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POLİMERAZ ZİNCİR REAKSİYONU (PCR) VE ENDODONTİK MİKROBİYOLOJİ

POLYMERASE CHAİN REACTION (PCR) AND ENDODONTIC MICROBIOLOGY

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2006

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
This article reviews the use of polymerase chain reaction (PCR) in endodontics and the advantages nn detecting the presence and prevalence of endodontic microorganisms. Amplification of 16S rRNA gene regions of the uncultivable and fastidious microorganisms by PCR has baazed a trail nn molecular microbiology. PCR is more specific, accurate, rapid and sensitive than culture methods. The well-directed use of these methods will provide additional valuable information regarding the identification and understanding of the causative factors associated with endodontic diseases, and may contribute to the development of improved treatment strategies.
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Abstract (Original Language): 
Bu makale polimeraz znncrr reaksiyonunun (Polymerase Chain Reaction-PCR) endodontide kullanımını, endodontik mikroorganizmaların varlığının ve prevalanslarının belirlenmesinde sağladığı avantajları gözden geçirmektedir. Küttür yöntemiyle üretilemeyen mikroorganizma/arın 16S rRNA bölgelerinin PCR kul/anılarak çoğaltılması mo/ekü/er mikrobiyolojide çığır açmıştır. PCR kültür yöntem/erine göre daha hassas, özgül, hızlıdır ve daha kesin sonuç/ar sağlar. Bu metodların yerinde kullanımı, endodontik hastaııkların nedenlerinin belirlenmesine, anlaşılmasına ve tedavi stratejilerinin geliştirilmesine önemli katkılar sağlayacaktır.
FULL TEXT (PDF): 
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  • 2
61-65
  • Turkish

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