Buradasınız

Anasayfa » Content

ATATÜRK ÜNİVERSİTESİ DİŞ HEKİMLİĞİ FAKÜLTESİNE BAŞVURAN BİREYLERİN AEROBİK BAKTERİYEL FLORASININ MİKROBİYAL TEŞHİS SİSTEMİ KULLANILARAK SAPTANMASI

IDENTIFICATION OF AEROBIC BACTERIAL FLORA IN SALIVA OF SUBJECTS WHO APPLY TO THE FACULTY OF DENTISTRY IN ATATÜRK UNIVERSITY BY USING MICROBIAL IDENTIFICATION SYSTEM

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Introduction: The aim of the present study was to identify the aerobic bacterial flora in the saliva of patient who apply to The Faculty of Dentistry in Atatürk University. Methods: Unstimulated saliva samples were collected from 20 healthy subjects with Decayed Missed Filled Teeth (DMFT) index values of between 9-15. Total bacterial flora was recovered from saliva samples on two non-selective media, Nutrient Agar and Sensitive Agar. Isolated bacterial organisms were identified based on fatty acid methyl ester (FAME) analysis using the Microbial Identification System (MIS; MIDI Inc., Newark, Del.). Results: A total of 75 bacterial strains were isolated. Of these, 67 were identified as belonging to 34 species of 16 genera, while the remaining strains were unidentified. The most abundant bacterial genus was Streptococcus, followed by Bacillus, Neisseria, Psychrobacter, Enterecoccus, Haemophilus, Staphylococcus, Paenibacillus, Camphylobacter, Micrococcus, Xanthobacter, Helicobacter, Actinomadura, Kocuria, Pseudomonas, and Cellulomonas. To our knowledge, this is the first report in the literature of the identification of the bacterial species Xanthobacter agilis, Actinomadura yumaensis and Psychrobacter-phenylpyruvicus in oral flora. Conclusions: This is the first study to utilize MIS for the identification of aerobic bacteria in saliva. Our results suggest that MIS is an accurate, efficient and relatively rapid method for identifying bacteria in saliva; however, the MIS databases, particularly those of oral bacterial flora, need to be improved.
Bookmark/Search this post with
Abstract (Original Language): 
Bu çalışmanın amacı, Atatürk Üniversitesi Diş Hekimliği Fakültesine başvuran hastaların tükürük aerobik bakteri florasını tanımlamaktır. Yöntem: DMFT indeksi 9-15 arasındaki 20 sağlıklı bireyin sitümüle edilmemiş tükürükleri toplandı.Toplam bakteri florası iki seçici olmayan medya, Nutrient Agar ve Sensitive Agar üzerindeki tükürük örneklerinden toplanmıştır. Izole bakteriyel organizmalar, fatty acid methyl ester (FAME) analizine dayanan Microbial Identification System (MIS; MIDI Inc., Newark, Del.) kullanılarak sınıflandırıldı . Sonuçlar. 75 bakteri suş izole edildi. Bunlardan, 67’sinin , 16 cins ve 34 türe ait olduğu saptanırken Kalan suşlar tanımlanamadı. En çok bulunan bakteri Streptococcus iken takip edenler Bacillus, Neisseria, Psychrobacter, Enterecoccus, Haemophilus, Staphylococcus, Paenibacillus, Camphylobacter, Micrococcus, Xanthobacter, Helikobakter, Actinomadura, Kocuria, Pseudomonas ve Cellulomonas oldu. Bilgilerimize dayanarak bu çalışma, bakteri türlerinden Xanthobacter Agilis, Actinomadura yumaensis ve Psychrobacter-phenylpyruvicus’u ağız florasında tanımlayan ilk literatürdür. Sonuç: Bu Çalışma;tükürük aerobik bakterilerinin tespiti için MIS’den yararlanılan ilk çalışmadır. Bizim sonuçlarımız MIS’in tükürük bakterinin tespiti için verimli ve hızlı ve doğru bir yöntem olduğunu desteklemektedir; ancak MIS veritabanlarının özellikle ağız bakteri florası olanlarının geliştirilmesi gerekmektedir.
FULL TEXT (PDF): 
PDF icon arastirmax-ataturk-universitesi-dis-hekimligi-fakultesine-basvuran-bireylerin-aerobik-bakteriyel-florasinin-mikrobiyal-teshis-sistemi-kullanilarak-saptanmasi.pdf
  • 1
26-30
  • Turkish

REFERENCES

References: 

1. Marcotte H, Lavoie MC. Oral microbial ecology and
the role of salivary immunoglobulin A. Microbiol
Mol Biol Rev 1998; 62: 71-109.
2. Thylstrup A, Fejerkov O. Textbook of Clinical
Cariology. 2 ed.Copenhagen; Munsgaard:1994.p.
5-50.
3. Gabris K, Nagy G, Madlena M, Denes Z, Marton S,
Keszthelyi G, Banoczy J. Associations between
microbiological and salivary caries activity tests
and caries experience in Hungarian adolescents.
Caries Res 1999; 33:191-195.
4. Tukia-Kulmana H, Tenovuo J: Intra- and interindividual
variation in salivary flow rate, buffer
effect, lactobacilli, and mutans streptococci among
Atatürk Üniv. Diş Hek. Fak. Derg. ÖZDABAK, KARAOĞLANOĞLU,
J Dent Fac Atatürk Uni AKGÜL, SEVEN
Cilt:22, Sayı: 1, Yıl: 2012, Sayfa: 26-30
30
11-to 12-year-old school children. Acta Odontol
Scand 1993; 51: 31-37.
5. Beighton D, Manji F, Baelum V, Fejerskov O,
Johndon Nw, Wilton JMA. Associations between
salivary levels of streptococcus mutans,
streptococcus sobrinus, lactobacilli, and caries
experience in Kenyan adolescents. J Dent Res
1989; 68:1242-1246.
6. Angulo M, Gabanas B, Camporeale N, Emilson CG.
Dental caries and caries-associated
microorganisms in Uruguayan preschool children.
Acta Odontol Scand 1999; 57: 301-305.
7. Liena-Puy MC, Montanana-Llorens C, Forner-
Navarro L. Cariogenic oral flora and its relation to
dental caries. J Dent Child 2000; Jan-feb: 42-46.
8. Forbes BA, Sahm DF, Weissfeld AS. Bailey and
Scott’s Diagnostic Microbiology.11 ed. St. Louis,
Missouri, USA ; Mosby Inc :1998.p. 1068 .
9. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams
ST. Bergey’s Manual of Determinative Bacteriology
.9 ed. Baltimore, Maryland, USA; Lippincott
Williams & Wilkins: 1994.p 787.
10. Odumeru JA, Steele M, Fruhner L, Larkin C, Jiang
JD, Mann E, McNab WB. Evaluation of accuracy
and repeatability of identification of food-borne
pathogens by automated bacterial identification
systems. J Clin Microbiol 1999; 37: 944-949.
11. Miller I, Berger T. Bacteria identification by gas
chromatography of whole cell fatty acids.
Hewlett-Packard Gas Chromatography Application
Note 228-238. Hewlett-Packard Co. Alto,
CA:1985.p 8.
12. Sahin F. Detection, identification and
characterization of strains of Xanthomonas
campestris pv. vesicatoria by traditional and
molecular methods, and resistance in Capsicum
species to Xanthomonas campestris pv. vesicatoria
pepper race 6. The Ohio State University, 182 p.
(Ph.D. Thesis).
13. Sasser M. Identification of bacteria through fatty
acid analysis. in Methods in Phytobacteriology. Z.
Klement, K. Rudolph, and D. Sands, eds. Budapest
;Akademiai Kiado: 1990.p.199-204.
14. Sherlock Microbial Identification System (version
4.0), MIS Operating manual, MIDI, inc, Newyork,
DE, USA,p 145 .
15. Yang P, Vauterin L, VanCanneyt M, Swings J,
Kersters K. Application of fatty acid methyl esters
for the taxonomic analysis of the genus
Xanthomonas. Syst Appl Microbiol 1993; 16:
47-71.
16. Stead DE. Identification of bacteria by
computer-assisted fatty acid profiling. Acta Hortic
1988; 225: 39-46.
17. World Health Organizations. Oral Health Surveys-
Basic Methods.4th edition. Geneva: WHO: 1997.
18. Gudkina J, Brinkmane A .Caries experience in
relation to oral hygiene, salivary cariogenic
microflora, buffer capacity and secretion rate in 6-
year olds and 12 year olds in RigaStomatologija,
Baltic Dental and Maxillofacial Journal 2008;10:76-
80.
19. Willcox MDP, Drucker DB, Green RM. In vivo
dental plaque-forming ability and cariogenicity of
the bacterium Streptococcus bovis in gnotobiotic
rats. Arch Oral Biol 1990;35:163-166.
20. Crawford I, Russell C. Streptococci isolated from
the bloodstream and gingival crevice of man. J.
Med. Microbiol. 1986;16, 263-269.
21. De Soet JJ, Nyvad B, Kilian M. Strain-related acid
production by oral srteptococci. Caries Res 2000;
34: 486-490.
22. Brailsford SR, Byrne RW, Adams S, Zoitopoulos L,
Allison C, Beighton D. Investigation of the aciduric
microflora of plaque. Caries Res 1999; 33: 290.
23. Rubinstein I, Pedersen GW. Bacillus species are
present in chewing tobacco sold in the United
States and evoke plasma exudation from the oral
mucosa. C

Thank you for copying data from http://www.arastirmax.com