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Mangan (Mn2+) ile kontamine olmuş domates (Lycopersicum esculentum) bitkisinde reaktif oksijen türlerinin (ROS) detoksifikasyonunda katalazın (CAT) rolü

A role of catalase (CAT) in detoxification of reactive oxygen species (ROS) in tomato (Lycopersicum esculentum) contaminated with manganese (Mn2+)

Journal Name:

  • Biological Diversity and Conservation

Publication Year:

  • 2013

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Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In the current study, tomato (Lycopersicum esculentum L.) plants were exposed to various concentrations of Mn2+ (80, 160, 320, 640 and 1280 μM) cation. In the first part, in order to obtain evidence that plants were in stress, following the Mn2+ treatments, lipid peroxidation were determined. In the second part, to gain an idea about regulation of the CAT gene product, enzyme activities were also recorded in Mn2+ treated tomato plants. The maximum lipid peroxidation level was determined in plants which were exposed to 1280 μM concentration of Mn2+ contamination. Changes in lipid peroxidation and CAT enzyme activities in tomato plants exposed to different concentration of Mn2+ contamination revealed no positive correlation. The current study revealed that, ROS induced lipid peroxidation level has depended on concentration of Mn2+ contamination. Also, antioxidant responses to Mn2+ contamination could be reflected as changes in CAT enzyme activity in tomato plants.
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Abstract (Original Language): 
Bu çalışmada, domates bitkileri (Lycopersicum esculentum L.) çeşitli konsantrasyonlardaki (80, 160, 320, 640 ve 1280 μM) Mn2+ katyonuna maruz bırakılmıştır. Çalışmanın ilk bölümünde Mn2+ stresini takiben bitkilerin stres etkisine gitmiş olduklarını göstermek için lipit peroksidasyonu düzeyleri belirlenmiştir. İkinci bölümde CAT gen ürününün regülasyonu değerlendirmek için Mn2+ stresli domates bitkilerinde enzim aktivitesi ölçülmüştür. En yüksek lipit peroksidasyonu 1280 μM konsantrasyonda Mn2+ stresine maruz kalan bitkilerde gözlenmiştir. Farklı konsantrasyonlardaki Mn2+ stresi altındaki domates bitkisinde lipit peroksidasyonu ve CAT enzim aktivitesi düzeyleri arasında pozitif bir korelasyona rastlanmamıştır. Bu çalışma ile ROS ile tetiklenen lipit peroksidasyonu seviyesinin Mn2+ stresinin konsantrasyonuna bağlı olduğu belirlenmiştir. Aynı zamanda Mn2+ stresine karşı oluşan antioksidan cevapların domates bitkisinde CAT enzim aktivitesinin değişimi olarak yansıyabildiği gözlenmiştir.
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