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PRODUCTION OF AMYLASE FROM EICHHORNIA CRASSIPES AND PISTIA STRATIOTES AS SUBSTRATES USING SOLID STATE FERMENTATION

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Abstract (2. Language): 
B. Subtilis (NCIM 2063) and A. Niger (NCIM 620) were used for amylase production from E. Crassipes and P. Stratiotes. The fermentation batches were set up in minimal basal media. Maximum amylase activity was seen by Aspergillus niger on Pistia stratiotes (Day 2) and Eicchornia crassipes (Day 4) at 32°C. A. niger with a spore count of 6.4x109 spores/ml after partial purification produced 6.8 units/ml enzyme in P. stratiotes and 7.3units/ml enzyme in E. crassipes with a spore count of 3.2x109 spores/ml. B. subtilis with CFU of 4.1x109 after partial purification produced 6.239 units/ml enzyme in P. stratiotes and 6.08 units/ml enzyme in E. crassipes with CFU of 4.6x109. The results obtained are significant as there have been no reports of production of amylase from these two plants using solid state fermentation (SSF).
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