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FUSARİUM OXYSPORUM F.SP. CİCERİS' İN FUSARİK ASİT ÜRETİMİNİN İNCE TABAKA KROMATOGRAFİSİ VE SPEKTROFOTOMETRİK METODLAR İLE BELİRLENMESİ

DETERMINATION OF FUSARIC ACID PRODUCTION BY FUSARIUM OXYSPORUM F.SP. CICERIS WITH THIN LAYER CHROMATOGRAPHY AND SPECTROPHOTOMETRIC METHODS

Journal Name:

  • Anadolu Tarım Bilimleri Dergisi

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
This study is performed to determine whether a relation exists between fusaric acid production and virulence of Fusarium oxysporum f.sp. ciceris isolates. Five isolates (KC 7, A4, KA6, KC9 ve KC6) of F. oxysporum f.sp. ciceris were grown in Czapek Dox liquid medium for 5, 10, 15 and 20 days and toxins were extracted by solid phase extraction using C18 catridge. Then, toxins were eluted with acetonitrile. The presence of fusaric acid in acetonitrile was confirmed by both thin-layer chromatography and spectrophotometer. Thin-layer chromatography of fusaric acid gave a fluoresent brick colored spot at Rf 0.70. In addition to that, an unknown metabolite was found in thin-layer chromatography analysis (Rf 0.87). Quantitave analysis of fusaric acid was performed by Agilent Specord 200 spectrophotometer. None of the isolates produced fusaric acid in the 5th day. Also, differences in fusaric acid production between low and high virulent isolates were found as statistically significant in the 20th day of incubation.
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Abstract (Original Language): 
Bu çalışma Fusarium oxysporum f.sp. ciceris izolatlarının fusarik asit üretimi ve virülenslikleri arasında bir ilişki olup olmadığını belirlemek için yürütülmüştür. F. oxysporum f.sp. ciceris' in beş izolatı (KC 7, A4, KA6, KC9 ve KC6) Czapek Dox sıvı besin ortamında 5, 10, 15 ve 20 gün süreyle geliştirilmiş ve toxin C18 katı faz ekstraksiyon kartijleriyle ekstrakte edilerek asetonitril ile elue edilmiştir. Asetonitrildeki fusarik asitin varlığı hem ince tabaka kromatografi hem de spektrofotometrik yöntemle belirlenmiştir. Fusarik asitin ince tabaka kromatorafisinde Rf değeri 0.70 olarak bulunmuş ve fluoresan kremit rengi parlaklıkta bir leke vermiştir. Buna ilave olarak ince tabaka kromatografi analizinde bir izolatın tanımlanamayan bir metabolit ürettiği belirlenmiştir (Rf 0.87). Fusarik asitin kantitatif miktar analizi Agilent Specord 200 spectrofotometre ile gerçekleştirilmiştir. İzolatların hiçbirisi inkübasyonun 5. gününde fusarik asit üretmemiştir. Ayrıca düşük ve yüksek virülens izolatların fusarik asit üretimindeki farklılıklar inkübasyonun 20. gününde istatiksel olarak önemli bulunmuştur.
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  • 3
146-150
  • Turkish

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