Buradasınız

Anasayfa » Content

Inducible clindamycin resistance among Staphylococcus aureus isolates

Journal Name:

  • Indian Journal of Basic & Applied Medical Research

Publication Year:

  • 2013

Key Words:

Author Name
Abstract (2. Language): 
Introduction: Clindamycin is considered an useful alternate drug in penicillin-allergic patients in the treatment of skin & soft tissue infections caused by Staphylococcus aureus. Staphylococcus spp. can be resistant to erythromycin through either erm or msr A genes. Strains with erm-mediated erythromycin resistance may possess inducible clindamycin resistance but may appear susceptible to clindamycin by disc diffusion test. The objective of the present study was to know the prevalence of erythromycin-induced clindamycin resistance among clinical isolates of S. aureus. Methods: A total of 250 S. aureus isolates from various clinical samples submitted in the Dept. of Microbiology at our tertiary care hospital were studied. Methicillin resistant S. aureus strains were identified by Cefoxitin disc diffusion method. Inducible clindamycin resistance was detected by erythromycin and clindamycin disc approximation test (D-zone test) as per CLSI guidelines. Result: Among the 250 S. aureus isolates, 107 strains (42.8%) were detected as MRSA of which 26(24.3%) strains showed inducible clindamycin resistance (D-test positive). Only 7(5.0%) isolates of MSSA were D-test positive. 156(62.4%) isolates of S. aureus were sensitive to both erythromycin & clindamycin. Conclusion: High prevalence of strains with inducible clindamycin resistance particularly among MRSA indicates that inducible clindamycin resistance testing (D-test) should be included as a part of routine antibiotic susceptibility. These isolates may be missed in routine antibiotic testing by disk diffusion method.
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon 961-967.pdf.pdf
  • 8
961-967
  • English

REFERENCES

References: 

1. Jevons MP. “Celbenin”-resistant staphylococci. Br Med J 1961;1:124-26.
2. Gadepalli R, Dhawan B, Mohanty S, Kapil A, Das BK, Chaudhry R. Inducible clindamycin
resistance in clinical isolates of Staphylococcus aureus. Indian J Med Res 2006;123:571-73.
3. Hwan Sub Lim, Hyukmin Lee, Kyoung Ho Roh and Jong Hwa Yum et al. Prevalence of
Inducible Clindamycin resistance in Staphylococcal Isolates at a Korean Tertiary Care Hospital.
Yonsei Med Journal 2006; 47(4): 480-484.
4. Leclercq R. & Courvalin, P. Intrinsic and unusual resistance to macrolide, lincosamide and
streptogramin antibiotics in bacteria. Antimicrobial Agents and Chemotherapy 1991;35:1273–6.
Indian Journal of Basic & Applied Medical Research; September 2013: Issue-8, Vol.-2, P. 961-967
966
www.ijbamr.com
5. Steward CD, Raney PM, Morrell AK, Williams PP, McDougal LK, Jevitt L, et al. Testing for
induction of clindamycin resistance in erythromycin resistant isolates of Staphylococcus aureus. J
Clin Microbiol 2005;43:1716-21.
6. Upadhya A, Biradar S. The prevalence of inducible clindamycin resistance in Staphylococcus
aureus in a tertiary care hospital in north-east Karnataka, India. Health Sciences: An International
Journal. 2011;1(3):21–24.
7. Performance Standards for Antimicrobial Susceptibility Testing; Twentieth informational
supplement. CLSI document. M100-S20. Pennsylvania: Clinical and Laboratory Standards
Institute; 2011.
8. Mohanasoundaram KM. The prevalence of inducible clindamycin resistance among gram positive
cocci from various clinical specimens. Journal of Clinical and Diagnostic Research 2011;5:38-40.
9. Kasten MJ. Clindamycin, metronidazole, and chloramphenicol. Mayo Clin Proc. 1999;74:825–33
10. Yilmaz G, Aydin K, Iskender S, Caylan R, Koksal I. Detection and prevalence of inducible
clindamycin resistance in staphylococci. J Med Microbiol. 2007;56:342–5.
11. Deotale V, Mendiratta DK, Raut U, Narang P. Inducible clindamycin resistance
in Staphylococcus aureus isolated from clinical samples. Indian J Med Microbiol. 2010; 28:124–
6.
12. Shantala GB, Shetty AS, Rao RK, Vasudeva, Nagarathnamma T. Detection of inducible
clindamycin resistance in clinical isolates of Staphylococcus aureus by the disc diffusion
induction test. Journal of Clinical and Diagnostic Research 2011; 5:35-7.
13. Schreckenberger PC, Ilendo E, Ristow KL. Incidence of constitutive and inducible clindamycin
resistance in Staphylococcus aureus and coagulase-negative staphylococci in a community and a
tertiary care hospital. J Clin Microbiol 2004;42:2777-9.
14. Levin TP, Suh B, Axelrod P, Truant AL, Fekete T. Potential clindamycin Resistance in
clindamycin-susceptible, erythromycin-resistant Staphylococcus aureus: Report of a clinical
failure. Antimicrob Agents Chemother 2005;49:1222-4.
15. Angel MR, Balaji V, Prakash J, Brahmadathan KN, Mathews MS. Prevalence of inducible
clindamycin resistance in Gram positive organisms in a tertiary care centre. Indian J Med
Microbiol 2008;26:262-4.
16. Anbumani N, Kalyani J, Mallika M. Prevalence of methicillin-resistant Staphylococcus aureus in
a Tertiary Referral Hospital in Chennai, South India. Indian Journal for the Practising Doctor
2006-08 - 2006-09;3(4).
17. Tyagi A, Kapil A, Singh P. Incidence of methicillin resistant Staphylococcus aureus (MRSA) in
pus samples at a tertiary care hospital, AIIMS, New Delhi. Journal Indian Academy of Clinical
Medicine 2008;9(1): 33-5.
18. Fridkin SK. Vancomycin-intermediate and resistant Staphylococcus aureus: what the infectious
disease specialist needs to know. Clin Infect Dis 2001; 32:108-15.
Indian Journal of Basic & Applied Medical Research; September 2013: Issue-8, Vol.-2, P. 961-967
967
www.ijbamr.com
19. Bal M, Saha B, Singh AK, Ghosh A. Identification and characterization of a vancomycinresistant
Staphylococcus aureus isolated from Kolkata (South Asia). J Med Microbiol
2008;57:172-79.

Thank you for copying data from http://www.arastirmax.com