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FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ

IN VITRO EVALUATION OF THE CYTOTOXIC EFFECTS OF ROOT CANAL SEALE RS WITH DIFFERENT CHEMICAL COMPOSITIONS

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The present study aimed to compare different canal sealers in different setting times in terms of the cytotoxicity they induce on 3T3 mouse fibroblasts. AH Plus, AH 26, Endomethasone N, RSA and Sealapex were put into glass moulds in 2 mm diameter and 10 mm length prepared previously after mixing under sterilized condition according to manufacturer's instructions. Test samples were divided into three groups; in the first group canal sealers were freshly prepared and in the others, canal sealers were set for 3-days and 7-days. Samples with different setting times were diluted in 10 ml culture medium for extraction periods of 1, 4, and 7 days. Extracts were taken after each dilution period, sterilized by 0.22 um filter, diluted 1:1, 1:2, 1:4, 1:8, and applied to 3T3 mouse fibroblasts during one-day incubation. MTS (tetrazolium salt) analysis was performed to evaluate viable cell rates. Different variables included in our study were statistically evaluated both in relation to each other and independently in terms of the cytotoxicity they induced. The results confirmed that all the sealer samples and each setting and extraction times, and each dilution showed statistically significant differences in relation to each other (p<0,05). Canal sealers were listed as Sealapex > AH 26 > Endomethasone N > AH Plus > RSA according to their cytotoxicity levels. Furthermore, it was observed that as setting time and dilution rate increase, cytotoxicity decreases, while the longer the extraction time, the greater the cytotoxicity.
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Abstract (Original Language): 
Bu çalışmamızın amacı, farklı sertleşme sürelerine sahip değişik içerikli kanal patlarının, 3T3 fare fibroblastları üzerinde oluşturdukları sitotoksisitenin in vitro olarak incelenmesi ve karşılaştırılmasıdır. AH Plus, AH 26, Endomethasone N, RSA ve Sealapex üreticilerinin talimatları doğrultusunda steril şartlarda karıştırılarak önceden hazırlanan 2 mm çapında ve 10 mm uzunluğundaki standart steril cam kalıplara yerleştirildi. Test örnekleri, taze, 3. gün ve 7. günde değerlendirilmek üzere 3 gruba ayrıldı. Farklı sertleşme zamanına sahip örnekler, 1 günlük, 4 günlük ve 7 günlük ekstraksiyon periyotları için 10 ml'lik kültür ortamda dilüe edildiler. Her dilüsyon periyodundan sonra ekstre alındı, 0.22 um lik filtrede steril edildi ve 1:1, 1:2, 1:4, 1:8 oranlarında seyreltilerek, 1 günlük inkübasyon süresince 3T3 fare fibroblastlarına uygulandı. Yaşayabilen hücre oranları MTS (tetrazolium salt) analizi yapılarak değerlendirildi. Araştırmamızı oluşturan çeşitli değişkenler, oluşturdukları sitotok-sisiteye göre, hem kendi aralarında hem de kendi içlerinde istatistiksel olarak değerlendirildi. Araştırmamızda, her kanal patının, her sertleşme ve ekstraksiyon süresinin ve her seyreltme oranının kendi aralarında istatistiksel olarak önemli farklılıklar gösterdiği bulundu (p<0,05). Kanal patları, sitotoksisite derecelerine göre, Sealapex > AH 26 > Endomethasone N > AH Plus > RSA olarak sıralandı. Ayrıca çalışmada sertleşme zamanı ve seyreltilme oranı artıkça, sitotoksisite azalırken, ekstraksiyon süresi uzadıkça sitotoksisitenin arttığı görüldü.
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  • 2
90-97
  • Turkish

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