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DENEYSEL DÎYABETÎK NEFROPATÎDE ENALAPRİL VE L-KARNİTİN ETKİLERİ

EFFECTS OF ENALAPRİL AND L-CARNITINE IN EXPERIMANTAL DIABETIC NEPHROPATHY

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 2002

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Hyperglisemia, increased angiotensin II ATİ reseptör activity, oksidativestress, decreasing intracellular ATP play an important rol in occurance diabetic nephropathy (DN). In our study, effects of angiotensin converting enzyme inhibitor (ACEi) as enalapril (EN), and L-Carnitine (LC) were investigated on DN in streptozotocin-induced diabetic rats. Except healthy, patient control (HK, PK) groups, 2.5mg/kg/day EN and 100 mg/kg/day LC were given in a water for six weeks. After 24 hrs urine collection, bloodwith-drownedby cardiacpunction then, rats were sucrified. Erythrocytes count (Er), hemoglobin (Hb), hematocrit (Hct) in blood; and glucose, HbAlc, urea, creatinine (pCr), uric acid (UA), P04, Ca2*, Na, K*, albumin (Alb) in plasma;superokside dismutase enzyme (SOD) as an antioxidant, malonyl dialdehyte (MDA) as lipidperoxidationproduct (ROS) were measured in eryhtrocyte were measured. Daily excretion of urinary glucose (UG), albumin (UAE), N-acetyl-b-D-glucosaminidase (NAG) were measured. FractionalNa*(FANA) excretion and GFR were calculated. İmmunuhistochemical kollagene type IV accumulation and renal histopathology were investigated on light microscopy. After STZ injection DN was established with increased glucose, HbAlc, UG, UAE, NAG (P<.000), urea, pKr (P<005). According to PKgroup, lower UG (P<000), NAG, urine volume, P04, and higher Alb (P<05) in EN group, higher Er, Hb, lower urine volume, UG, NAG (P<.05) in LC groups were found. According to HK, nonsignificant increased MDA in PK, unchanged in EN and LC groups were found. Tubulo-glomerular basal membrane thickness and accumulation tip IVkollagen as DN findings significant in PK, moderate EN, and mild degree in LC group were found. Ourfindings suggests that, LC have protective effect on diabetic nephropathy like ACEi.
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Abstract (Original Language): 
Diabetik nefropati (DN) oluşmasında hiperglisemi, anjiotensin II ATİ reseptör aktivasyon ve oksidatif stres artısı, hücre içi ATP azalması önemli rol alır. Çalışmamızda, streptozosin (STZ) ile diyabet oluşturulan rotlarda anjiotensin /converting enzim inhibitörü (ACEi) olan Enalapril (EN) ve L-Karnitin (LK) in DNye etkileri araştırıldı. Dokuz ratlıkgruplarda, sağlıklı (SK) ve hastalıklı (HK) kontrol dışındakilere içme suyu ile 2.5 mg/kg gün EN velOO mg/kg/gün LK altı hafta verildi. Çalışma sonunda 24st lik idrar toplandı, kardiak ponksiyonla kan alınarak ratlar sakrifiye edildi. Kanda; eritrosit sayısı (Er), hemoglobin (Hb), hematokrit (Hct), plazmada;glukoz, HbAlC, üre, kreatinin (pKr), ürik asit (ÛA), PO4, Ca2*, Na\K+, albumin (Alb) ve Er içi antioksidan enzim olarak süperoksit dismutase (SOD) ve lipid peroksidasyon ürünü (ROS) malonyldialdehid (MDA) ölçüldü. İdrarla atılan glukoz (UG), albumin (UAE) ve N-acetyl-B-D glukozaminidase (NAG) incelendi. Böbrek dokusu ışık mikroskobunda ve immunohistokimyasal Tip IV kollajen birikimi incelendi. STZileglukoz, HbAlc, UG, UAE, NAG(P<000), üre, pKr (P<.005) artışı ile DN oluştu. HK grubuna göre, EN grubunda UG (P<.000), NAG, idrar volümü ve PO4 düşük (P<.05), Albyüksek (P<.05) veLKgrubunda Er, Hb yüksek, UG, NAG ve idrar volümü düşük (P<.05) bulundu. SK'egöre MDA değeri HK grubunda anlamsız arttı, EN ve LK grubunda değişmedi. DN bulguları olan glomerüler ve tubuler bazal membranda kalınlaşma ve tip IVkollajen birikimiHK'da belirgin, EN'de orta, LKgrubunda hafif derecede bulundu. Bulgularımız, ACEi'ne benzer şekilde LK'in DN gelişimini azaltıcı etki yaptığını düşündürmektedir.
FULL TEXT (PDF): 
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  • 4
223-229
  • Turkish

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