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Ilaç Hammaddesi Olarak Sentezlenen Elektrofilik ve Nonelektrofilik Fenolik Schiff Bazlarının Umu Test Sistemi ile Genotoksik Aktivitesinin Araştırılması

Journal Name:

  • Çankaya University Journal of Science and Engineering

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
In this work, we investigated, through a short-term genotoxicity experiment, the umu-test, the mutagenic activities of a group of phenolic Schiff bases, namely Material A 2-(N-phenyliminomethyl)-phenol, Material B 2-(N-p-bromophenyliminomethyl)-phenol, Material C 2-(N-p-methylphenyliminomethyl)-phenol and Material D 2-(N-p-anilinephenyl iminomethyl)-phenol, the substances which we synthesized, in the chemistry laboratories of our university, to form the raw materials of medicines. The umu-test method is a colorimetric bacterial test system and has been developed on the basis of SOS response induced by DNA damage. Materials A, B, C and D and the experimental ma¬terials prepared in 5 different dose levels by dissolving these materials in DMSO have not increased the galactosidase enzyme activity. When calculated with respect to the enzyme unit calculation formula, results seemed to be not larger than the unit value of the positive mutagenic material 4NQO. Results have also been evaluated statistically.
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Abstract (Original Language): 
Bu çalışmamızda Üniversitemiz Kimya Bölümü laboratuarlarında sentez ettiğimiz ve ilac hammaddesi olarak kullanılmasını amaçladığımız fenolik Schiff bazlarının bir grubunu olusturan A maddesi 2-(N-feniliminometil)-fenol, B maddesi 2-(N-p-bromofeniliminometil)-fenol, C maddesi 2-(N-p-metilfeniliminometil)-fenol, D maddesi 2-(N-p-anilinfeniliminometil)-fenol'ü mutajenik aktiviteleri acısından kısa zamanlı geno-toksisite testlerinden olan umu-test sistemi ile arastırdık. Umu-test yontemi kalometrik bakteriyel bir test sistemidir ve DNA hasarı ile indüklenen SOS cevabı esas alınarak ge-listirilmistir. A, B, C, D maddeleri ve bunların DMSO'de cozülerek hazırlanan 5 farklı doz seviyelerindeki deney materyalleri galaktozidaz enzim aktivitesini artırmamıçstır. Sonuçclar enzim ünite hesaplama formülüne gore hesaplanınca pozitif mutajen olan 4NQO mad¬desinin uünite degğerinden fazla çcıkmamıçstır. Sonuçclar ayrıca istatistiki olarak da değgerlen-dirilmiçstir.
FULL TEXT (PDF): 
PDF icon arastirmax-ilac-hammaddesi-olarak-sentezlenen-elektrofilik-nonelektrofilik-fenolik-schiff-bazlarinin-umu-test-sistemi-ile-genotoksik-aktivitesinin-arastirilmasi.pdf
  • 2
75-86
  • Turkish

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