Journal Name:
- Advance Research in Pharmaceuticals and Biologicals
Key Words:
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Abstract (2. Language):
Aerva javanica belonging to family Amaranthaceae was selected for
the present study. High performance thin layer liquid
chromatography (HPTLC) was more popular in quality control and
standardization of traditional herbs. The present study is to develop
chromatography fingerprinting of Aerva javanica. A new, economic
and rapid high performance thin-layer chromatographic method was
developed for alcoholoic extract of root of Aerva javanica.
Chromatographic separation was achieved on a pre-coated with
silica gel 60 F254 plate using a mixture of toluene: acetone:
methanol: formic acid (7: 2: 0.8: 0.2 v/v) as a mobile phase at a
wavelength of 254 nm and 366 nm. Derivatization was done with
anisaldehyde sulphuric acid as a detecting reagent. Developed
HPTLC method can be used for determination of quercetin. The
minimum detectable amount of quercetin was found to be 0.02196%
w/w. Hence, the present method was successfully employed for the
estimation of quercetin in the alcoholoic extract of root of Aerva
javanica.
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