Buradasınız

Anasayfa » Content

HPTLC METHOD DEVELOPMENT AND ESTIMATION OF QUERCETIN IN THE ALCOHOLIC EXTRACT OF AERVA JAVANICA ROOT

Journal Name:

  • Advance Research in Pharmaceuticals and Biologicals

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
Aerva javanica belonging to family Amaranthaceae was selected for the present study. High performance thin layer liquid chromatography (HPTLC) was more popular in quality control and standardization of traditional herbs. The present study is to develop chromatography fingerprinting of Aerva javanica. A new, economic and rapid high performance thin-layer chromatographic method was developed for alcoholoic extract of root of Aerva javanica. Chromatographic separation was achieved on a pre-coated with silica gel 60 F254 plate using a mixture of toluene: acetone: methanol: formic acid (7: 2: 0.8: 0.2 v/v) as a mobile phase at a wavelength of 254 nm and 366 nm. Derivatization was done with anisaldehyde sulphuric acid as a detecting reagent. Developed HPTLC method can be used for determination of quercetin. The minimum detectable amount of quercetin was found to be 0.02196% w/w. Hence, the present method was successfully employed for the estimation of quercetin in the alcoholoic extract of root of Aerva javanica.
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon arastrmx_160852_2_pp_222-228.pdf
  • 2
222-228
  • English

REFERENCES

References: 

1. B. Vaidya. Some Controversial Drugs
in Indian Medicine. First ed.
Chaukhambha Orientalia, Varanasi,
1982, pp. 2-5.
2. R. K. Gupta, Y. D. Gaur, S. P.
Malhotra and B. K. Dutta. Medicinal
plants of the indian arid zone. Journ.
d'Agric. trop. et de Bot. Appl. 13(6-7):
247-288 (1966).
3. W. Dymock, C. J. H. Warden and D.
Hooper. Pharmacographia indica,
Kegan Paul, Trench, Trubner and Co.
Ltd., London, Vol. III, 1890, pp. 135-
158.
4. R. N. Chopra, I. C. Nayar and I. C.
Chopra. Glossary of Indian Medicinal
Plants,Council of Scientific and
Industrial Research, New Delhi, 1956,
pp. 4-8.
5. K. R. Kirtikar and B. D. Basu. Indian
Medicinal Plants, Indian Press,
Allahabad, Vol. III, 1918, pp. 2064-
2068.
6. S. R. Baquar. Medicinal and Poisonous
Plants of Pakistan. Printas Karanchi,
1989, pp.7-8, 15-16.
7. A. T. Gaze. The vegetation of the
district of Minbu in Upper Burma,
Rec. Bot. Surv. India, 3, 1940, pp. 139.
8. S. P. Garg, R. Bhushan and R. C.
Kapoor. Chrysin-7-O-galactoside: a
new flavanoids from Aerva persica
burm.f. Indian J. Chem. Section-B, 17
B (4): 416-417 (1979).
9. N. A. Saleh, R. M. Mansour and K. R.
Markham. An Acylated isorhamnetin
glycoside from Aerva javanica.
Vinit et al., ARPB, 2012; Vol 2(II) ISSN 2250 - 0744
(Research Article)
www.arpb.info Page 228
Phytochemistry 29(4): 1344-1345
(1990).
10. V. L. Singleton and J. A. Rossi.
Colorimetry of total phenolics with
phosphomolybdic acidphosphotungstic
acid reagents. Am. J.
Enol. Viticult. 16: 144-158 (1965).
11. A. Jain, S. Lodhi and A. K. Singhai.
Simultaneous estimation of quercetin
and rutin in Tephrosia purpurea Pers
by high performance thin-layer
chromatography. Asian Journal of
Traditional Medicines, 4(3): 104-109
(2009).
12. A. Kumar, K. Lakshman, K. N.
Jayaveera, S. N. M. Tripathi and K. V.
Satish. Estimation of Gallic acid in
Terminalia chebula by hptlc. Jordan
Journal of Pharmaceutical Sciences,
3(1): 63-67 (2010).

Thank you for copying data from http://www.arastirmax.com