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EVALUATION OF ANTIOXIDANT ACTIVITY, PHENOL AND FLAVONOID CONTENTS OF MOMORDICA CHARANTIA LINN. FRUIT

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Abstract (2. Language): 
In present study, we carried out antioxidant activity, phenol and flavonoid contents of momordica charantia Fruit extracts. Total phenolic content in the alcoholic extract of M. charantia extract was found to be 5.61 ± 0.54 % w/w calculated in terms of Gallic acid. The total flavonoids content of aqueous extract of M. charantia Linn was found to be 1.77 ± 0.72 % w/w was expressed as equivalent to Rutin. The Momordica Charantia fruit were successively extracted using soxhlet apparatus with solvent petroleum ether (60-80 0C), chloroform, alcohol & water. These extracts were subjected to the DPPH radical scavenging & hydrogen peroxide radical scavenging activity was determined spectrophotometrically. The IC50 values of alcoholic extract in DPPH & Hydrogen Peroxide radical scavenging activity was found to be 120.07 ± 0.77μg/ml & 175.78 ± 0.63 μg/ml respectively. The highest radical scavenging effect was observed in alcoholic extract of M. charantia Linn. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by M. charantia Linn. extract.
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