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Domates Bitkisinde (Lycopersicum esculentum L.) Kurşun Kirliliği ile İndüklenen Genotoksisitenin Moleküler ve Populasyon Markörleri ile Değerlendirilmesi

Assesment of genotoxicity induced by lead pollution in tomato (Lycopersicum esculentum) by molecular and population markers

Journal Name:

  • Biological Diversity and Conservation

Publication Year:

  • 2015

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Heavy metal contamination is an important environmental problem that may lead to alterations in vital growth processes, mineral nutrition, transpiration, photosynthesis, enzyme activity and nucleic acids. The effects of environmental pollutants on plants can be monitored using various techniques at different levels. Random amplified polymorphic DNA (RAPD) is a semi-quantitative technique that has been used for genetic mapping, taxonomy, phylogeny and the detection of various kinds of DNA damage and mutations that result from genotoxic agents such as heavy metal contamination. In this study, tomato (Lycopersicum esculentum L.) seeds that had germinated in various concentrations of Pb(NO3)2 solutions were used for measuring population parameters such as dry weight, total soluble protein content, root length and, ultimately, inhibitory rate (IR) values. The seeds were also used to determine the genotoxic effect of the lead, reflected as the appearance or disappearance of bands in RAPD profiles. Inhibition or activation of root elongation was found to be the first effect of metal toxicity to show up in the plants that were tested. Also, total soluble protein content was significantly affected by increased Pb2+ concentrations. The data obtained from RAPD band profiles and genomic template stability (GTS) revealed results that were consistent with the population parameters.
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Abstract (Original Language): 
Ağır metal kirliliği bitkilerin gelişim süreci, mineral beslenme, terleme, fotosentez, enzim aktivitesi ve nükleik asit sentezi gibi bir çok alanda farklılaşmalara sebep olan çevresel bir problemdir. Çevresel kirleticilerin bitkiler üzerindeki etkileri çeşitli teknikler kullanılarak belirlenebilir. Rastgele çoğaltılmış polimorfik DNA (RAPD) yöntemi genetik haritalama, taksonomi, filogeni ve ağır metal kontaminasyonu gibi genotoksik ajanların neden olduğu çeşitli DNA hasarları veya mutasyonlarının belirlenmesinde kullanılan yarı-nicel bir tekniktir. Bu çalışmada, domates (Lycopersicum esculentum L.) tohumları farklı konsantrasyonlardaki Pb(NO3)2 solüsyonu içerisinde çimlendirilmiş ve bu tohumların kuru ağırlık, toplam çözünebilir protein içeriği, kök uzunluğu ve sonuçta engelleyici oranı (IR) belirlenmiştir. Tohumlar aynı zamanda kurşun solüsyonunun RAPD profilinde yeni bir band oluşumu veya kayboluşu olarak akseden genotoksik etkisini belirlemek amacıyla kullanılmıştır. Kök uzamasının inhibisyonu veya aktivasyonu bitkilerde metal toksisitesinin ilk belirtisi olarak gözlenmiştir. Aynı zamanda toplam çözünebilir protein içeriği artan Pb2+ konsantrasyonundan önemi oranda etkilenmiştir. RAPD band profillerinden ve genomik kalıp stabilitesinden (GTS) elde edilen veriler populasyon parametreleri ile uyumludur.
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