Journal Name:
- Advance Research in Pharmaceuticals and Biologicals
Key Words:
Abstract (2. Language):
The present work deals with a simple and accurate liquid chromatography mass spectrometry method for the
simultaneous estimation of Nevirapine and Lamivudine from their combination drug product with carbamazepine as
internal standard. The proposed LC-MS/MS method utilizes HyPURITY ADVANCE 4.6x50 mm, 5 microm (make thermo)
column, at ambient temperature, optimum mobile phase consists of methanol: 5M ammonium acetate buffer (85;
15v/v) and flow rate monitored at 0.800 ml/min. the linearity of the proposed method was investigated in the range of
25.45ng/ml to 4990.23 mg/ml concentrations for Nevirapine and 15.30 mg/ml to 2999.63 mg/ml concentrations for
Lamivudine1. The mean recoveries were 93.58% and 91.73% for Nevirapine and Lamivudine, respectively. The
proposed method was validated for precision, accuracy, linearity, range, robustness and ruggedness. All validation
parameters were within the acceptable range. The developed method was successfully applied to estimate the amount
of Nevirapine and Lamivudine in combined dosage forms.
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FULL TEXT (PDF):
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283-289