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Yeni keşfedilen Coleus forskohlii tıbbi bitkisinde forskolinin teşhisi için yaprak anatomisi ve tüy morfolojisinin farmakognozik değeri

Pharmacognostic value of leaf anatomy and trichome morphology for identification of forskolin in a novel medicinal plant Coleus forskohlii

Journal Name:

  • Biyolojij Çeşitlilik ve Koruma

Publication Year:

  • 2011

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Keywords (Original Language):

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Abstract (2. Language): 
Forskolin, a labdane diterpene is the active principle of the indigenous medicinal plant Coleus forskohlii Briq. It is the only known natural source of the diterpenoid forskolin. Forskolin has a unique property of activating almost all hormone sensitive adenylate cyclase enzymes in a biological system. The pharmacological and biochemical investigations established that forskolin possesses multifaceted biological activities. This research paper have highlighted on phytochemical vis-a-vis histochemical localization of other terpenoids and forskolin in leaves of C. forskohlii. Qualitative analysis showed that terpenoids were present in leaves of the herb. Histochemical analysis of leaves of C. forskohlii shows that forskolin was found in the cells of palisade parenchyma, spongy parenchyma, and glandular trichomes of leaf in both the upper and lower epidermis. Thin layer chromatography of chloral hydrate washings showed presence of other terpenoids and forskolin in leaves of C. forskohlii. Rf value of forskolin was 0.6 and Rf values of other terpenoids were 0.62, 0.66, 0.86. This confirms that the yellowish-violet coloured vesicles seen in the sections of leaf contain the terpenoids. The leaves of the Indian drug plant are needed very badly to identify, isolate, design, develop, modify or to prepare new pharmacologically active compounds from the other terpenoids than forskolin. The mechanisms of action of various secondary metabolites isolated from this potential medicinal herb are yet to be elucidated. Leaves are significant (p<0.05) richer sources of antioxidant comparable with roots or tubers. The leaves can be used in place of tubers because they are significantly potent comparable with tubers and also we can ensure a continuous supply of the leaves without uprooting the plant for the tubers. This new finding of getting forskolin from leaves rather than sacrificing whole plant and tuber will help to conserve dwindling population of endangered important medicinal plant C. forskohlii vis-â-vis sustainable use of forskolin.
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Abstract (Original Language): 
Labdan yapılı diterpen olan forskolin, doğal olarak yetişen tıbbi bir bitki olan Coleus forskohlii Briq.'in etken maddesidir. Bilinen tek doğal diterpenoid forskolin kaynağıdır. Forskolin, bir biyolojik sistemdeki hemen hemen bütün hormona duyarlı adenilat siklaz enziminlerini aktive etme özelliğine sahiptir. Farmakolojik ve biyokimyasal çalışmalar forskolinin çok yönlü biyolojik aktivitelere sahip olduğunu göstermiştir. Bu çalışmada C. forskohlii yapraklarında diğer terpenoidlerin ve forskolinin histokimyasal ve fitokimyasal lokalizasyonu karşılıklı olarak açıklanmıştır. Yapılan nitel araştırma, bitkinin yapraklarında terpenoid bulunduğunu göstermiştir. C. forskohlii bitkisinin yaprakları üzerinde yapılan histokimyasal inceleme palizat parenkiması, sünger parenkiması hücrelerinde ve hem üst hem alt epidermisteki glandüler tüylerde forskolin bulunduğunu göstermiştir. Kloral hidratla yıkanan (materyallerin) ince tabaka kromatografisi C. forskohlii yapraklarında forskolin ve diğer terpenoidlerin bulunduğunu ortaya koymuştur. Forskolin için Rf değeri 0.6; diğer terpenoidler için Rf değerler ise 0.62, 0.66, 0.86 olarak bulunmuştur. Bu sonuç, yaprak seksiyonlarında görülen sarımsı-mor renkli keseciklerin terpenoid içerdiğini doğrulamaktadır. Forskolin dışında diğer terpenoidlerden farmakolojik olarak aktif bileşiklerin teşhis edilmesi, izole edilmesi, tasarlanması, geliştirilmesi, değiştirilmesi veya hazırlanması için Hindistan da tıbbi bitki yapraklarına büyük ölçüde ihtiyaç duyulmaktadır. Bu potansiyel tıbbi bitkiden izole edilen çeşitli sekonder metabolitlerin etki mekanizmaları henüz açıklığa kavuşmamıştır. Kök ve yumrulara kıyasla yapraklar önemli ölçüde daha zengin antioksidan kaynağıdır (p<0.05) Yapraklar yumrulara göre önemli ölçüde etkili oldukları için yumru yerine kullanılabilir. Ayrıca, yumru elde etme amacıyla bitkiyi kökünden sökmeden sürekli olarak yaprak elde edilebilmektedir. Bitkinin tamamının veya yumrularının feda edilmeden yapraklardan forskolin elde edilmesi ile ilgi bu yeni bulgu, sayıları giderek azalan soyu tükenmekte olan önemli bir tıbbi bitki olan C. forskohlii'nin sürdürülebilir şekilde forskolin kullanımına karşılık korunmasına yardımcı olacaktır.
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  • Turkish

REFERENCES

References: 

Abraham, Z., Srivastava, A.K., Bagchi, G.D. 1988. Cytoplasmic vesicles containing secondary metabolites in the root
of Coleus forskohlii. Current Science. 57: 1337-1339. Agarwal, K.C., Parks, R.E.Jr. 1983. Forskolin: A potential antimetastatic agent. International Journal of Cancer. 32:
801-804.
Akan, H., Korkut, M.M., Balos, M.M. 2008. An ethnobotanical study around Arat Mountain and its surroundings (Birecik, Şanlıurfa). Fırat University, Journal of Science and Engineering. 20: 67-81.
Allen, D.O., Ahmed, B., Naseer, K. 1986. Relationships between cyclic AMP levels and lipolysis in fat cells after isoproterenol and forskolin stimulation. The Journal of Pharmacology and Experimental Therapeutics. 238: 659¬664.
Ammon, H.P., Muller, A.B. 1984. Effect of forskolin on islet cyclic AMP, insulin secretion, blood glucose and intravenous glucose tolerance in rats. Naunyn Schmiedebergs Archives Pharmacology. 326: 364-367.
Bani,
B.
, Mavi, O., Adiguzel, N. 2011. Morphological and Anatomical Notes on a Local Endemic Species: Grammosciadium confertum Hub.-Mor. & Lamond (Umbelliferae). Biological Diversity and Conservation
(BioDiCon). 4/1: 1-6.
Bhat, S.V., Minale, L., Ricco, R., Zollo, F. 1993. Forskolin and congeners. In: Progress in the chemistry of organic natural products. (Eds. Herz, W., Kirby, G.W., Moore, R.E., Steglich, W., Tamm, C.H.), Springer-Verlag, New
York, pp. 1-60.
Cabi, E., Dogan, M., Mavi, O. 2010. Morphological and anatomical properties of the genus Crithopsis (Poaceae) in
Turkey. Biological Diversity and Conservation (BioDiCon). 3/2: 42-48. Cakilcioglu, U., Civelek, S. 2011. Flora of the region between copper mine and Tekevler village (Maden-Elazığ).
Biological Diversity and Conservation (BioDiCon). 4/1: 54-66. Caprioli, J., Sears, M., Bausher, L., Gregory, D., Mead, A. 1984. Forskolin lowers intraocular pressure by reducing
aqueous inflow. Investigative Ophthalmology and Visual Science. 25: 268-277. Combrinck, G.W., Plooy, DU., McCrindle, R.I., Botha, B.M. 2007. Morphology and Histochemistry of the glandular
trichomes of Lippia scaberrima (Verbenaceae). Annals in Botany. 99: 1111-1119. De Souza, N.J., Dohadwalla, A.N., Reden, U. 2006. Forskolin: A labdane diterpenoid with antihypertensive, positive
inotropic, platelet aggregation inhibitory, and adenylate cyclase activating properties. Medicinal Research Reviews.
3: 201-219.
De Souza, N.J., Shah, V. 1988. Forskolin - An adenylate cyclase activating drug from an Indian herb. Economic and
Medicinal Plant Researc. 2: 1-16. Dubey, M.P., Srimal, R.C., Nityanand, S., Dhawan, B.N. 1981. Pharmacological studies on coleonol, a hypotensive
diterpene from Coleus forskohlii. Journal of Ethnopharmacology. 3: 1-13. Duran, A., Martin, E., Ozturk, M., Cetin, O., Dinc, M., Ozdemir, A. 2010. Morphological, karyological and ecological
features of halophytic endemic Sphaerophysa kotschyana Boiss. (Fabaceae) in Turkey. Biological Diversity and
Conservation (BioDiCon). 3/2: 163-169.
Edeoga, H.O., Okwu, D.E., Mbaebie, B.O. 2005. Phytochemical constiuents of some Nigerian medicinal plants. African
Journal of Biotechnology. 4: 685-688. Engprasert, S., Taura, F., Kawamukai, M., Shoyama,Y. 2004. Molecular cloning and functional expression of
geranylgeranyl pyrophosphate synthatase from Coleus forskohlii. BMC Plant Biology. 4: 18-25. Gold, G., Qian, R.L., Grodsky, G.M. 1988. Insulin biosynthesis in HIT cells. Effects of glucose, forskolin, IBMX and
dexamethasone. Diabetes. 37: 160-165. Gupta, R. 1988. Genetic resources of medicinal plants. Indian Journal of Plant Genetic Resources. 1: 98-102. Hoshi, T., Garber, S.S., Aldrich, R.W. 1988. Effect of forskolin on voltage-gated K+ channels is independent of
adenylate cyclase activation. Science. 240: 1652-1655. Inamdar, P.K., Kanitkar, P.V., Reden, J., De Souza, N.J. 1984. Quantitative determination of forskolin by TLC and
HPLC. Planta Medica. 50: 30-34.
Jabeen, Q., Aslam, N. 2011. The pharmacological activities of prunes: The dried plums. Journal of Medicinal Plants
Research. 5/9: 1508-1511.
Kavitha, C., Rajamani, K., Vadivel, E. 2010. Coleus forskohlii: A comprehensive review on morphology, phytochemistry and pharmacological aspects. Journal of Medicinal Plants. 4: 278- 285.
Khatun, S., Cakilcioglu, U., Chatterjee, N.C. 2010. Phytochemical constituents vis-a-vis histochemical localization of forskolin in a medicinal plant Coleus forskohlii. Journal of Medicinal Plants Research. 5: 711-718.
Selima KHATUN et al., Pharmacognostic value of leaf anatomy and trichome morphology for identification of forskolin in a novel medicinal plant
Coleus forskohlii
Biological Diversity and Conservation - 4 / 2 (2011)
171
Khatun, S., Chatterjee, N.C., Cakilcioglu, U. 2011. Antioxidant activity of the medicinal plant Coleus forskohlii Briq.
African Journal of Biotechnology. 10: 2530-2535. Khatun, S., Chatterjee, N.C., Cakilcioglu, U. 2011a. The strategies for production of Forskolin vis-â-vis protection
against soil borne disease of the potential herb Coleus forskohlii Briq. European Journal of Medicinal Plants. 1: 1-9. Kilmer, S.L., Carlsen, R.C. 1984. Forskolin activation of adenylate cyclase in vivo stimulates nerve regeneration.
Nature. 307: 455-457.
Kivcak, B., Mert, T., Ertabaklar, H., Balcioglu, I.C., Toz, S.O. 2009. In vitro activity of Arbutus unedo against
Leishmania tropica promastigotes. Turkiye Parazitoloji Dergisi. 33/2: 114-115. Korkmaz, M., Ozcelik, H. 2011. Systematical and morphological characteristics of annual Gypsophila L.
(Caryophyllaceae) taxa of Turkey. Biological Diversity and Conservation (BioDiCon). 4/1: 79-98. Koyuncu, O., Yaylacı, O.K., Ozturk, D., Erkara, I.P., Savaroglu, F., Akcoskun, O., Ardic, M. 2010. Risk categories and
ethnobotanical features of the Lamiaceae taxa growing naturally in Osmaneli (Bilecik/Turkey) and environs.
Biological Diversity and Conservation (BioDiCon). 3/3: 31-45. Kramer, W., Thormann, J., Kindler, M., Schlepper, M. 1987. Effects of forskolin on left ventricular function in dilated
cardiomyopathy. Arzneimittel Forschung. 37: 364-367. Li, Z., Wang, J. 2006. A forskolin derivative, FSK88, induces apoptosis in human gastric cancer BGC823 cells through
caspase activation involving regulation of Bcl-2 family gene expression, dissipation of mitochondrial membrane
potential and cytochrome c release. Cell Biology International. 30: 940-946. Lichey, I., Friedrich, T., Priesnitz, M., Biamino, G., Usinger, P., Huckauf, H. 1984. Effect of forskolin on methacholine-
induced bronchoconstriction in extrinsic asthmatics. Lancet. 2: 167. Narayanan, P., Laddha, K.S., Akamanchi, K.G. 2002. Histochemical localization of forskolin and other terpenoids in
Coleus forskohlii. Current Science. 83: 945-946. Nourshargh, S., Hoult, J.R.S. 1986. Inhibition of human neurophil degranulation by forskolin in the presence of
phosphodiesterase inhibitors. European Journal of Pharmacology. 122: 205-212. Ozudogru, B., Akaydin, G., Erik, S.,Yesilada, E. 2011. Inferences from an ethnobotanical field expedition in the
selected locations of Sivas and Yozgat provinces (Turkey). Journal of Ethnopharmacology. Article in press. Rupp, R.H., De Souza, N.J., Dohadwalla, A.N. 1986. Proceedings of the International Symposium on Forskolin: Its
chemical, biological and medical potential. Hoechst India Limited, Bombay, pp. 19-30. Shah, V. 1996. Cultivation and utilization of medicinal plants (supplement), RRL and CSIR, Jammu - Tawai, pp. 385¬411.
Shan, Y., Xu, L., Lu, Y., Wang, X., Zheng, Q., Kong, L., Niwa, M. 2008. Diterpenes from Coleus forskohlii. Chemical
and Pharmaceutical Bulletin. 56: 52-56. Valdes, L.J., Mislankar, S.G., Paul, A.G. 1987. Coleus barbatus (C. forskohlii) (Lamiaceae) and the potential new drug
forskolin (Coleonol). Economic Botany. 41: 474-483. Yool, A.J., Stamer, W.D., Regan, J.W. 1996. Forskolin stimulation of water and cation permeability in aquaporin 1
water channels. Science. 273: 1216-1218.

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