO ve MDA düzeylerinde kontrol grubuna göre istatistiksel olarak anlamlı bir artışın olduğu tespit edildi. Bu grubun ışık mikroskopik incelemesinde, portal alan ve vena centralis etrafında mononükleer hücre infİltrasyonu ayırt edildi. Ayrıca genişlemiş sinuzoidlerin kanla dolu olduğu gözlendi. Hepatositlerin hiperkromatik çekirdekli oldukları ve sitoplazmalarında vakuolizasyon olduğu tespit edildi. Formaldehit maruziyeti ile birlikte omega-3 yağ asiti verilen sıçanlarda ise, formaldehit maruziyetinin neden olduğu histolojik değişikliklerin azaldığı görüldü. Ayrıca SOD, CAT, GSH-Px, XO ve MDA düzeyi erininde azaldığı tespit edildi. Sonuç: Sıçanlarda formaldehit maruziyeti sonucu karaciğer dokusunda oksidatif hasarın oluştuğu ve bu hasarın omega-3 yağ asitleri uygulaması ile azaldığı tespit edildi. ©2005, Fırat Üniversitesi, Tıp Fakültesi
103-107

REFERENCES

References: 

1. Bolt HM. Experimental toxicology of formaldehyde. J Cancer Res Clin Oncol 1987; 113: 305-09.
2. Heck H, Casanova M. Pharmacodynamics of formaldehyde: Applications of a model for the arrest of DNA replication by DNA-protein cross- links. Toxicol Appl Pharmacol 1999; 160: 86-100.
3. Usanmaz SE, Akarsu ES, Vural N. Neurotoxic effects of acute and subacute formaldehyde exposures in mice. Envir Toxicol Pharmacol 2002; 11: 93-100.
4. Khanzadeh FA, Vaquerano MU, Khanzadeh MA, Bisesi MS. Formaldehyde exposure, acute pulmoner response and exposure control options in a gross anatomy laboratory. Am J Indust Med 1994; 26: 61-8.
5. Smith AE. Formaldehyde. Occup Med 1992; 42: 83-8.
6. Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. Formaldehyde evaluation from endodontic materials. Oral Health 1998; 88: 37-9.
7. Sarnak MJ, Long J, King AT. Intravesicular formaldehyde instillation and renal complications. Clin nephrol 1999; 51: 122¬25.
8. Eells JT, Mc Martin KE, Black K, Vırayotha V, Tısdell RH, Tephly TR. Formaldehyde poisoning. Rapid metabolism to formic acid. JAMA 1981; 246: 1237-38.
9. Koivusalo M, Koivula T, Uotila L. Oxidation of formaldehyde by nicotinamid dependent dehydrogenases. Prog Clin Biol Res 1982; 114: 155-68.
10. Songur A, Sarsilmaz M, Soğut S et al. Hypothalamic superoxide dismutase, xanthine oxidase, nitric oxide, and malondialdehyde in rats fed with fish omega-3 fatty acids. Prog Neuropsychopharmacol Biol Psychiatry 2004; 28: 693-98.
11. Yilmaz HR, Songur A, Ozyurt B, Zararsiz I, Sarsilmaz M. The effects of n-3 polyunsaturated fatty acids by gavage on some metabolic enzymes of rat liver. Prostaglandins Leukot Essent Fatty Acids 2004; 71: 131-35.
12. Alexander JW. Immunonutrition: the role of omega-3 fatty acids. Nutrition 1998; 14: 627-33.
13. Masters C. Omega-3 fatty acids and the peroxisome. Mol Cell Biochem 1996; 165: 83-93.
14. Stone NJ. Fish comsumption, fish oil, lipids, and coronary heart disease. Am J Clin Nutr 1997; 65: 1083-86.
15. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
16. Aebi H. Catalase. In: Bergmeyer U (ed.). Methods of enzymatic analysis. New York and London: Academic Press, 1974: 673-77.
17. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterisation of erythrocyte glutathione peroxidase. JLab Clin Med 1967; 70: 158-69.
18. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hidroxynonenal. Methods Enzymol 1990; 186: 407-21.
19. Prajda N, Weber G. Malignant transformation-linked imbalance: decreased xanthine oxidase activity in hepatomas. FEBS Lett 1975;59:245-49.
20.
Zararsı
z İ, Kuş İ, Yılmaz HR, Pekmez H, Ögetürk M, Sarsılmaz M. Sıçan prefrontal korteksinde formaldehit maruziyetiyle oluşan oksidatif hasara karşı omega-3 yağ asitlerinin koruyucu etkisi. Fırat Tıp Dergisi 2004; 9: 35-9.
21. Beall JR, Ulsamer AG. Formaldehyde and hepatotoxicity. J Toxicol Environ Health 1984; 14: 1-21.
22.
Yarıkta
ş M, Döner F, Doğru H, Aynalı G, Yönden Z, Delibaş N. Baş boyun malign tümörlerinde malondialdehit düzeyleri ve antioksidan enzim aktiviteleri. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2003; 10: 65-7.
23.
Sarsılma
z M, Özen OA, Özyurt H. Subakut ve subkronik formaldehit inhalasyonundan sonra sıçanlarda karaciğer enzimatik antioksidan sistemin değerlendirilmesi. Van Tıp Dergisi 2000; 7: 84-9.
24. Farooqui MY, Upreti RK, Ahmed AE, Ansari GA. Influence of intraperitoneally administered formaldehyde on bile production and tissue glutathione levels in rats. Res Commun Chem Pathol Pharmacol 1986; 53: 233-36.
25. Skrzydlewska E. Decreased antioxidant status and increased lipid peroxidation in rats after methanol intoxication. Rocz Akad MedBialymst 1996;41:397-404.
26. Teng S, Beard K, Pourahmad J, Moridani M, Easson E, Poon R. The formaldehyde metabolic detoxification enzyme systems and molecular cytotoxic mechanism in isolated rat hepatocytes. Chem Biol Interact 2001; 130-132: 285-96.
27. Dobrzynska I, Skrzydlewska E, Kasacka I, Figaszewski Z. Protective effect of N-acetylcysteine on rat liver cell membrane during methanol intoxication. Pharm Pharmacol 2000; 52: 547¬52.
28. Watanabe A, Saito S, Tsuchida T, Higuchi K, Okita M. Low plasma levels of docosahexaenoic acid in patients with liver cirrhosis and its correction with a polyunsaturated fatty acid-enriched soft oil capsule. Nutrition 1999; 15: 284-88.

Thank you for copying data from http://www.arastirmax.com