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ANADOLU'NUN ÜÇ FARKLI BÖLGESİNDEN ALINAN PROPOLİS ÖRNEKLERİNİN ANTİMİKROBİYAL AKTİVİTELERİNİN İN VİTRO OLARAK İNCELENMESİ

IN VITRO ANTIMICROBIAL ACTIVITY OF PROPOLIS SAMPLES FROM THREE DIFF ERENT REGIONS OF ANATOLIA

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2011

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The aim of this study was to evaluate the antimicrobial activity of propolis samples from Zara/Sivas, Trabzon and Aydın regions in Turkey by the agar diffusion method. Antimicrobial activities of different propolis extracts (10 and 20%) were tested against E. faecalis, S. aureus, E. coli and C. albicans, in the present study. Microorganisms were inoculated into plates containing Mueller Hinton (MH) agar. Then round wells were punched in the cultivated agar plates and filled with test solutions. The MH agar plates were incubated for 24 h at 37°C and diameters of inhibition zones around each well were then measured and recorded. The test was repeated 7 times for each solution. The results of the present study showed that 2.5% sodium hypochlorite (NaOCl) and all propolis extracts showed antimicrobial activity against all microorganisms tested. However, 2.5% NaOCl was shown to be the most effective irrigant and statistically significant differences were found between 2.5% NaOCl and other experimental solutions against all microorganisms (p<0.05). When propolis extracts were evaluated, Zara propolis extracts showed better antimicrobial activity than other propolis extracts against all microorganisms tested. Also, there were statistically significant differences between 10 and 20% concentrations of propolis extracts from Zara and other propolis extracts against E. faecalis and C. albicans (p<0.05). Propolis extracts from Aydın showed better antimicrobial activity than Trabzon propolis extracts against C. albicans and E. faecalis Also, significant differences were found between 10% concentration of propolis extract from Trabzon and 20% concentration of propolis extract from Aydın against S. aureus (p<0.05).
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Abstract (Original Language): 
Bu çalışmanın amacı, Türkiye'nin Zara/Sivas, Trabzon ve Aydın bölgelerinden alınan propolis örneklerinin, agar difüzyon yöntemi ile antimikrobiyal aktivitelerinin incelenmesidir. Çalışmada, farklı propolis ekstratlarının (%10 ve %20) E. faecalis, S. aureus, E. coli ve C. albicans üzerine olan antimikrobiyal aktiviteleri test edildi. Mikroorganizmalar Mueller Hinton (MH) agar içeren plaklara ekildi. Daha sonra ekim yapılan agar plaklarına kuyucuklar açıldı ve test solüsyonları ile dolduruldu. MH agar plakları 37°C'de 24 saat inkübe edildi ve inhibisyon alanlarının genişlikleri ölçülerek kaydedildi. Her solüsyon için bu test 7 kere tekrarlandı. Çalışmanın sonucunda, %2,5'lik sodyum hipoklorit (NaOCl) ve tüm propolis ekstratlarının test edilen mikroorganizmalara karşı antimikrobiyal aktivitesinin olduğu görüldü. Bununla birlikte, %2,5'lik NaOCl'nin en etkili irrigan olduğu ve tüm mikroorganizmalara karşı diğer solüsyonlarla arasında istatistiksel olarak fark olduğu görüldü (p<0,05). Propolis ekstratları değerlendirildiğinde, Zara propolis ekstratlarının diğer propolis ekstratlarına göre test edilen bakterilere daha iyi antimikrobiyal etki gösterdiği bulundu. E. faecalis ve C. albicansa karşı %10 ve %20 konsantrasyonlardaki Zara propolis ekstratları ile diğer propolis ekstratları arasında istatistiksel olarak fark vardı (p<0,05). Aydın propolis ekstratları, E. faecalis ve C.albicans üzerine Trabzon propolis ekstratlarına göre daha iyi antimikrobiyal etki gösterdi. Ayrıca, %10'luk Trabzon ve %20'lik Aydın propolis ekstratları arasında S.aureusa karşı önemli fark bulundu (p<0,05).
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  • 2
82-87
  • Turkish

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