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AKUT AKCİĞER HASARINDA İNTRATRAKEAL DEKSAMETAZONUN LİPİD PEROKSİDASYONUNA ETKİLERİ

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Abstract (2. Language): 
The aim of our study was to show the effect of intratracheal (IT) dexamethasone on lipid peroxidation in hydrochloric acid (HCl) aspiration induced acute lung injury of rabbits. We measured the levels of malonyldialdehyde (MDA) which is end product of lipid peroxidation in plasma and bronchoalveolar lavage (BAL). Fourteen New Zeland rabbits were randomly assigned to 2 groups as the control group (CG, n=6) and dexamethasone group (DG, n=8). An endotracheal tube was placed trough a tracheostomy in all animals following ketamine hydrochloride (50 mg/kg, intramusculary) induced anesthesia. Maintenance of anesthesia was achived by the use of ketamine hydrochloride 10 mg/kg/h and atracurium besylate 1mg/kg/h. The animals were ventilated with pressure control mode for 5 hours and the parametres of ventilation were FiO2: 1.0, RR: 80/min, VT: 10 mL/kg, PEEP: 5 cmH2O. IT HCl (2mL/kg) was given following the initial sampling of MDA from plasma and BAL. Five minutes after the application of HCl the DG received IT dexamethasone (2.5mg/kg). Plasma and BAL resampling for the measurement of MDA was done at the second and fifth hour of HCl aspiration. Results.- The levels of MDA in the fifth hour samples of plasma and BAL were significantly different between two groups (p<0.01). (Mean plasma MDA levels of DG and CG were 1.14±0.6 nmol/mL and 2.5±1.36 nmol/mL, respectively; mean BAL MDA levels of DG and CG were 1.03±0.3 nmol/mL and 3.18±1.2 nmol/mL, respectively). Conclusion.- Intratracheal dexamethasone given after 5 minutes of HCl aspiration had a beneficial effect on the lipid peroxidation in acute lung injury of rabbits.
Abstract (Original Language): 
Hidroklorik asidin (HCl) intratrakeal (İT) verilmesiyle oluşturulan akut akciğer hasarında İT deksametazon uygulanması- nın lipid peroksidasyonuna etkisini araşt ırmayı amaçladık. Lipid peroksidasyonunu takip etmek içinde plazma ve bronkoalveolar lavaj sıvısında (BAL) son ürün olan malonidialdehid (MDA) düzeyinin öl- çümünden faydalandık. Ondört adet Yeni Zelanda tipi tavşan kontrol grubu (KG, n=6) ve deksametazon grubu (DG, n=8) olmak üzere rastgele iki gruba ayrıldı. Tüm hayvanlara ketamin hidroklorür (50 mg/kg, intramüsküler) ile anestezi uygulandıktan sonra trakeostomi açılarak endotrakeal tüp yerleştirildi. Anestezi idamesi 10 mg/kg/saat ketamin hidroklorid ve 1mg/kg/saat atrakuryum besilat infüzyonu ile sağlandı.Tüm tavşanlar, basınç kontrole modda, FiO2: 1.0, f: 80/dakika, PEEP: +5 cmH2O parametreleri ile 5 saat ventile edildiler. Tüm tavşanlardan başlangıç MDA ölçümleri için plazma ve BAL örneği alındıktan sonra 2 mL/kg’dan HCl İT verildi. DG’ye HCl uygulamas ından 5 dakika sonra 2.5 mg/kg İT deksametazon verildi. Asid aspirasyonunda 2 ve 5 saat sonra MDA tayini için tekrar plazma ve BAL örnekleri alındı. Gruplar arası, plazma ve BAL MDA düzeylerinde 5.saatte istatistiki olarak ileri derecede anlamlı fark bulundu (p<0.01), (KG’de plazma MDA düzeyi ortalaması 2.5±1.36 nmol/mL, DG’de 1.14±0.6 nmol/ mL olarak bulundu. KG’de BAL MDA dü- zeyi ortalaması 3.18±1.2 nmol/mL iken DG’de 1.03±0.3 nmol/mL olarak bulundu). Sonuç olarak; intratrakeal HCl uygulamas ından 5 dakika sonra intratrakeal deksametazon uygulanmasının lipid peroksidasyonunu azalttığı kanısına vard ık.

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