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SİYAH ALACA SIĞIRLARINDA İNSÜLİN BENZERİ BÜYÜME FAKTÖRÜ-I (IGF-I) GENİNİN 5’- SINIRDAŞ BÖLGESİNDEKİ TEK NÜKLEOTİD POLİMORFİZMİNİN FERTİLİTE İÇİN POTANSİYEL GENETİK MARKIR OLARAK DEĞERLENDİRİLMESİ

Assessment of Single Nucleotide Polymorphism in the 5'-Flanking Region of Insulin-Like Growth Factor-I (IGF-I) Gene as a Potential Genetic Marker for Fertility in Holstein Dairy Cows

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2013

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Insulin-like growth factor-I (IGF-I) is one of the most important metabolic factors that plays a critical role in cow fertility. Recently, point mutation of IGF-I gene at nucleotide position 512 (C/T transition) within the 5′-untranslated region (5'-UTR) has attracted the most attention. It has previously been demonstrated that there was an important relationship between this type of mutation and IGF-I concentration in dairy cows serum. Hence, we investigate the relationship between polymorphism within 512 of the 5'-UTR of IGF-I gene and some reproductive parameters in Iranian Holstein dairy cows. Blood samples were collected from 60 multiparous cows with a history of desirable fertility (SPC<2; n=25) and undesirable fertility (SPC2; n=35) based on the previous lactation numbers. The results showed that higher percentage (65%) of cows with CC genotype had desirable fertility (SPC<2); while a lower percentage of cows with TC (33.3%) and TT genotypes (14.3%) had good fertility (P<0.05). Therefore, the CC genotype was associated (P<0.05) with desirable fertility. In addition, the IGF-I mutation had a significant effect on service per conception and days open in which cows with TT genotype and TC genotype had greater average of services per conception and days open compared to cows with the CC genotypes (P<0.05). It can be concluded that the C/T mutation within this region of IGF-I gene may influence the reproductive parameters in Holstein dairy cows. These data support the hypothesis that the mutation in this site might be considered as a genetic marker for reproductive performance in Holstein dairy cows.
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Abstract (Original Language): 
İnsulin benzeri büyüme faktörü-I (IGF-I) inek fertilitesinde kritik bir rol oynayan en önemli metabolik faktörlerden birisidir. Son yıllarda IGF-I geninin 5’- kodlanmayan bölgesinde (5’-UTR) 512. nükleotid pozisyonundaki (C/T dönüşümü) nokta mutasyonu oldukça ilgi çekmiştir. Bu tip mutasyon ile süt ineği serumundaki IGF-I konsantrasyonu arasında önemli bir ilişki bulunduğu bildirilmiştir. Bu nedenle İran Siyah Alaca sütçü ineğine ait bazı reprodüktif parametreler ile IGF-I geninin 5'-UTR bölgesinde 512. nükleotidindeki polimorfizm arasındaki ilişkiyi inceledik. Kan örnekleri, daha önceki laktasyonlarında istenilen düzeyde fertilite gösteren (SPC<2; n = 25) ve göstermeyen (SPC2; n=35), daha önce buzağılamış toplam 60 adet inekten toplanmıştır. İneklerin yüksek bir yüzdesi (%65) CC genotipine ve istenilen fertilite düzeyine sahip oldukları (SPC<2); ineklerin daha düşük bir yüzdesinin de (%33) TC ve TT (%14,3) genotiplerine ve iyi fertiliteye sahip oldukları (P<0,05) tespit edilmiştir. Bu nedenle CC genotipi istenilen fertilite düzeyi ile bağlantılıdır (P<0,05). Ayrıca, IGF-I mutasyonunun gebelik başına tohumlama sayısı ve servis periyodu süresi üzerine önemli derecede etkili olduğu bulunmuştur. TT ve TC genotipine sahip ineklerin CC genotipine sahip olanlara göre daha yüksek gebelik başına tohumlama sayısı ve servis periyodu süresi ortalamasına sahip oldukları tespit edilmiştir (P < 0,05). Bu çalışmada sonuç olarak IGF-I geninin bu bölgesinde meydana gelen C/T mutasyonu Siyah Alaca ineklerde reprodüktif parametreleri etkileyebileceği sonucuna ulaşılmıştır. Elde edilen sonuçlar, bu bölgedeki mutasyonun Siyah Alaca ineklerde genetik işaretleyici olarak kullanılabileceği hipotezini desteklemektedir.
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